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Cyb5a (NM_025797) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200799
Cyb5 (Myc-DDK-tagged) - Mouse cytochrome b-5 (Cyb5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cyb5a or NM_025797"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cyb5a
Synonyms 0610009N12Rik; Cyb5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200799 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGCAGTCAGACAAGGATGTGAAGTACTACACCCTGGAAGAGATTCAGAAGCACAAAGACAGCA
AGAGCACCTGGGTGATCCTGCATCATAAGGTGTACGATCTGACCAAGTTTCTCGAAGAGCATCCTGGTGG
AGAAGAAGTCCTAAGAGAGCAAGCTGGGGGTGATGCTACCGAGAATTTTGAGGACGTCGGGCACTCTACG
GATGCACGAGAACTGTCCAAAACATACATCATCGGGGAACTCCATCCAGATGACAGATCAAAGATAGCCA
AGCCTTCGGATACTCTTATCACTACTGTGGAGTCTAATTCCAGTTGGTGGACCAACTGGGTGATCCCAGC
CATCTCAGCCCTGGCTGTAGCTCTGATGTATCGCCTCTACATGGCAGAAGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200799 protein sequence
Red=Cloning site Green=Tags(s)

MAGQSDKDVKYYTLEEIQKHKDSKSTWVILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHST
DARELSKTYIIGELHPDDRSKIAKPSDTLITTVESNSSWWTNWVIPAISALAVALMYRLYMAED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025797
ORF Size 402 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025797.4
RefSeq Size 749 bp
RefSeq ORF 405 bp
Locus ID 109672
UniProt ID P56395
Cytogenetics 18 57.53 cM
MW 15.2 kDa
Summary Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.UniProtKB/Swiss-Prot Function
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"NM_025797" in other vectors (4)
SKU Description Size Price
MC202110 Cyb5 (untagged) - Mouse cytochrome b-5 (Cyb5), (10ug) 10 ug
$150.00
MG200799 Cyb5 (tGFP-tagged) - Mouse cytochrome b-5 (Cyb5) 10 ug
$350.00
MR200799L3 Lenti ORF clone of Cyb5 (Myc-DDK-tagged) - Mouse cytochrome b-5 (Cyb5) 10 ug
$450.00
MR200799L4 Lenti ORF clone of Cyb5 (mGFP-tagged) - Mouse cytochrome b-5 (Cyb5) 10 ug
$450.00

Plasmid Map for MR200799

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200799
Circular map for MR200799

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