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Mrpl30 (NM_027098) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201246
Mrpl30 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L30 (Mrpl30), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl30
Synonyms 2310001L22Rik; MRP-L28; Rpml28
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201246 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGGGTTCTGCGCTCCGCGTTCCAGAGGCCTCCATGCAGACTGCAGACTGTGAAGAAAGGTGCAG
AATCTCTCATTGGTACAGAATGGATTCGTCACAAATTTACCAAATCAAGAATTCCAGATAAAGTGTTTCA
GCCTAAACCTGAAGATCATGAAAAGTATGGTGGGGACCCCCAGAACCCTCACAAACTGCACATTGTCACA
AGAATAAGAAGTACAAAGAGGCGTCCATACTGGGAAAAAGATACCATCAAGATGCTTGGACTACAGAAGG
CACACAGCCCTCAAATTCACAAGAACATCCCATCGGTGAATGCAAAGCTGAAAGTGGTTAAACACTTGAT
ACGCATCCAGCCTCTGAAGCTGCCACAGGGACTTCCCACAGAAGAGACCATGTCCAGCACATGCCTCAAG
AGCACTGGGGAGTTGGTCGTGCAGTGGCATCTGAAGCCCGTGGAGCAGGAAGCGAAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201246 protein sequence
Red=Cloning site Green=Tags(s)

MAGVLRSAFQRPPCRLQTVKKGAESLIGTEWIRHKFTKSRIPDKVFQPKPEDHEKYGGDPQNPHKLHIVT
RIRSTKRRPYWEKDTIKMLGLQKAHSPQIHKNIPSVNAKLKVVKHLIRIQPLKLPQGLPTEETMSSTCLK
STGELVVQWHLKPVEQEAKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027098
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027098.1, NM_027098.2, NP_081374.1
RefSeq Size 691 bp
RefSeq ORF 483 bp
Locus ID 107734
UniProt ID Q9D7N6
Cytogenetics 1 B
MW 18.4 kDa
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"NM_027098" in other vectors (4)
SKU Description Size Price
MC207825 Mrpl30 (untagged) - Mouse mitochondrial ribosomal protein L30 (Mrpl30), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$165.00
MG201246 Mrpl30 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L30 (Mrpl30) 10 ug
$350.00
MR201246L3 Lenti ORF clone of Mrpl30 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L30 (Mrpl30), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR201246L4 Lenti ORF clone of Mrpl30 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L30 (Mrpl30), nuclear gene encoding mitochondrial protein 10 ug
$450.00

Plasmid Map for MR201246

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201246
Circular map for MR201246

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