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Ndufb2 (NM_026612) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200361
Ndufb2 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (Ndufb2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufb2
Synonyms 8kDa; 1810011O01Rik; AGGG; AI325567; CI-AGGG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200361 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCGCTCACCCGTCTGGTACCTTTCGGCCGCGTCGGAGGCCGCCTGCTCCGGGGCTGCCGCGCGC
GGGCGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTACATATTCAGCCCCGGTACAGGGAGTT
TCCCCAGCTTACCCGGTCCCAGGTGATCCAGGGTGAGTTCTTAAGCTCACTCATGTGGTTCTGGATTCTT
TGGCGATTTTGGCATGACTCGGATGCTGTGCTGGGTCACTTTTCATATCCAGATCCTTCACAGTGGACAG
ATGAAGAACTGGGAATCCCTCCTGATGACGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200361 protein sequence
Red=Cloning site Green=Tags(s)

MSALTRLVPFGRVGGRLLRGCRARAAGDSGVRHAGGGVHIQPRYREFPQLTRSQVIQGEFLSSLMWFWIL
WRFWHDSDAVLGHFSYPDPSQWTDEELGIPPDDED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026612
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026612.4
RefSeq Size 455 bp
RefSeq ORF 318 bp
Locus ID 68198
UniProt ID Q9CPU2
Cytogenetics 6 B1
MW 12 kDa
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
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"NM_026612" in other vectors (4)
SKU Description Size Price
MC203820 Ndufb2 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (Ndufb2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MG200361 Ndufb2 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (Ndufb2) 10 ug
$350.00
MR200361L3 Lenti ORF clone of Ndufb2 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (Ndufb2), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200361L4 Lenti ORF clone of Ndufb2 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (Ndufb2), nuclear gene encoding mitochondrial protein 10 ug
$450.00

Plasmid Map for MR200361

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200361
Circular map for MR200361

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