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Vamp3 (NM_009498) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200332
Vamp3 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 3 (Vamp3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vamp3
Synonyms ceb; D130027G05Rik; VAMP-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200332 representing NM_009498
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACAGGTGTGCCTTCGGGGTCAAGTGCTGCCACTGGCAGTAATCGAAGACTCCAGCAGACACAAA
ATCAAGTAGATGAGGTGGTTGACATCATGAGAGTCAATGTGGATAAGGTGTTAGAAAGAGACCAGAAGCT
CTCGGAGCTAGATGACCGCGCAGATGCACTGCAGGCAGGTGCCTCGCAGTTTGAAACAAGTGCTGCCAAG
TTGAAGAGAAAGTATTGGTGGAAGAACTGCAAGATGTGGGCGATAGGGATCAGTGTCCTGGTGATCATTG
TCATCATCATCATCGTGTGGTGTGTCTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200332 representing NM_009498
Red=Cloning site Green=Tags(s)

MSTGVPSGSSAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAK
LKRKYWWKNCKMWAIGISVLVIIVIIIIVWCVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009498
ORF Size 309 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009498.4, NP_033524.1
RefSeq Size 2115 bp
RefSeq ORF 312 bp
Locus ID 22319
UniProt ID P63024
Cytogenetics 4 E2
MW 11.9 kDa
Summary SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.UniProtKB/Swiss-Prot Function
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Documents
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"NM_009498" in other vectors (4)
SKU Description Size Price
MC209629 Vamp3 (untagged) - Mouse vesicle-associated membrane protein 3 (Vamp3), (10ug) 10 ug
$165.00
MG200332 Vamp3 (tGFP-tagged) - Mouse vesicle-associated membrane protein 3 (Vamp3) 10 ug
$489.00
MR200332L3 Lenti ORF clone of Vamp3 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 3 (Vamp3) 10 ug
$450.00
MR200332L4 Lenti ORF clone of Vamp3 (mGFP-tagged) - Mouse vesicle-associated membrane protein 3 (Vamp3) 10 ug
$450.00

Plasmid Map for MR200332

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200332
Circular map for MR200332

file-alt Citations

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