Traf3 (NM_001048206) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG225762
Traf3 (tGFP-tagged) - Mouse TNF receptor-associated factor 3 (Traf3) transcript variant 2, (10ug)
  $957.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Traf3
Synonyms AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1; T-BAM
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG225762 representing NM_001048206
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAAGCAAAAAGATGGATGCTGCTGGCACACTGCAGCCTAACCCACCCCTAAAGCTGCAGCCTG
ATCGCGGCGCAGGGTCCGTGCTCGTGCCGGAGCAAGGAGGCTACAAGGAGAAGTTTGTGAAGACGGTGGA
AGACAAGTACAAGTGCGAGAAGTGCCGCCTGGTGCTGTGCAACCCGAAGCAGACGGAGTGTGGCCACCGG
TTCTGCGAGAGCTGCATGGCCGCCCTGCTGAGCTCCTCCAGTCCAAAATGCACAGCGTGCCAAGAAAGCA
TCATCAAAGACAAGGTGTTTAAGGATAATTGCTGCAAGAGAGAGATTCTGGCCCTTCAGGTCTACTGTCG
GAATGAAGGCAGAGGTTGTGCGGAGCAGCTGACTCTGGGACATCTGCTGGTGCACCTAAAAAATGAATGT
CAGTTTGAGGAACTTCCCTGTCTGCGTGCCGACTGCAAAGAAAAAGTACTGAGAAAAGACTTGCGGGATC
ACGTGGAAAAGGCCTGTAAATACCGCGAGGCCACGTGCAGTCACTGCAAGAGCCAAGTGCCCATGATCAA
ACTGCAGAAACATGAAGACACAGATTGTCCCTGTGTGGTGGTATCCTGCCCTCACAAGTGCAGCGTTCAG
ACTCTTCTAAGGAGTGAGGGTACAAACCAGCAGATCAAGGCCCATGAGGCCAGCTCCGCGGTACAGCACG
TGAACCTGCTGAAGGAGTGGAGCAACTCCCTGGAGAAGAAGGTTTCCCTGCTGCAGAATGAAAGTGTTGA
GAAAAACAAGAGCATCCAAAGCCTGCACAACCAGATCTGCAGCTTTGAGATCGAGATTGAGAGGCAGAAG
GAGATGCTCCGAAACAACGAGTCCAAGATCCTTCACCTGCAGCGGGTAATCGACAGCCAAGCAGAGAAAC
TGAAAGAACTGGACAAGGAGATCCGTCCCTTCCGGCAGAACTGGGAGGAAGCGGACAGCATGAAGAGCAG
TGTGGAGTCCCTCCAGAACCGAGTGACTGAGCTGGAGAGCGTAGACAAAAGTGCGGGGCAGGCGGCTCGC
AACACAGGCTTGCTGGAGTCCCAGCTGAGCCGGCATGACCAGATGTTGAGTGTTCATGACATCCGCTTGG
CCGACATGGACCTGCGGTTCCAGGTCCTCGAGACCGCCAGCTACAACGGGGTGCTGATCTGGAAGATCCG
TGACTACAAGCGCCGGAAGCAGGAGGCCGTCATGGGGAAGACCCTGTCTCTCTACAGCCAGCCTTTCTAC
ACAGGTTATTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAATGGGGACGGAATGGGGAAAGGGACAC
ACTTGTCGCTGTTTTTTGTCATTATGCGTGGAGAATATGATGCTCTGTTGCCATGGCCGTTCAAGCAGAA
AGTGACACTTATGCTGATGGATCAGGGGTCCTCTCGCCGTCATCTGGGAGATGCGTTCAAGCCTGACCCC
AACAGCAGCAGCTTCAAGAAACCCACCGGAGAGATGAATATCGCCTCTGGCTGCCCAGTCTTTGTCGCCC
AAACTGTTCTAGAGAACGGGACGTATATTAAAGATGATACAATCTTTATTAAGGTCATAGTGGATACCTC
GGATCTGCCTGACCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG225762 representing NM_001048206
Red=Cloning site Green=Tags(s)

MESSKKMDAAGTLQPNPPLKLQPDRGAGSVLVPEQGGYKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHR
FCESCMAALLSSSSPKCTACQESIIKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNEC
QFEELPCLRADCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIKLQKHEDTDCPCVVVSCPHKCSVQ
TLLRSEGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQK
EMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQAAR
NTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFY
TGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDP
NSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001048206
ORF Size 1626 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001048206.1, NP_001041671.1
RefSeq Size 6906 bp
RefSeq ORF 1629 bp
Locus ID 22031
Cytogenetics 12 60.94 cM
Summary Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001048206" in other vectors (4)
SKU Description Size Price
MC218259 Traf3 (untagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2, (10ug) 10 ug
$808.00
MR225762 Traf3 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 10 ug
$757.00
MR225762L3 Lenti ORF clone of Traf3 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 10 ug
$1,057.00
MR225762L4 Lenti ORF clone of Traf3 (mGFP-tagged) - Mouse TNF receptor-associated factor 3 (Traf3), transcript variant 2 10 ug
$1,057.00

Plasmid Map for MG225762

Download sequence for

Cloning scheme for MG225762

Circular map for MG225762

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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