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Nfe2l2 (NM_010902) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG226717
Nfe2l2 (tGFP-tagged) - Mouse nuclear factor erythroid derived 2 like 2 (Nfe2l2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $1,033.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nfe2l2
Synonyms Nr; Nrf2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226717 representing NM_010902
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGACTTGGAGTTGCCACCGCCAGGACTACAGTCCCAGCAGGACATGGATTTGATTGACATCCTTT
GGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGA
GCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTT
TTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGA
CAGACACCAGTGGATCCGCCAGCTACTCCCAGGTTGCCCACATTCCCAAACAAGATGCCTTGTACTTTGA
AGACTGTATGCAGCTTTTGGCAGAGACATTCCCATTTGTAGATGACCATGAGTCGCTTGCCCTGGATATC
CCCAGCCACGCTGAAAGTTCAGTCTTCACTGCCCCTCATCAGGCCCAGTCCCTCAATAGCTCTCTGGAGG
CAGCCATGACTGATTTAAGCAGCATAGAGCAGGACATGGAGCAAGTTTGGCAGGAGCTATTTTCCATTCC
CGAATTACAGTGTCTTAATACCGAAAACAAGCAGCTGGCTGATACTACCGCTGTTCCCAGCCCAGAAGCC
ACACTGACAGAAATGGACAGCAATTACCATTTTTACTCATCGATCTCCTCGCTGGAAAAAGAAGTGGGCA
ACTGTGGTCCACATTTCCTTCATGGTTTTGAGGATTCTTTCAGCAGCATCCTCTCCACTGATGATGCCAG
CCAGCTGACCTCCTTAGACTCAAATCCCACCTTAAACACAGATTTTGGCGATGAATTTTATTCTGCTTTC
ATAGCAGAGCCCAGTGACGGTGGCAGCATGCCTTCCTCCGCTGCCATCAGTCAGTCACTCTCTGAACTCC
TGGACGGGACTATTGAAGGCTGTGACCTGTCACTGTGTAAAGCTTTCAACCCGAAGCACGCTGAAGGCAC
AATGGAATTCAATGACTCTGACTCTGGCATTTCACTGAACACGAGTCCCAGCCGAGCGTCCCCAGAGCAC
TCCGTGGAGTCTTCCATTTACGGAGACCCACCGCCTGGGTTCAGTGACTCGGAAATGGAGGAGCTAGATA
GTGCCCCTGGAAGTGTCAAACAGAACGGCCCTAAAGCACAGCCAGCACATTCTCCTGGAGACACAGTACA
GCCTCTGTCACCAGCTCAAGGGCACAGTGCTCCTATGCGTGAATCCCAATGTGAAAATACAACAAAAAAA
GAAGTTCCCGTGAGTCCTGGTCATCAAAAAGCCCCATTCACAAAAGACAAACATTCAAGCCGCTTAGAGG
CTCATCTCACACGAGATGAGCTTAGGGCAAAAGCTCTCCATATTCCATTCCCTGTCGAAAAAATCATTAA
CCTCCCTGTTGATGACTTCAATGAAATGATGTCCAAGGAGCAATTCAATGAAGCTCAGCTCGCATTGATC
CGAGATATACGCAGGAGAGGTAAGAATAAAGTCGCCGCCCAGAACTGTAGGAAAAGGAAGCTGGAGAACA
TTGTCGAGCTGGAGCAAGACTTGGGCCACTTAAAAGACGAGAGAGAAAAACTACTCAGAGAAAAGGGAGA
AAACGACAGAAACCTCCATCTACTGAAAAGGCGGCTCAGCACCTTGTATCTTGAAGTCTTCAGCATGTTA
CGTGATGAGGATGGAAAGCCTTACTCTCCCAGTGAATACTCTCTGCAGCAAACCAGAGATGGCAATGTGT
TCCTTGTTCCCAAAAGCAAGAAGCCAGATACAAAGAAAAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226717 representing NM_010902
Red=Cloning site Green=Tags(s)

