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Arhgef7 (NM_017402) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG223397
Arhgef7 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7) transcript variant 3, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,102.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgef7
Synonyms betaPix; betaPix-b; betaPix-c; Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017402
ORF Size 1938 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017402.5
RefSeq Size 4488 bp
RefSeq ORF 1941 bp
Locus ID 54126
UniProt ID Q9ES28
Cytogenetics 8 A1.1
Summary Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).UniProtKB/Swiss-Prot Function
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"NM_017402" in other vectors (4)
SKU Description Size Price
MC220088 Arhgef7 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3, (10ug) 10 ug
$963.00
MR223397 Arhgef7 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 10 ug
$902.00
MR223397L3 Lenti ORF clone of Arhgef7 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 10 ug
$1,202.00
MR223397L4 Lenti ORF clone of Arhgef7 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 10 ug
$1,202.00

Plasmid Map for MG223397

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Cloning scheme for MG223397
Circular map for MG223397

file-alt Citations

Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/βPIX Proteins and Calcium
Sulimenko, Hájková, Černohorská et al
J. Immunol. (2015) 194 (9), 4099-111 DOI: 10.4049/jimmunol.1402459
Cited by 29
AI Summary: OriGene’s TurboGFP-tagged Git1 cDNA ORF clone (MG210556) was used to generate a GFP-fused mouse GIT1 construct, enabling the study of GIT1’s role in microtubule nucleation in mouse bone marrow-derived mast cells.
Snippet: A cassette encoding mouse GIT1 (mGIT1;Git1;RefSeq ID: NM_001004144.1) fused to GFP was amplified from the TurboGFP-tagged Git1 cDNA ORF clone (MG210556;OriGene Technologies) using forward 5′-GTCTAGAGAGGAGATCTGCCGCCG-3′ and reverse ... Cited by 1 Cite SaveSaving
Showing 1-1 of 1 papers.
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