Src (NM_001025395) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG227252
Src (tGFP-tagged) - Mouse Rous sarcoma oncogene (Src) transcript variant 2, (10ug)
$997.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Src |
| Synonyms | AW259666; pp60c-src |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001025395 |
| ORF Size | 1605 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001025395.2, NP_001020566.1 |
| RefSeq Size | 3869 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 20779 |
| UniProt ID | P05480 |
| Cytogenetics | 2 78.35 cM |
| Summary | Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN) (By similarity). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors (PubMed:9344858). Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1. Plays a role in EGF-mediated calcium-activated chloride channel activation (By similarity). Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus (By similarity). Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function (PubMed:14739300). Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase (PubMed:12615910). Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-738'. Enhances DDX58/RIG-I-elicited antiviral signaling. Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-226'. Phosphorylates CBLC |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001025395" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC217579 | Src (untagged) - Mouse Rous sarcoma oncogene (Src), transcript variant 2, (10ug) | 10 ug |
$748.00
|
|
| MR227252 | Src (Myc-DDK-tagged) - Mouse Rous sarcoma oncogene (Src), transcript variant 2 | 10 ug |
$797.00
|
|
| MR227252L3 | Lenti ORF clone of Src (Myc-DDK-tagged) - Mouse Rous sarcoma oncogene (Src), transcript variant 2 | 10 ug |
$1,097.00
|
|
| MR227252L4 | Lenti ORF clone of Src (mGFP-tagged) - Mouse Rous sarcoma oncogene (Src), transcript variant 2 | 10 ug |
$1,097.00
|
|
Citations
Group V Secretory Phospholipase A2 Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line
Fujioka, Watanabe, Nakamura et al
J Atheroscler Thromb (2021)
Fujioka, Watanabe, Nakamura et al
J Atheroscler Thromb (2021)
Cited by 2
AI Summary: OriGene products contributed to this study by providing the mouse PGK1 expression vector (accession no. NM_008828) and mouse c-Src expression vector (accession no. NM_011110.4), which were used to investigate the molecular mechanisms underlying sPLA2-V-regulated endocytosis in RAW264.7 macrophage cells.
Snippet: NM_011110.4) was purchased from OriGene Technologies (Rockville, MD, USA) A mouse PGK1 expression vector (accession no. NM_008828) was purchased from Origene Technologies. A mouse c-Src expression vector (accession no;- NM_011110.4) was purchased from OriGene Technologies (Rockville, MD, USA) A mouse PGK1 expression vector (accession no. NM_008828) was purchased from Origene Technologies. A mouse c-Src expression vector (accession no
Girdin Phosphorylation Is Crucial for Synaptic Plasticity and Memory: A Potential Role in the Interaction of BDNF/TrkB/Akt Signaling with NMDA Receptor
Nakai, Nagai, Tanaka et al
J. Neurosci. (2014) 34 (45), 14995-5008 DOI: 10.1523/JNEUROSCI.2228-14.2014
Nakai, Nagai, Tanaka et al
J. Neurosci. (2014) 34 (45), 14995-5008 DOI: 10.1523/JNEUROSCI.2228-14.2014
Showing 1-2 of 2 papers.
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