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Pde4d (NM_011056) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG225474
Pde4d (tGFP-tagged) - Mouse phosphodiesterase 4D cAMP specific (Pde4d), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $885.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde4d
Synonyms 9630011N22Rik; Dpde3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG225474 representing NM_011056
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGGGACACTTGTGATGTACTCTCTCGGAGCAAAAGTGCCTCTGAGGAAACACTGCACTCCTGTA
ATGAAGAAGAGGACCCTTTTCGTGGAATGGAGCCCTATCTCGTACGGCGACTTTCTTCCCGCAGCATTCA
GCTTCCACCTCTGGCCTTCAGACAGTTGGAACAAGCAGACTTGCGAAGCGAATCAGAGAACATCCCTCGG
CCAACTAGCCTCCCCTTGAAAATCCTGCCTCTGATCGCCGTCACATCTGCAGACTCAAGTGGTTTCGATG
TGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTGATTCT
CCAAGCAAACTTTGTCCACAGTCAACGCCGGGAGTCCTTCCTGTACCGATCTGACAGCGACTATGACCTC
TCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACCC
CGTTTGCTCAGGTCTTGGCTAGCCTGCGAACCGTACGGAACAACTTTGCTGCGTTAACGAATTTGCAAGA
CCGAGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAG
GCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGC
AGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAAAAGGATGCTGAACCGGGAGCTCACGCA
TCTCTCTGAGATGAGTCGGTCTGGCAACCAGGTGTCGGAGTACATCTCAAACACATTTCTCGATAAGCAA
CATGAAGTGGAAATCCCCTCTCCAACTCAGAAGGAAAAGGAGAAGAAGAAAAGGCCGATGTCGCAGATCA
GTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTAACTAATTCATGTATCCCCAGGTTTGGGGTTAAAAC
GGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATA
GCAGAGCTGTCTGGTAACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAA
AAACGTTTAAAATCCCAGTAGACACTTTGATTACGTATCTTATGACTCTGGAAGACCATTACCATGCTGA
CGTGGCCTATCACAACAACATCCATGCTGCAGACGTCGTCCAGTCCACTCACGTGCTGCTCTCTACACCC
GCTTTGGAGGCTGTGTTCACAGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGG
ACCATCCTGGGGTGTCAAATCAATTTCTGATCAATACAAACTCGGAACTCGCTCTGATGTACAACGACTC
CTCGGTCCTAGAGAACCATCACCTGGCGGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTC
CAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGGTCATTGACATTGTCCTGGCGACAGACA
TGTCGAAGCACATGAACCTGCTGGCTGATCTGAAAACCATGGTTGAAACCAAGAAGGTGACAAGCTCCGG
CGTCCTCCTCCTGGATAACTACTCCGACAGGATCCAGGTCCTTCAGAATATGGTGCACTGTGCAGACCTG
AGCAACCCCACAAAGCCACTCCAGCTGTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGCC
AGGGGGACCGAGAGCGGGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGA
AAAATCACAGGTGGGCTTCATAGACTATATCGTTCATCCACTCTGGGAGACGTGGGCAGACCTCGTACAT
CCCGATGCCCAGGACATCTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGA
GCCCCTCCCCTGCACCTGATGACCAAGAGGAGGGCCGGCAGGGACAGACTGAAAAATTCCAGTTTGAACT
AACCTTGGAGGAAGACTGTGAGTCAGACACTGAAAAGGACAGTGGAAGTCAGGTGGAGGAAGACACTAGC
TGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTG
AGGAGGAAGCTGTAGCAGAAGAGGAAAGCCAGCCTGAAACTTGCGTCCCAGATGACTGCTGTCCTGATAC
G

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG225474 representing NM_011056
Red=Cloning site Green=Tags(s)

MERDTCDVLSRSKSASEETLHSCNEEEDPFRGMEPYLVRRLSSRSIQLPPLAFRQLEQADLRSESENIPR
PTSLPLKILPLIAVTSADSSGFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDL
SPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEE
AYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQ
HEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRI
AELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTP
ALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIF
QNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADL
SNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVH
PDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEEGRQGQTEKFQFELTLEEDCESDTEKDSGSQVEEDTS
CSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPETCVPDDCCPDT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011056
ORF Size 2241 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011056.2, NP_035186.1
RefSeq Size 7049 bp
RefSeq ORF 2244 bp
Locus ID 238871
UniProt ID Q01063
Cytogenetics 13 59.69 cM
Summary Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.UniProtKB/Swiss-Prot Function
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"NM_011056" in other vectors (4)
SKU Description Size Price
MC221337 Pde4d (untagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d), (10ug) 10 ug
$753.00
MR225474 Pde4d (Myc-DDK-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d) 10 ug
$589.00 $685.00
MR225474L3 Lenti ORF clone of Pde4d (Myc-DDK-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d) 10 ug
$985.00
MR225474L4 Lenti ORF clone of Pde4d (mGFP-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d) 10 ug
$985.00

Plasmid Map for MG225474

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG225474
Circular map for MG225474

file-alt Citations

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