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Depdc1b (NM_178683) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG224877
Depdc1b (tGFP-tagged) - Mouse DEP domain containing 1B (Depdc1b), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $693.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Depdc1b
Synonyms 9830132O03; AW260467; XTP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG224877 representing NM_178683
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCACCGCGTAGTGGGACCCGGGCCGTACCGGGCCACCAGGCTGTGGAATGAAACAGTGGAGCTTT
TCCGGGCCAGGATGCCTTTACGGAGACATCGTTGCCGGTTCAAGAGTTACGAGCATTGCTTCACTGCATC
GGAGGCTGTGGACTGGCTGCACGAGCTTCTGCGGAACAGTCAAAACTTTGGGCCCGAGGTAACTCGCAAA
CAAACAGTCCAGCTGCTGGGAAAATTCCTGAAGAATCATGTTATTGAGGACATCAAGGGGAAGTGGGGCC
AGGAGGATTTTGAAGACAACCGTCACCTCTACAGATTTCCTCCCTCCTCACCCCTGAAGCCATATCCAAA
GAAGCCCCTGTATCAAAGAGATGTTATTAAGTTTCCAGAATGGAACGATCCCCCACCGGGCACTTCACAG
GAGAACATTCCAGTGAGGCCGATTGTGATGAATTCTGAGATGTGGTTCAAGCGCCACAGTATTGCTATTG
GAGAGGTGCCGGCATGCCGCCTCGTCCACCGCAGACAGCTGACCGAGGCCAATGTGGAAGAGATCTGGAA
GTCTACGACGTTGTCATATTTACAGAAAATTCTTGGCCTGGATTCCTTGGAGGAAGTTTTAAACACCAAG
CTGGTCAGCTCCAAGTTCATCATCCATAACGTGTACAGTGTCAGCAAGCAGGGAGTGGTCATCCTGGACG
ACAAGTCGAAAGAACTTCCTCACTGGGTCCTGTCAGCTATGAAGTGTTTGGCAAATTGGCCCAACTGTAC
AGACTGGAAGCAGCCTATGTACTTGGGATTTGAGAAGGATGTCTTTAAGACTATAGCAGATTACTATGGT
CACCTGAAGGAGCCACTGCTCACATTTCATCTCTTTGATGCTTTCGTCAGCGTCTTAGGTTTGCTGCCGA
AGGAGAAAACGGCCATTGAAGCATTTCAGCTTTGCTGTCTCCTCCTGCCTCCTGAAAACAGGAGAAAGTT
ACAGCTGTTGATGAGGATGATGGCGAGGATCTGCCTAAACAAGGAGATGCCCCCACTGAGTGATGGCTTC
GGCACCCGCACACTGATGGTTCAGACCTTTTCCCGGTGCATCCTGTGCTCTAAGGATGAAGTAGACTTAG
ATGAGTTACTAGCTGCTCGATTGGTGACATTCCTGATGGACAATTACCAGGAGATTCTGAAAGTGCCTCT
GGTCCTGCAGACCTCCATAGAAGAGCGTGTGGCTCATCTGCGAAGAGTCCAGATAAAATACCCAGGAGCT
GATATGGATATCACTCTGTCTGCACCTTCGTTTTGCCGTCAAATTAGTCCCGAGGAGTTTGAGTACCAAA
GGGCGTATGGCTCCCAGGAGCCCCTGGCAGCCTTGTTGGAAGAAGTCATAGCCGACGACAAACTCTCCAG
CAAGGAGAAGAAGAAGAAGCTGAAGCAGTTTCAGAAATCTTACCCTGAGGTCTACCAGGAACGATTTCCT
ACCCCAGAGAGCGAAGCACTTCTCTTTCCTGAAAAACCCAAGGCCAAGCCGCAGCTGTTCATGTGGGCAC
TGAGGAAGCCCTTCCAGCCGTTTCAGAGAACTAGAAGTTTCCGGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG224877 representing NM_178683
Red=Cloning site Green=Tags(s)

MEHRVVGPGPYRATRLWNETVELFRARMPLRRHRCRFKSYEHCFTASEAVDWLHELLRNSQNFGPEVTRK
QTVQLLGKFLKNHVIEDIKGKWGQEDFEDNRHLYRFPPSSPLKPYPKKPLYQRDVIKFPEWNDPPPGTSQ
ENIPVRPIVMNSEMWFKRHSIAIGEVPACRLVHRRQLTEANVEEIWKSTTLSYLQKILGLDSLEEVLNTK
LVSSKFIIHNVYSVSKQGVVILDDKSKELPHWVLSAMKCLANWPNCTDWKQPMYLGFEKDVFKTIADYYG
HLKEPLLTFHLFDAFVSVLGLLPKEKTAIEAFQLCCLLLPPENRRKLQLLMRMMARICLNKEMPPLSDGF
GTRTLMVQTFSRCILCSKDEVDLDELLAARLVTFLMDNYQEILKVPLVLQTSIEERVAHLRRVQIKYPGA
DMDITLSAPSFCRQISPEEFEYQRAYGSQEPLAALLEEVIADDKLSSKEKKKKLKQFQKSYPEVYQERFP
TPESEALLFPEKPKAKPQLFMWALRKPFQPFQRTRSFRM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178683
ORF Size 1587 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178683.4, NP_848798.1
RefSeq Size 2530 bp
RefSeq ORF 1590 bp
Locus ID 218581
UniProt ID Q8BH88
Cytogenetics 13 D2.1
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Resources
"NM_178683" in other vectors (4)
SKU Description Size Price
MC217642 Depdc1b (untagged) - Mouse DEP domain containing 1B (Depdc1b), (10ug) 10 ug
$543.00
MR224877 Depdc1b (Myc-DDK-tagged) - Mouse DEP domain containing 1B (Depdc1b) 10 ug
$289.00 $493.00
MR224877L3 Lenti ORF clone of Depdc1b (Myc-DDK-tagged) - Mouse DEP domain containing 1B (Depdc1b) 10 ug
$793.00
MR224877L4 Lenti ORF clone of Depdc1b (mGFP-tagged) - Mouse DEP domain containing 1B (Depdc1b) 10 ug
$793.00

Plasmid Map for MG224877

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG224877
Circular map for MG224877

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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