Fabp3 (NM_010174) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR200776
Fabp3 (Myc-DDK-tagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fabp3
Synonyms Fabph-1; Fabph-4; Fabph1; Fabph4; H-FABP; Mdgi
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200776 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGCCTTTGTCGGTACCTGGAAGCTAGTGGACAGCAAGAATTTTGATGACTACATGAAGTCAC
TCGGTGTGGGCTTTGCCACCAGGCAGGTGGCTAGCATGACCAAGCCTACTACCATCATCGAGAAGAACGG
GGATACTATCACCATAAAGACACAAAGTACCTTCAAGAACACAGAGATCAACTTTCAGCTGGGAATAGAG
TTCGACGAGGTGACAGCAGATGACCGGAAGGTCAAGTCACTGGTGACGCTGGACGGAGGCAAACTCATCC
ATGTGCAGAAGTGGAACGGGCAGGAGACAACACTAACTAGGGAGCTAGTTGACGGGAAACTCATCCTGAC
TCTCACTCATGGCAGTGTGGTGAGCACTCGGACTTATGAGAAGGAGGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR200776 protein sequence
Red=Cloning site Green=Tags(s)

MADAFVGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDTITIKTQSTFKNTEINFQLGIE
FDEVTADDRKVKSLVTLDGGKLIHVQKWNGQETTLTRELVDGKLILTLTHGSVVSTRTYEKEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010174
ORF Size 399 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010174.2
RefSeq Size 669 bp
RefSeq ORF 402 bp
Locus ID 14077
UniProt ID P11404
Cytogenetics 4 63.43 cM
MW 14.8 kDa
Summary FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.UniProtKB/Swiss-Prot Function
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Documents
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"NM_010174" in other vectors (4)
SKU Description Size Price
MC200344 Fabp3 (untagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3), (10ug) 10 ug
$150.00
MG200776 Fabp3 (tGFP-tagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3) 10 ug
$489.00
MR200776L3 Lenti ORF clone of Fabp3 (Myc-DDK-tagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3) 10 ug
$450.00
MR200776L4 Lenti ORF clone of Fabp3 (mGFP-tagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3) 10 ug
$450.00

Plasmid Map for MR200776

Download sequence for

Cloning scheme for MR200776

Circular map for MR200776

file-alt Citations

Heart-Type Fatty Acid Binding Protein Binds Long-Chain Acylcarnitines and Protects against Lipotoxicity
Zelencova-Gopejenko, Videja, Grandane et al
Int J Mol Sci (2023) 24 (6) DOI: 10.3390/ijms24065528
Cited by 13
AI Summary: OriGene’s mouse FABP3 (NM_010174) tagged ORF clone plasmid was used to transfect cells, enabling the study of FABP3’s role in binding long-chain acylcarnitines and protecting against lipotoxicity.
Snippet: viability of the PANC-1 cells. The commercially available FuGENE HD transfection reagent (Promega Corporation, Madison, WI, USA) was mixed with the FABP3 (NM_010174) mouse tagged ORF clone plasmid (OriGene Technologies, Inc., Rockville, MD, USA) in a 4:1 ratio for transfection according to the manufacturer’s protocol. Transfection was performed for 24 h in 6-well plates. To ensure transfection
FigureFigureFigure
Showing 1-1 of 1 papers.
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