Rictor (NM_030168) Mouse Tagged ORF Clone

SKU MG225754

Rictor (tGFP-tagged) - Mouse RPTOR independent companion of MTOR complex 2 (Rictor), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Rictor
• Synonyms: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225754 representing NM_030168; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAATACGAGGGCGGAATGACAGCGGCGAGG; AGAACGTCCCGCTCGATCTGACCCGAGAACCTTCTGATAACTTGAGGGAGATTCTCCAAAATGTGGCCAA; ATTGCAAGGAGTATCAAATATGAGAAAGCTGGGCCATCTGAATAACTTCACTAAGCTTCTTTGCGATATT; GGCCATAGTGAAGAAAAACTGGGCTTTAATTATGAAGACATCATAATTTGTTTGCGCTTAGCTTTATTAA; ATGAGGCAAAAGAAGTAAGAGCAGCCGGGTTGCGAGCGCTGCGCTATCTCATCCAAGATTCCAGTATTCT; CCAGAAGGTGCTAAAACTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATTCAGCAGAGCAACGAG; GTGGAGAGGACACAAGCCCTTCGCTTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTT; CTGTGGCCAACTCATTAATTGCGGTTGGAAATGATGGGCTTCAGGAACGAGACAGAATGGTCCGGGCTTG; CATCGCCATCATCTGTGAACTAGCACTTCAGAACCCAGAGGTGGTAGCCCTTCGAGGAGGACTAAACACC; ATACTGAAAAATGTGATTGATTGCCAATTAAGTCGAATAAATGAGGCTCTCATTACTACAATTTTGCACC; TTCTTAATCATCCAAAGACCCGGCAGTATGTGCGAGCTGATGTAGAATTAGAGCGAATTTTAGCACCCTA; TACTGATTTCCACTACAGACACAGTCCAGATACGGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA; TTTCTAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGC; CTGGAAATTCTGGGATACAGTCTCTAATTGGAGTACTTTGCATACCGAATATGGAAATAAGGCGAGGTCT; GCTCGAAGTGCTCTATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAAGCCCTG; CTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTCCGATGGCTTTGTGGCAGCTGAGGCAA; AAACTATTCTTCCCCATCGTGCTAGATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTC; TGCATTTATTCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCA; GTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATGGCAAACACCATCCTGCCTCACTCGCATAGCC; ACCATCTACACTGCTTGCCAACCCTCATGAACATGGCTGCGTCCTTCGATATTCCCAAGGAAAAGAGGCT; GCGAGCCAGTGCAGCCCTGAACTGTTTGAACCGCTTCCATGAGATGAAGAAGCGAGGACCTAAGCCCTAC; AGCCTTCATTTAGATCACATCATTCAGAAAGCAATCGCAACTCACCACAAGCGGGATCAGTATCTTCGAG; TTCAGAAAGATATATTTGTTCTTAAGGATACAGAGGAAGCTCTTTTAATAAACCTTAGAGACAGCCAAGT; CCTTCAGCATAAAGAGAATCTTGACTGGGATTGGAATCTGATTGGGACCATCCTTAAGTGGCCAAATGTA; AATCTAAGAAACTATAAAGATGAGCAGTTGCACAGGTTTGTGCGCAGACTTCTTTACTTTTACAAGCCCA; GCAGCAAACTGTACGCTAGTCTGGATCTGGACTTGGCCAAGTCCAAGCAGCTCACAGTTGTTGGTTGTCA; GTTTACAGAATTTCTGCTCGAGTCTGAAGAGGATGGGCAAGGATACTTAGAAGATCTCGTGAAAGATATT; GTTCAGTGGCTCAATGCTTCATCTGGAGTGAAACCTGAGAGAAGCCTTCAGAATAATGGCTTACTGACCA; CCCTTAGTCAACACTACTTCTTATTTATCGGAACACTCTCTTGTCATCCTCATGGAGTCAAAATGCTGGA; AAAGTGCAGTGTCTTTCAGTGTCTCCTTAATCTTTGTTCCTTGAAAAACCAAGATCATCTGATAAAACTC; ACTGTTTCCAGCCTAGACTATAGCAGAGATGGATTAGCCAGAGTCATTCTTTCAAAAATCCTCACGGCAG; CCACAGATGCTTGCAGACTGTATGCAACAAAACATTTAAGGGTTTTATTGAGAGCTAACGTTGAATTCTT; CAACAATTGGGGAATTGAGTTACTAGTAACTCAGCTACATGATAAAAACAAAACAATTTCTTCTGAAGCG; CTGGATATTCTTGATGAAGCTTGTGAAGACAAGGCCAATCTTCATGCTCTTATTCAGATGAAGCCAGCCT; TATCCCACCTTGGAGACAAGGGCTTGCTTCTACTGCTGAGATTTCTTTCCATTCCGAAAGGATTTTCTTA; CCTGAATGAAAGGGGTTATGTTGCAAAACAGTTGGAAAAGTGGCACAAGGAATATAATTCAAAGTATGTT; GACTTGATTGAGGAACAACTTAATGAAGCCCTCACTACATACAGGAAGCCTATTGATGGTGATAACTACG; TTCGTCGCAGTAATCAAAGACTACAGCGACCTCATGTCTACCTGCCCGTACACCTTTATGGACAACTGGT; ACACCACAAAACAGGCTGCCATTTACTGGAAGTACAGAGTATCATCACAGAGCTCTGTCACAATGTTCGC; ACTCCAGACTTGGACAAATGGGAAGACATTAAGAAGCTGAAAGCCTCTCTTTGGGCCTTGGGAAATATTG; GCTCATCGAATTGGGGGCTTAATTTGCTACAGGAAGAAAATGTGATCCCAGATATACTAAAACTTGCAAA; ACAGTGTGAGGTCCTTTCCATCCGAGGGACCTGTGTATATGTTCTTGGGCTCATAGCTAAAACCAAGCAA; GGCTGTGATATTTTGAAATGTCACAGCTGGGATTCTGTAAGGCACAGTCGCAAGCATCTATGGCCAGTGG; TTCCAGATGATGTGGAGCAGCTCTGCAATGAGCTCTCATCTGTCCCAAGCACCCTCAGCTTGAACTCTGA; GTCGACCAGCTCTAGACATAACAGTGAGAGTGAGTCTGCACCATCAAGCATGTTCATGCTGGAGGACGAC; CGCTTCGGCAGCACCTCCACCAGCACATTTTTCCTTGACATCAATGAAGATGCTGAGCCAGCATTTTATG; ACCGACCTGGACCCATAAAAGATAAAAATTCATTTCCCTTTTTTGGCTCCAGCAAACTTGTAAAGAATCG; TATCTTAAATTCACTTACTTTGCCTACTAAAAAACACCGCAGTAGCAGTGATCCAAAAGGAGGAAAACTG; TCATCTGAAAATAAGACAAGCAACCGGCGAATCAGAACACTTACGGAGCCCAGTGTTGACTTAATCACAG; CGAAGACTTCACAAGCTCCTCAGCACAGAAATCATTACAGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225754 representing NM_030168; Red=Cloning site Green=Tags(s); ; MAAIGRGRSLKNLRIRGRNDSGEENVPLDLTREPSDNLREILQNVAKLQGVSNMRKLGHLNNFTKLLCDI; GHSEEKLGFNYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNE; VERTQALRLVRKMITVNASLFPSSVANSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNT; ILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREAR; FLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTDEFIEAL; LSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHIS; VRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLNRFHEMKKRGPKPY; SLHLDHIIQKAIATHHKRDQYLRVQKDIFVLKDTEEALLINLRDSQVLQHKENLDWDWNLIGTILKWPNV; NLRNYKDEQLHRFVRRLLYFYKPSSKLYASLDLDLAKSKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDI; VQWLNASSGVKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLIKL; TVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEA; LDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHKEYNSKYV; DLIEEQLNEALTTYRKPIDGDNYVRRSNQRLQRPHVYLPVHLYGQLVHHKTGCHLLEVQSIITELCHNVR; TPDLDKWEDIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQ; GCDILKCHSWDSVRHSRKHLWPVVPDDVEQLCNELSSVPSTLSLNSESTSSRHNSESESAPSSMFMLEDD; RFGSTSTSTFFLDINEDAEPAFYDRPGPIKDKNSFPFFGSSKLVKNRILNSLTLPTKKHRSSSDPKGGKL; SSENKTSNRRIRTLTEPSVDLITAKTSQAPQHRNHYS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030168
• ORF Size: 3471 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030168.3, NP_084444.3
• RefSeq Size: 9328 bp
• RefSeq ORF: 5127 bp
• Locus ID: 78757
• UniProt ID: Q6QI06
• Cytogenetics: 15 A1
• Summary: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.[UniProtKB/Swiss-Prot Function]