Saa4 (NM_011316) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR200725
Saa4 (Myc-DDK-tagged) - Mouse serum amyloid A 4 (Saa4)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Saa4
Synonyms Saa-4; Saa-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200725 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCTTGCCACCGTCATTGTCCTCTGTTCTTTGTTCCTGGGAGTCAGTGGTGATGGCTGGTATTCAT
TCTTCAGGGAAGCCGTACAAGGGACTTGGGACTTGTGGAGAGCCTATCGGGACAACCTAGAAGCTAATTA
CCAAAATGCAGACCAATACTTCTATGCTCGTGGGAACTACGAGGCCCAACAGAGGGGCTCTGGGGGCATC
TGGGCTGCTAAAATAATCAGCACCAGCAGGAAATACTTCCAGGGCCTCCTAAACCGATATTATTTTGGAA
TCAGGAACCATGGATTGGAAACCCTGCAGGCTACCCAGAAAGCCGAGGAATGGGGCCGCAGTGGCAAAAA
CCCCAATCACTTCCGACCCGAGGGCCTTCCTGAGAAGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR200725 protein sequence
Red=Cloning site Green=Tags(s)

MRLATVIVLCSLFLGVSGDGWYSFFREAVQGTWDLWRAYRDNLEANYQNADQYFYARGNYEAQQRGSGGI
WAAKIISTSRKYFQGLLNRYYFGIRNHGLETLQATQKAEEWGRSGKNPNHFRPEGLPEKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011316
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011316.4
RefSeq Size 2067 bp
RefSeq ORF 393 bp
Locus ID 20211
UniProt ID P31532
Cytogenetics 7 30.53 cM
MW 15.1 kDa
Summary Major acute phase reactant. Apolipoprotein of the HDL complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011316" in other vectors (4)
SKU Description Size Price
MC202982 Saa4 (untagged) - Mouse serum amyloid A 4 (Saa4), (10ug) 10 ug
$150.00
MG200725 Saa4 (tGFP-tagged) - Mouse serum amyloid A 4 (Saa4) 10 ug
$489.00
MR200725L3 Lenti ORF clone of Saa4 (Myc-DDK-tagged) - Mouse serum amyloid A 4 (Saa4) 10 ug
$450.00
MR200725L4 Lenti ORF clone of Saa4 (mGFP-tagged) - Mouse serum amyloid A 4 (Saa4) 10 ug
$450.00

Plasmid Map for MR200725

Download sequence for

Cloning scheme for MR200725

Circular map for MR200725

file-alt Citations

Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases
Mohanty, Miličević, Göthert et al
Front Immunol (2025) 16, 1544085 DOI: 10.3389/fimmu.2025.1544085
Cited by 22
AI Summary: OriGene products—specifically the Myc-DDK tagged mouse ORF expression plasmids for SAA1 (MR200611), SAA2 (MR219150), SAA3 (MR200605), and SAA4 (MR200725)—were used to overexpress SAA proteins in macrophages, enabling the authors to study their roles in regulating inflammation and bacterial clearance.
Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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