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Ccdc134 (NM_172428) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200630
Ccdc134 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 134 (Ccdc134)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc134
Synonyms 2310042L06Rik; AW208859
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200630 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTTCTTCAGTTCCTGGCAGCCTTCTCTGTCCTCCTGTGGCCTGGCACGGAAGTCACAGGCGCCC
TGAAGAGCACTCTGGACCCGAGCCTCAAGATTTACAAGAAGATGTTTGAGGTGAAGCGGCGAGAGCAGCT
GTTGGCACTGAAGAACCTGGCGCAGCTGAATGACATCCACCAGCAGTACAAGATCCTGGATGTCATGCTT
AAGGGACTCTTCAAGGTGCTGGAGGACTCCCGCACAGTGCTCATTGCTGCCGATGTGCTTCCAGACGGGC
CGGTCCCCCAGGATGAGAAGCTGAAGGATGCTTTCTCGCACGTGGTGGAGAACACAGCCTTTTTTGGTGA
CGTCGTGCTGCGCTTCCCAAAGATTGTCCACCACTACTTTGACCATAATTCCAACTGGAACCTCCTGATC
CGCTGGGGCATCAGCTTCTGCAACCAGACAGGCGTCTTTGACCAGGGGCCCCACTCACCCATCCTCAGCC
TGATGGCCCAGGAGCTGGGGATCACTGAGAAAGATTCTGACTTCCGGAACCCGTTTAAAACAGACCAGAC
AGAGTTTATTCCTAGCACCGACCCTTTCCAGAAGGCTTTGAGGGAAGAAGAAAAACGCCGCAAGAAGGAG
GAGAGGCGGAAGGAGATCCGGAAAGGCCCGAGGATTTCAAGATCCCAGTCTGAGTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200630 protein sequence
Red=Cloning site Green=Tags(s)

MDLLQFLAAFSVLLWPGTEVTGALKSTLDPSLKIYKKMFEVKRREQLLALKNLAQLNDIHQQYKILDVML
KGLFKVLEDSRTVLIAADVLPDGPVPQDEKLKDAFSHVVENTAFFGDVVLRFPKIVHHYFDHNSNWNLLI
RWGISFCNQTGVFDQGPHSPILSLMAQELGITEKDSDFRNPFKTDQTEFIPSTDPFQKALREEEKRRKKE
ERRKEIRKGPRISRSQSEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172428
ORF Size 687 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172428.2, NP_766016.2
RefSeq Size 2073 bp
RefSeq ORF 690 bp
Locus ID 76457
UniProt ID Q8C7V8
Cytogenetics 15 E1
MW 26.5 kDa
Summary In extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function. Enhances cytotoxic anti-tumor activity of CD8(+) T cells. May inhibit ERK and JNK signaling activity. May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling.UniProtKB/Swiss-Prot Function
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"NM_172428" in other vectors (3)
SKU Description Size Price
MG200630 Ccdc134 (tGFP-tagged) - Mouse coiled-coil domain containing 134 (Ccdc134) 10 ug
$500.00
MR200630L3 Lenti ORF clone of Ccdc134 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 134 (Ccdc134) 10 ug
$600.00
MR200630L4 Lenti ORF clone of Ccdc134 (mGFP-tagged) - Mouse coiled-coil domain containing 134 (Ccdc134) 10 ug
$600.00

Plasmid Map for MR200630

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Cloning scheme for MR200630
Circular map for MR200630

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