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Ubd (NM_023137) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201267
Ubd (Myc-DDK-tagged) - Mouse ubiquitin D (Ubd)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ubd
Synonyms FAT10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201267 representing NM_023137
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCTGTCCGCACCTGTGTTGTCCGTTCAGACCAATGGCGGTTAATGACCTTTGAGACCACTGAGA
ATGACAAAGTGAAGAAGATAAATGAACATATTAGGTCCCAAACCAAGGTCTCTGTACAGGACCAGATCCT
TCTGCTAGACTCCAAAATCCTCAAGCCCCATCGAAAATTGTCATCCTATGGGATTGACAAGGAAACCACT
ATCCACCTTACCCTGAAGGTGGTGAAGCCCAGTGATGAAGAGCTGCCCTTGTTTCTGGTGGAGTCCAAAA
ACGAGGGGCAAAGGCACCTCCTCCGAGTTCGAAGATCCAGCTCAGTGGCCCAGGTGAAAGAGATGATCGA
GAGTGTGACCTCTGTGATCCCTAAGAAGCAGGTTGTGAATTGCAACGGAAAGAAGCTGGAAGATGGAAAG
ATCATGGCTGACTACAACATCAAGAGTGGCAGTTTGCTCTTTCTGACAACACACTGCACTGGGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201267 representing NM_023137
Red=Cloning site Green=Tags(s)

MASVRTCVVRSDQWRLMTFETTENDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRKLSSYGIDKETT
IHLTLKVVKPSDEELPLFLVESKNEGQRHLLRVRRSSSVAQVKEMIESVTSVIPKKQVVNCNGKKLEDGK
IMADYNIKSGSLLFLTTHCTGG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023137
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023137.3, NP_075626.1
RefSeq Size 1006 bp
RefSeq ORF 489 bp
Locus ID 24108
UniProt ID P63072
Cytogenetics 17 B1
MW 18.8 kDa
Summary Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Probably functions as a survival factor. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases.UniProtKB/Swiss-Prot Function
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"NM_023137" in other vectors (4)
SKU Description Size Price
MC207459 Ubd (untagged) - Mouse ubiquitin D (Ubd), (10ug) 10 ug
$165.00
MG201267 Ubd (tGFP-tagged) - Mouse ubiquitin D (Ubd) 10 ug
$350.00
MR201267L3 Lenti ORF clone of Ubd (Myc-DDK-tagged) - Mouse ubiquitin D (Ubd) 10 ug
$450.00
MR201267L4 Lenti ORF clone of Ubd (mGFP-tagged) - Mouse ubiquitin D (Ubd) 10 ug
$450.00

Plasmid Map for MR201267

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201267
Circular map for MR201267

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