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Gapdh (BC096042) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200822
Gapdh (Myc-DDK-tagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
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Other products for "Gapdh or BC096042"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gapdh
Synonyms Gapd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC096042
ORF Size 408 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC096042.1
RefSeq Size 635 bp
RefSeq ORF 410 bp
Locus ID 14433
Cytogenetics 6 59.32 cM
MW 23.2 kDa
Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. provided by RefSeq, Jan 2014
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"BC096042" in other vectors (4)
SKU Description Size Price
MC207054 Gapdh (untagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416), (10ug) 10 ug
$150.00
MG200822 Gapdh (tGFP-tagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) 10 ug
$489.00
MR200822L3 Lenti ORF clone of Gapdh (Myc-DDK-tagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) 10 ug
$450.00
MR200822L4 Lenti ORF clone of Gapdh (mGFP-tagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase (cDNA clone MGC:107018 IMAGE:1067416) 10 ug
$450.00

Plasmid Map for MR200822

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Cloning scheme for MR200822
Circular map for MR200822

file-alt Citations

Energy determinants GAPDH and NDPK act as genetic modifiers for hepatocyte inclusion formation
Snider, Weerasinghe, Singla et al
J. Cell Biol. (2011) 195 (2), 217-29 DOI: 10.1083/jcb.201102142
Cited by 36
AI Summary: OriGene products, specifically the GAPDH shRNA (TG502386) and NME1/NDPK shRNA (TG514655), were used in this study to knock down GAPDH and NDPK expression in hepatocytes, enabling the authors to investigate the roles of these proteins as genetic modifiers in DDC-induced liver injury.
Snippet: and isolated hepatocytes. C57BL livers and hepatocytes also manifested significant down-regulation, aggregation, and nuclear translocation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH knockdown depleted
FigureFigureFigure
Showing 1-1 of 1 papers.
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