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Cplx3 (NM_146223) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201191
Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cplx3 or NM_146223"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cplx3
Synonyms BC016632; CpxIII; ERGIC-53L; ERGL; Lamn1l; Lman1l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201191 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTCATGGTGAAGTCCATGGTGGGTGGCCAACTGAAGAACCTCACAGGGAGCCTGGGGGGCGGCG
AGGACAAGGGGGACGGAGACAAGTCTGCAGCCGAAGCACAGGGCATGAGCCGAGAAGAGTACGAGGAGTA
TCAGAAGCAACTGGTGGAGGAGAAGATGGAGCGAGATGCACAGTTCACACAGAGGAAGGCAGAGCGGGCC
ACACTGCGAAGTCACTTCAGAGACAAATACCGTCTGCCCAAGAACGAAACAGATGAAAGCCAGATCCAGC
TGGCAGGCGGAGATGTGGAGCTGCCTCGGGAGCTAGCCAAGATGATCGAGGAGGATACGGAGGAAGAGGA
GGATAAGGCCTCTGTGCTCGGGCAGCTGGCCAGCCTCCCTGGCCTAGACCTCAGCTCACTCAAGGACAAA
GCCCAGACTACACTAGGGGACCTCAAGCAATCAGCTGAGAAGTGCCATATCATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201191 protein sequence
Red=Cloning site Green=Tags(s)

MAFMVKSMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERA
TLRSHFRDKYRLPKNETDESQIQLAGGDVELPRELAKMIEEDTEEEEDKASVLGQLASLPGLDLSSLKDK
AQTTLGDLKQSAEKCHIM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146223
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146223.3
RefSeq Size 2859 bp
RefSeq ORF 477 bp
Locus ID 235415
UniProt ID Q8R1B5
Cytogenetics 9 B
MW 17.6 kDa
Summary Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (PubMed:19386896). Required for the maintenance of synaptic ultrastructure in the adult retina (PubMed:19386896). Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (PubMed:15911881, PubMed:19386896, PubMed:27335398). Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+) vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+) vesicle release (PubMed:22694764, PubMed:27335398).UniProtKB/Swiss-Prot Function
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"NM_146223" in other vectors (5)
SKU Description Size Price
MC201944 Cplx3 (untagged) - Mouse complexin 3 (Cplx3), (10ug) 10 ug
$150.00
MG201191 Cplx3 (tGFP-tagged) - Mouse complexin 3 (Cplx3) 10 ug
$350.00
MR201191L3 Lenti ORF clone of Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3) 10 ug
$450.00
MR201191L4 Lenti ORF clone of Cplx3 (mGFP-tagged) - Mouse complexin 3 (Cplx3) 10 ug
$450.00
MR201193 Cplx3 (Myc-DDK-tagged) - Mouse complexin 3 (Cplx3) 10 ug
$289.00

Plasmid Map for MR201191

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201191
Circular map for MR201191

file-alt Citations

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