OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Npc2 (NM_023409) Mouse Tagged Lenti ORF Clone

  • LentiORF®
Be the first to review this product
SKU
MR201050L3
Lenti ORF clone of Npc2 (Myc-DDK-tagged) - Mouse Niemann Pick type C2 (Npc2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $450.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA311515 Goat Anti-Npc2 (mouse) (aa21-33) Antibody
100 ug
$565.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00
GE100111 Forward primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
GE100128 Reverse primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
TR30037 Lenti-vpak packaging kit - packaging plasmids and transfection reagent
10 reactions
$720.00

Other products for "Npc2 or NM_023409"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npc2
Synonyms 2700012J19Rik; AA408070; AU045843; HE1
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR201050).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023409
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023409.4, NP_075898.1
RefSeq Size 3278 bp
RefSeq ORF 450 bp
Locus ID 67963
UniProt ID Q9Z0J0
Cytogenetics 12 D1
Summary Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:12591949, PubMed:17018531, PubMed:21315718, PubMed:26296895). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (By similarity). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (PubMed:21315718).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_023409" in other vectors (4)
SKU Description Size Price
MC200435 Npc2 (untagged) - Mouse Niemann Pick type C2 (Npc2), (10ug) 10 ug
$150.00
MG201050 Npc2 (tGFP-tagged) - Mouse Niemann Pick type C2 (Npc2) 10 ug
$489.00
MR201050 Npc2 (Myc-DDK-tagged) - Mouse Niemann Pick type C2 (Npc2) 10 ug
$289.00
MR201050L4 Lenti ORF clone of Npc2 (mGFP-tagged) - Mouse Niemann Pick type C2 (Npc2) 10 ug
$450.00

Plasmid Map for MR201050L3

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201050L3
Circular map for MR201050L3

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products