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1810007E14Rik (BC031454) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200233
1810007E14Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810007E14 gene (cDNA clone MGC:25888 IMAGE:4216482)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1810007E14Rik
Synonyms 1810007E14Rik; AA238765
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200233 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCCAGGTGGCAAGGGGCATTGTGGGCCACCCCCTGGCCATGGTCCGGGACACTGTCATGAGGGAC
ATCACCCTCCAGGACCTGGTGGTCCACACCCTGGCCATGGCCCAGGACACTGTCCTGGAGGACATCACCC
TCCAGGACATGGTGGCCCATCCCATGGCCATGGCCCAGGGCACTGTCCTGGAGGACATCACCCTCCAGGA
CATGGTGGCCCATCCCATGGCCATGGCCCAGGGCACTGTGGCCCTCACCCTGGTCCACATCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200233 protein sequence
Red=Cloning site Green=Tags(s)

MHPGGKGHCGPPPGHGPGHCHEGHHPPGPGGPHPGHGPGHCPGGHHPPGHGGPSHGHGPGHCPGGHHPPG
HGGPSHGHGPGHCGPHPGPHH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC031454
ORF Size 273 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031454, AAH31454
RefSeq Size 539 bp
RefSeq ORF 275 bp
Locus ID 53906
Cytogenetics 2 59.46 cM
MW 8.7 kDa
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"BC031454" in other vectors (4)
SKU Description Size Price
MC206875 1810007E14Rik (untagged) - Mouse RIKEN cDNA 1810007E14 gene (cDNA clone MGC:25888 IMAGE:4216482), (10ug) 10 ug
$165.00
MG200233 1810007E14Rik (tGFP-tagged) - Mouse RIKEN cDNA 1810007E14 gene (cDNA clone MGC:25888 IMAGE:4216482) 10 ug
$350.00
MR200233L3 Lenti ORF clone of 1810007E14Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810007E14 gene (cDNA clone MGC:25888 IMAGE:4216482) 10 ug
$450.00
MR200233L4 Lenti ORF clone of 1810007E14Rik (mGFP-tagged) - Mouse RIKEN cDNA 1810007E14 gene (cDNA clone MGC:25888 IMAGE:4216482) 10 ug
$450.00

Plasmid Map for MR200233

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200233
Circular map for MR200233

file-alt Citations

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