MMDLELPPPGLQSQQDMDLIDILWRQDIDLGVSREVFDFSQRQKDYELEKQKKLEKERQEQLQKEQEKAF
FAQFQLDEETGEFLPIQPAQHIQTDTSGSASYSQVAHIPKQDALYFEDCMQLLAETFPFVDDHESLALDI
PSHAESSVFTAPHQAQSLNSSLEAAMTDLSSIEQDMEQVWQELFSIPELQCLNTENKQLADTTAVPSPEA
TLTEMDSNYHFYSSISSLEKEVGNCGPHFLHGFEDSFSSILSTDDASQLTSLDSNPTLNTDFGDEFYSAF
IAEPSDGGSMPSSAAISQSLSELLDGTIEGCDLSLCKAFNPKHAEGTMEFNDSDSGISLNTSPSRASPEH
SVESSIYGDPPPGFSDSEMEELDSAPGSVKQNGPKAQPAHSPGDTVQPLSPAQGHSAPMRESQCENTTKK
EVPVSPGHQKAPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVDDFNEMMSKEQFNEAQLALI
RDIRRRGKNKVAAQNCRKRKLENIVELEQDLGHLKDEREKLLREKGENDRNLHLLKRRLSTLYLEVFSML
RDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDTKKN

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010902
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010902.4
RefSeq Size 2469 bp
RefSeq ORF 1794 bp
Locus ID 18024
UniProt ID Q60795
Cytogenetics 2 44.75 cM
Summary This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_010902" in other vectors (6)
SKU Description Size Price
MC219509 Nfe2l2 (untagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2), (10ug) 10 ug
$835.00
MR226717 Nfe2l2 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2) 10 ug
$289.00 $833.00
MR226717L1 Lenti ORF clone of Nfe2l2 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2) 10 ug
$1,133.00
MR226717L2 Lenti ORF clone of Nfe2l2 (mGFP-tagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2) 10 ug
$1,133.00
MR226717L3 Lenti ORF clone of Nfe2l2 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2) 10 ug
$1,133.00
MR226717L4 Lenti ORF clone of Nfe2l2 (mGFP-tagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2) 10 ug
$1,133.00

Plasmid Map for MG226717

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG226717
Circular map for MG226717

file-alt Citations

Isoniazid induces apoptosis: Role of oxidative stress and inhibition of nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2)
Verma, Yadav, Singh et al
Life Sci. (2018) 199, 23-33 DOI: 10.1016/j.lfs.2018.02.037
AI Summary: In this study, the pCMV6-AC-GFP Nrf2 construct from OriGene was used to overexpress Nrf2 in HepG2 cells, helping to investigate the role of Nrf2 in isoniazid-induced apoptosis and oxidative stress.
Snippet: Healthcare). 2.12. Plasmid transfection. HepG2 cells were seeded and were grown for 24 h. Then HepG2 cells were transfected with pCMV6-AC-GFP Nrf2 construct containing Nfr2 ORF (Origene) using Exgen 500 (Fermentas)
Contribution of Nrf2 to Atherogenic Phenotype Switching of Coronary Arterial Smooth Muscle Cells Lacking CD38 Gene
Xu, Li, Wang et al
Cell. Physiol. Biochem. (2015) 37 (2), 432-44 DOI: 10.1159/000430366
Cited by 33
AI Summary: OriGene products contributed to this study by providing CD38 cDNA (Catalog# MG204265) and Nrf2 cDNA plasmids (Catalog# MG226717), which were used for gene overexpression experiments to investigate the molecular mechanisms underlying phenotypic switching in coronary arterial smooth muscle cells.
Snippet: The results were expressed as the fold change of the treatment group versus the control. Nucleofection of CD38 cDNA and Nrf2 cDNA. Both CD38 cDNA (Catalog# MG204265) and Nrf2 cDNA plasmids (Catalog# MG226717) were purchased from OriGene Technologies ... Cite SaveSaving
Showing 1-2 of 2 papers.
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