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Hes5 (NM_010419) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG226205
Hes5 (tGFP-tagged) - Mouse hairy and enhancer of split 5 (Drosophila) (Hes5), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hes5
Synonyms bHLHb38
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226205 representing NM_010419
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCAAGTACCGTGGCGGTGGAGATGCTCAGTCCCAAGGAGAAAAACCGACTGCGGAAGCCGGTGG
TGGAGAAGATGCGTCGGGACCGCATCAACAGCAGCATAGAGCAGCTGAAGCTGCTGCTGGAGCAGGAGTT
CGCGCGGCACCAGCCCAACTCCAAGCTGGAGAAGGCCGACATCCTGGAGATGGCCGTCAGCTACCTGAAA
CACAGCAAAGCCTTCGCCGCGGCCGCCGGCCCCAAGAGCCTGCACCAGGACTACAGCGAGGGCTACTCCT
GGTGCCTGCAGGAGGCGGTACAGTTCCTGACCCTGCACGCCGCCAGCGACACGCAGATGAAGCTGCTTTA
CCACTTCCAGCGGCCCCCAGCTCCCGCCGCTCCTGCCAAGGAGCCCCCGGCGCCCGGAGCTGCGCCCCAA
CCGGCCCGCTCCTCCGCTAAGGCTGCTGCTGCTGCGGTCTCCACCTCGCGCCAACCCGCCTGCGGCCTCT
GGCGGCCCTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226205 representing NM_010419
Red=Cloning site Green=Tags(s)

MAPSTVAVEMLSPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLK
HSKAFAAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAPAAPAKEPPAPGAAPQ
PARSSAKAAAAAVSTSRQPACGLWRPW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010419
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010419.4, NP_034549.1
RefSeq Size 1273 bp
RefSeq ORF 504 bp
Locus ID 15208
UniProt ID P70120
Cytogenetics 4 86.01 cM
Summary Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.UniProtKB/Swiss-Prot Function
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"NM_010419" in other vectors (4)
SKU Description Size Price
MC208659 Hes5 (untagged) - Mouse hairy and enhancer of split 5 (Drosophila) (Hes5), (10ug) 10 ug
$330.00
MR226205 Hes5 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 5 (Drosophila) (Hes5) 10 ug
$289.00 $300.00
MR226205L3 Lenti ORF clone of Hes5 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 5 (Drosophila) (Hes5) 10 ug
$600.00
MR226205L4 Lenti ORF clone of Hes5 (mGFP-tagged) - Mouse hairy and enhancer of split 5 (Drosophila) (Hes5) 10 ug
$600.00

Plasmid Map for MG226205

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG226205
Circular map for MG226205

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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