Il22 (NM_016971) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG225471
Il22 (tGFP-tagged) - Mouse interleukin 22 (Il22), (10ug)
  $650.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Il22
Synonyms IL-22; IL-22a; Iltif; ILTIFa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG225471 representing NM_016971
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCCTGCAGAAATCTATGAGTTTTTCCCTTATGGGGACTTTGGCCGCCAGCTGCCTGCTTCTCA
TTGCCCTGTGGGCCCAGGAGGCAAATGCGCTGCCCGTCAACACCCGGTGCAAGCTTGAGGTGTCCAACTT
CCAGCAGCCGTACATCGTCAACCGCACCTTTATGCTGGCCAAGGAGGCCAGCCTTGCAGATAACAACACA
GATGTCCGGCTCATCGGGGAGAAACTGTTCCGAGGAGTCAATGCTAAGGATCAGTGCTACCTGATGAAGC
AGGTGCTCAACTTCACCCTGGAAGACGTTCTGCTCCCCCAGTCAGACAGGTTCCAGCCCTACATGCAGGA
GGTGGTGCCTTTCCTGACCAAACTCAGCAATCAGCTCAGCTCCTGTCACATCAGCGGTGACGACCAGAAC
ATCCAGAAGAATGTCAGAAGGCTGAAGGAGACAGTGAAAAAGCTTGGAGAGAGTGGAGAGATCAAGGCGA
TTGGGGAACTGGACCTGCTGTTTATGTCTCTGAGAAATGCTTGCGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG225471 representing NM_016971
Red=Cloning site Green=Tags(s)

MAVLQKSMSFSLMGTLAASCLLLIALWAQEANALPVNTRCKLEVSNFQQPYIVNRTFMLAKEASLADNNT
DVRLIGEKLFRGVNAKDQCYLMKQVLNFTLEDVLLPQSDRFQPYMQEVVPFLTKLSNQLSSCHISGDDQN
IQKNVRRLKETVKKLGESGEIKAIGELDLLFMSLRNACV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016971
ORF Size 537 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016971.2, NP_058667.1
RefSeq Size 1088 bp
RefSeq ORF 540 bp
Locus ID 50929
UniProt ID Q9JJY9
Cytogenetics 10 66.67 cM
Summary Cytokine that contributes to the inflammatory response in vivo.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016971" in other vectors (4)
SKU Description Size Price
MC209879 Il22 (untagged) - Mouse interleukin 22 (Il22), (10ug) 10 ug
$300.00
MR225471 Il22 (Myc-DDK-tagged) - Mouse interleukin 22 (Il22) 10 ug
$450.00
MR225471L3 Lenti ORF clone of Il22 (Myc-DDK-tagged) - Mouse interleukin 22 (Il22) 10 ug
$750.00
MR225471L4 Lenti ORF clone of Il22 (mGFP-tagged) - Mouse interleukin 22 (Il22) 10 ug
$750.00

Plasmid Map for MG225471

Download sequence for

Cloning scheme for MG225471

Circular map for MG225471

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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