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Ikzf1 (NM_001025597) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG227516
Ikzf1 (tGFP-tagged) - Mouse IKAROS family zinc finger 1 (Ikzf1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $727.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ikzf1
Synonyms 5832432G11Rik; hlk-1; I; Ikaros; LyF-; LyF-1; mKIAA4227; Zfpn; Zfpn1a1; Znfn1a1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG227516 representing NM_001025597
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGTCGATGAGGGTCAAGACATGTCCCAAGTTTCAGGAAAGGAGAGCCCCCCAGTCAGTGACACTC
CAGATGAAGGGGATGAGCCCATGCCTGTCCCTGAGGACCTGTCCACTACCTCTGGAGCACAGCAGAACTC
CAAGAGTGATCGAGGCATGGCCAGTAATGTTAAAGTAGAGACTCAGAGTGATGAAGAGAATGGGCGTGCC
TGTGAAATGAATGGGGAAGAATGTGCAGAGGATTTACGAATGCTTGATGCCTCGGGAGAGAAAATGAATG
GCTCCCACAGGGACCAAGGCAGCTCGGCTTTGTCAGGAGTTGGAGGCATTCGACTTCCTAACGGAAAACT
AAAGTGTGATATCTGTGGGATCGTTTGCATCGGGCCCAATGTGCTCATGGTTCACAAAAGAAGTCATACT
GGTGAACGGCCTTTCCAGTGCAACCAGTGTGGGGCCTCCTTTACCCAGAAAGGCAACCTCCTGCGGCACA
TCAAGCTGCACTCGGGTGAGAAGCCCTTCAAATGCCATCTTTGCAACTATGCCTGCCGCCGGAGGGACGC
CCTCACCGGCCACCTGAGGACGCACTCCGTTGGTAAGCCTCACAAATGTGGATATTGTGGCCGGAGCTAT
AAACAGCGAAGCTCTTTAGAGGAGCATAAAGAGCGATGCCACAACTACTTGGAAAGCATGGGCCTTCCGG
GCATGTACCCAGTCATTAAGGAAGAAACTAACCACAACGAGATGGCAGAAGACCTGTGCAAGATAGGAGC
AGAGAGGTCCCTTGTCCTGGACAGGCTGGCAAGCAATGTCGCCAAACGTAAGAGCTCTATGCCTCAGAAA
TTTCTTGGAGACAAGTGCCTGTCAGACATGCCCTATGACAGTGCCAACTATGAGAAGGAGGATATGATGA
CATCCCACGTGATGGACCAGGCCATCAACAATGCCATCAACTACCTGGGGGCTGAGTCCCTGCGCCCATT
GGTGCAGACACCCCCCGGTAGCTCCGAGGTGGTGCCAGTCATCAGCTCCATGTACCAGCTGCACAAGCCC
CCCTCAGATGGCCCCCCACGGTCCAACCATTCAGCACAGGACGCCGTGGATAACTTGCTGCTGCTGTCCA
AGGCCAAGTCTGTGTCATCGGAGCGAGAGGCCTCCCCGAGCAACAGCTGCCAAGACTCCACAGATACAGA
GAGCAACGCGGAGGAACAGCGCAGCGGCCTTATCTACCTAACCAACCACATCAACCCGCATGCACGCAAT
GGGCTGGCTCTCAAGGAGGAGCAGCGCGCCTACGAGGTGCTGAGGGCGGCCTCAGAGAACTCGCAGGATG
CCTTCCGTGTGGTCAGCACGAGTGGCGAGCAGCTGAAGGTGTACAAGTGCGAACACTGCCGCGTGCTCTT
CCTGGATCACGTCATGTATACCATTCACATGGGCTGCCATGGCTTTCGGGATCCCTTTGAGTGTAACATG
TGTGGTTATCACAGCCAGGACAGGTACGAGTTCTCATCCCATATCACGCGGGGGGAGCATCGTTACCACC
TGAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG227516 representing NM_001025597
Red=Cloning site Green=Tags(s)

MDVDEGQDMSQVSGKESPPVSDTPDEGDEPMPVPEDLSTTSGAQQNSKSDRGMASNVKVETQSDEENGRA
CEMNGEECAEDLRMLDASGEKMNGSHRDQGSSALSGVGGIRLPNGKLKCDICGIVCIGPNVLMVHKRSHT
GERPFQCNQCGASFTQKGNLLRHIKLHSGEKPFKCHLCNYACRRRDALTGHLRTHSVGKPHKCGYCGRSY
KQRSSLEEHKERCHNYLESMGLPGMYPVIKEETNHNEMAEDLCKIGAERSLVLDRLASNVAKRKSSMPQK
FLGDKCLSDMPYDSANYEKEDMMTSHVMDQAINNAINYLGAESLRPLVQTPPGSSEVVPVISSMYQLHKP
PSDGPPRSNHSAQDAVDNLLLLSKAKSVSSEREASPSNSCQDSTDTESNAEEQRSGLIYLTNHINPHARN
GLALKEEQRAYEVLRAASENSQDAFRVVSTSGEQLKVYKCEHCRVLFLDHVMYTIHMGCHGFRDPFECNM
CGYHSQDRYEFSSHITRGEHRYHLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001025597
ORF Size 1545 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001025597.2
RefSeq Size 5163 bp
RefSeq ORF 1548 bp
Locus ID 22778
Cytogenetics 11 7.02 cM
Summary The protein encoded by this gene belongs to a family of transcription factors that are characterized by a set of four DNA-binding zinc fingers at the N-terminus and two C-terminal zinc fingers involved in protein dimerization. It is regulated by both epigenetic and transcription factors. This protein is a transcriptional regulator of hematopoietic cell development and homeostasis. In addition, it is required to confer temporal competence to retinal progenitor cells during embryogenesis, demonstrating an essential function in nervous system development. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2014
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Documents
Resources
"NM_001025597" in other vectors (4)
SKU Description Size Price
MC217296 Ikzf1 (untagged) - Mouse IKAROS family zinc finger 1 (Ikzf1), transcript variant 1, (10ug) 10 ug
$480.00
MR227516 Ikzf1 (Myc-DDK-tagged) - Mouse IKAROS family zinc finger 1 (Ikzf1), transcript variant 1 10 ug
$289.00 $527.00
MR227516L3 Lenti ORF clone of Ikzf1 (Myc-DDK-tagged) - Mouse IKAROS family zinc finger 1 (Ikzf1), transcript variant 1 10 ug
$827.00
MR227516L4 Lenti ORF clone of Ikzf1 (mGFP-tagged) - Mouse IKAROS family zinc finger 1 (Ikzf1), transcript variant 1 10 ug
$827.00

Plasmid Map for MG227516

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG227516
Circular map for MG227516

file-alt Citations

Regulatory Phosphorylation of Ikaros by Bruton's Tyrosine Kinase
Ma, Qazi, Ozer et al
PLoS ONE (2013) 8 (8), e71302 DOI: 10.1371/journal.pone.0071302
Cited by 20
AI Summary: OriGene’s monoclonal mouse anti-DDK antibody (TA50011–100) was used in this study to detect DDK-tagged Ikaros proteins, enabling the analysis of BTK-dependent phosphorylation and its effects on Ikaros function.
Snippet: and BTK-phosphorylation site mutant IK proteins, cells were incubated with a monoclonal mouse anti-DDK (TA50011–100, Origene) and a ... The positions of the autophosphorylated BTK kinase band and BTK kinase domain band as well as the phosphorylated recombinant IK
FigureFigureFigure
WB
Showing 1-1 of 1 papers.
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