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Wisp3 (NM_001127376) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG223941
Wisp3 (tGFP-tagged) - Mouse WNT1 inducible signaling pathway protein 3 (Wisp3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wisp3
Synonyms CCN6; ENSMUSG00000062074; Gm735; WISP-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG223941 representing NM_001127376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTAGGCTCCTCTTTTGCACTCTGCTCATGACTGGGCTGACACAGCTCTGCTGCAGGACACAGGGCA
GTGCACCACAAGACTCGACCCCTGGAGGGCGGCCTGGAGCAGCATTGGAGGTGTATCAGCGTACAGAGGT
CTGCCGCTGGCCCTGCAGATGCCCCCCGCAGAGGCCCACTTGTCCTCCTGGGGTGAGTTTGGTGAGGGAC
GGCTGTGGATGCTGCAAAGTCTGTGCCAAGCAACCAGGGGACACCTGTAACGAAGCCGAGATCTGCGACC
CCCACAAAGGCCTGTACTGTGACTACTCCGGGGACACGCCTAGGTACGAGACAGGCGTGTGCGCATATCT
TGTGGCAGTTGGATGTGAGTTCAACAGGGTCTATTACCAGAATGGCCAAGTATTTCAGCCCCACCCACTG
TTCAGCTGCCTCTGTGTGAGTGGGGCCATTGGATGCACGCCCCTCTTCATCCCCAAGCTGGCTGGCAGCA
ACTGTTCTGCAGCTAAAGGAAGAAGGAAGACTGATCCCCCAAACTGTGGCCGGGGAACCCTGCAGCAGCA
GAACTCAGCAAGCTACAAGACAATGTCAGCTTATAGGAATCTCCCACTTACTTGGAGGAAAAAATGTCTC
GTGCAAGCAACCAAGTGGACTCCCTGCTCCAGAACGTGCGGAATGGGAATTTCTAACCGGGTAACCAACG
ATAACGCCAACTGTGAAATGAGAAAGGAGAGGAGGCTGTGCTACATCCAGCCCTGCAGCAGGAACACATC
ACAGGCAGTGAAGATCCCCAGAGGAGAAACATGTCAACCCACTTTCCAACTCCCCAAAGCTGAAAAATTT
GTTTTTTCTGGATGCTCAAGCACTCAGAGTTACAGACCCACTTTCTGTGGAATATGCCTGGACAAGAGAT
GCTGTGTCCCCAACAAATCTAAAATGATTACTGTTAGGTTTGACTGCCCCAGTGAAGGGTCATTTAAGTG
GCAGATGCTGTGGGTCACATCTTGTGTGTGTCAGAGGGACTGCAGAGAACCAGGAGATATATTTTCTGAG
CTCAGGATTCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG223941 representing NM_001127376
Red=Cloning site Green=Tags(s)

MRRLLFCTLLMTGLTQLCCRTQGSAPQDSTPGGRPGAALEVYQRTEVCRWPCRCPPQRPTCPPGVSLVRD
GCGCCKVCAKQPGDTCNEAEICDPHKGLYCDYSGDTPRYETGVCAYLVAVGCEFNRVYYQNGQVFQPHPL
FSCLCVSGAIGCTPLFIPKLAGSNCSAAKGRRKTDPPNCGRGTLQQQNSASYKTMSAYRNLPLTWRKKCL
VQATKWTPCSRTCGMGISNRVTNDNANCEMRKERRLCYIQPCSRNTSQAVKIPRGETCQPTFQLPKAEKF
VFSGCSSTQSYRPTFCGICLDKRCCVPNKSKMITVRFDCPSEGSFKWQMLWVTSCVCQRDCREPGDIFSE
LRIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001127376
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001127376.1, NP_001120848.1
RefSeq Size 1418 bp
RefSeq ORF 1065 bp
Locus ID 327743
UniProt ID D3Z5L9
Cytogenetics 10 B1
Summary Plays a role in mitochondrial electron transport and mitochondrial respiration.UniProtKB/Swiss-Prot Function
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"NM_001127376" in other vectors (4)
SKU Description Size Price
MC214501 Wisp3 (untagged) - Mouse WNT1 inducible signaling pathway protein 3 (Wisp3), (10ug) 10 ug
$457.00
MR223941 Wisp3 (Myc-DDK-tagged) - Mouse WNT1 inducible signaling pathway protein 3 (Wisp3) 10 ug
$289.00 $457.00
MR223941L3 Lenti ORF clone of Wisp3 (Myc-DDK-tagged) - Mouse WNT1 inducible signaling pathway protein 3 (Wisp3) 10 ug
$757.00
MR223941L4 Lenti ORF clone of Wisp3 (mGFP-tagged) - Mouse WNT1 inducible signaling pathway protein 3 (Wisp3) 10 ug
$757.00

Plasmid Map for MG223941

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG223941
Circular map for MG223941

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