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Ctsb (NM_007798) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG227342
Ctsb (tGFP-tagged) - Mouse cathepsin B (Ctsb), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctsb
Synonyms CB
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG227342 representing NM_007798
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTGGTCCTTGATCCTTCTTTCTTGCCTGCTGGCACTGACCAGTGCCCATGACAAGCCTTCCTTCC
ACCCGCTGTCGGATGACCTGATTAACTATATCAACAAACAGAATACAACATGGCAGGCTGGACGCAACTT
CTACAATGTTGACATAAGCTATCTGAAGAAGCTGTGTGGCACTGTCCTGGGTGGACCCAAACTGCCAGGA
AGGGTTGCGTTCGGTGAGGACATAGATCTACCTGAAACCTTTGATGCACGGGAACAATGGTCCAACTGCC
CGACCATTGGACAGATTAGAGACCAGGGCTCCTGCGGCTCTTGTTGGGCATTTGGGGCAGTGGAAGCCAT
TTCTGACCGAACCTGCATTCACACCAATGGCCGAGTCAACGTGGAGGTGTCTGCTGAAGACCTGCTTACT
TGCTGTGGTATCCAGTGTGGGGACGGCTGTAATGGTGGCTATCCCTCTGGAGCATGGAGCTTCTGGACAA
AAAAAGGCCTGGTTTCAGGTGGAGTCTACAATTCTCATGTAGGCTGCTTACCATACACCATCCCTCCCTG
CGAGCACCATGTCAATGGCTCCCGTCCCCCATGCACTGGAGAAGGAGATACTCCCAGGTGCAACAAGAGC
TGTGAAGCTGGCTACTCCCCATCCTACAAAGAGGATAAGCACTTTGGGTACACTTCCTACAGCGTGTCTA
ACAGTGTGAAGGAGATCATGGCAGAAATCTACAAAAATGGCCCAGTGGAGGGTGCCTTCACTGTGTTTTC
TGACTTCTTGACTTACAAATCAGGAGTATACAAGCATGAAGCCGGTGATATGATGGGTGGCCACGCCATC
CGCATCCTGGGCTGGGGAGTAGAGAATGGAGTTCCCTACTGGCTGGCAGCCAACTCTTGGAACCTTGACT
GGGGTGATAATGGCTTCTTTAAAATCCTCAGAGGAGAGAACCACTGTGGCATTGAATCAGAAATTGTGGC
TGGAATCCCACGCACTGACCAGTACTGGGGAAGATTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG227342 representing NM_007798
Red=Cloning site Green=Tags(s)

MWWSLILLSCLLALTSAHDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPG
RVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLT
CCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKS
CEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAI
RILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007798
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007798.3, NP_031824.1
RefSeq Size 4756 bp
RefSeq ORF 1020 bp
Locus ID 13030
UniProt ID P10605
Cytogenetics 14 33.24 cM
Summary This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. provided by RefSeq, Aug 2015
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"NM_007798" in other vectors (3)
SKU Description Size Price
MR227342 Ctsb (Myc-DDK-tagged) - Mouse cathepsin B (Ctsb) 10 ug
$289.00 $457.00
MR227342L3 Lenti ORF clone of Ctsb (Myc-DDK-tagged) - Mouse cathepsin B (Ctsb) 10 ug
$757.00
MR227342L4 Lenti ORF clone of Ctsb (mGFP-tagged) - Mouse cathepsin B (Ctsb) 10 ug
$757.00

Plasmid Map for MG227342

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG227342
Circular map for MG227342

file-alt Citations

S-nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy
Qian, Zhang, Orwig et al
Diabetes (2017)
AI Summary: OriGene contributed to this study by providing the cathepsin B (CTSB) construct, which was used to investigate the role of lysosomal enzymes in autophagy and insulin resistance.
Snippet: Mutagenesis, adenovirus and adeno-associated virus transduction: The hexosaminidase subunit beta (HexB) and cathepsin B (CTSB) constructs were purchased from Sino Biological Inc and OriGene respectively, and the S- ...;... Mutagenesis, adenovirus and adeno-associated virus transduction: The hexosaminidase subunit beta (HexB) and cathepsin B (CTSB) constructs were purchased from Sino Biological Inc and OriGene respectively, and the S
Showing 1-1 of 1 papers.
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