Lmo1 (NM_057173) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201171
Lmo1 (Myc-DDK-tagged) - Mouse LIM domain only 1 (Lmo1)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lmo1
Synonyms Rbtn-1; Rbtn1; Ttg1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201171 representing NM_057173
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGTGCTGGACAAGGAGGACGGTGTGCCGATGCTCTCCGTCCAACCTAAGGGGAAACAGAAGGGCT
GCGCAGGCTGCAACCGCAAGATCAAGGACCGGTACCTGCTGAAGGCACTGGACAAGTACTGGCATGAGGA
CTGCCTCAAGTGTGCCTGCTGTGACTGTCGCCTGGGCGAGGTGGGCTCCACTCTCTACACCAAGGCCAAC
CTCATCCTGTGCCGGCGTGACTACCTGAGGCTTTTTGGCACCACAGGAAACTGTGCTGCCTGCAGCAAGC
TGATCCCAGCTTTTGAGATGGTGATGCGGGCCCGAGACAATGTGTATCACCTTGACTGCTTCGCCTGCCA
GCTCTGCAATCAGAGATTTTGCGTGGGAGACAAATTCTTCCTGAAGAACAACATGATCTTGTGCCAGGTG
GACTATGAGGAGGGGCATCTCAATGGCACCTTTGAATCCCAGGTTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201171 representing NM_057173
Red=Cloning site Green=Tags(s)

MMVLDKEDGVPMLSVQPKGKQKGCAGCNRKIKDRYLLKALDKYWHEDCLKCACCDCRLGEVGSTLYTKAN
LILCRRDYLRLFGTTGNCAACSKLIPAFEMVMRARDNVYHLDCFACQLCNQRFCVGDKFFLKNNMILCQV
DYEEGHLNGTFESQVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_057173
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_057173.3, NP_476514.1
RefSeq Size 1289 bp
RefSeq ORF 471 bp
Locus ID 109594
UniProt ID Q924W9
Cytogenetics 7 57.21 cM
MW 17.8 kDa
Summary May be involved in gene regulation within neural lineage cells potentially by direct DNA binding or by binding to other transcription factors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_057173" in other vectors (4)
SKU Description Size Price
MC203585 Lmo1 (untagged) - Mouse LIM domain only 1 (Lmo1), (10ug) 10 ug
$150.00
MG201171 Lmo1 (tGFP-tagged) - Mouse LIM domain only 1 (Lmo1) 10 ug
$350.00
MR201171L3 Lenti ORF clone of Lmo1 (Myc-DDK-tagged) - Mouse LIM domain only 1 (Lmo1) 10 ug
$450.00
MR201171L4 Lenti ORF clone of Lmo1 (mGFP-tagged) - Mouse LIM domain only 1 (Lmo1) 10 ug
$450.00

Plasmid Map for MR201171

Download sequence for

Cloning scheme for MR201171

Circular map for MR201171

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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