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Rpl12 (NM_009076) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201323
Rpl12 (Myc-DDK-tagged) - Mouse ribosomal protein L12 (Rpl12)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpl12
Synonyms E430018F03
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201323 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCCAAGTTCGACCCCAACGAGGTCAAAGTCGTGTACCTGAGGTGCACCGGAGGCGAGGTCGGCG
CCACATCCGCCTTGGCCCCGAAGATCGGTCCTCTGGGTCTGTCTCCGAAGAAAGTTGGCGATGACATTGC
CAAGGCTACCGGTGACTGGAAGGGTCTCAGAATTACAGTGAAACTGACCATCCAGAACAGACAGGCCCAG
ATCGAGGTGGTGCCTTCTGCCTCAGCCCTGATCATCAAAGCCCTCAAGGAGCCACCGAGAGACAGGAAGA
AGCAGAAGAACATTAAACACAGTGGGAACATTACTTTTGATGAGATTGTCAACATTGCCCGGCAGATGAG
ACACCGGTCTTTGGCTAGAGAACTTTCTGGAACTATCAAGGAGATCCTGGGTACTGCACAGTCTGTGGGC
TGCAATGTGGATGGCCGCCACCCTCATGACATCATAGATGACATCAACAGTGGTGCAGTGGAGTGCCCAG
CTAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201323 protein sequence
Red=Cloning site Green=Tags(s)

MPPKFDPNEVKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQ
IEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVG
CNVDGRHPHDIIDDINSGAVECPAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009076
ORF Size 495 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009076.3, NP_033102.2
RefSeq Size 643 bp
RefSeq ORF 498 bp
Locus ID 269261
UniProt ID P35979
Cytogenetics 2 B
MW 17.8 kDa
Summary Binds directly to 26S ribosomal RNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009076" in other vectors (3)
SKU Description Size Price
MC207941 Rpl12 (untagged) - Mouse ribosomal protein L12 (Rpl12), (10ug) 10 ug
$165.00
MR201323L3 Lenti ORF clone of Rpl12 (Myc-DDK-tagged) - Mouse ribosomal protein L12 (Rpl12) 10 ug
$450.00
MR201323L4 Lenti ORF clone of Rpl12 (mGFP-tagged) - Mouse ribosomal protein L12 (Rpl12) 10 ug
$450.00

Plasmid Map for MR201323

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201323
Circular map for MR201323

file-alt Citations

The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress
Kobiita, Godbersen, Araldi et al
Cell Rep (2020) 32 (1), 107846 DOI: 10.1016/j.celrep.2020.107846
Cited by 59
AI Summary: In this study, OriGene’s Myc-DDK-tagged expression plasmids for Nop56, Tsr3, Rps2, Rps11, Rps14, Rps15, Rps20, Rpl10a, Rpl12, Rpl13, and Rpl13a were used to investigate the role of specific ribosomal proteins and factors in elucidating how JAZF1 regulates ribosome biogenesis and function in pancreatic β-cells under metabolic stress.
Snippet: Jazf1 adenovirus: Ad: Jazf1-HA-tagged This paper N/A Gfp adenovirus: Ad:GFP This paper N/A Nop56 (Myc-DDK-tagged) Origene MR209091 Tsr3 (Myc-DDK-tagged) Origene MR204683 Rps2 (Myc-DDK-tagged) Origene MR204027 Rps11 (Myc-DDK-tagged) Origene MR216516 Rps14 (Myc-DDK-tagged) Origene RC203889 Rps15 (Myc-DDK-tagged) Origene MR200959 Rps20 (Myc-DDK-tagged) Origene MR200561 Rpl10a (Myc-DDK-tagged) Origene MR200782 Rpl12 (Myc-DDK-tagged) Origene MR201323 Rpl13 (Myc-DDK-tagged) Origene MR202263 Rpl13a (Myc-DDK-tagged) Origene MR202103
Showing 1-1 of 1 papers.
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