Cacna1e (NM_009782) Mouse Tagged ORF Clone

SKU MG223012

Cacna1e (tGFP-tagged) - Mouse calcium channel voltage-dependent R type alpha 1E subunit (Cacna1e), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cacna1e
• Synonyms: A430040I15; alp; alpha1E; BII; Cach6; Cacnl1a6; Cav2; Cav2.3; Cchr; Cchra1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223012 representing NM_009782; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGGCCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA; GGAACCGACAAGGAACCCCCGTCCCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG; GGCGCGGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTG; TTCATCTTCGGAGAAGATAACATTGTCAGGAAATACGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGT; ACATGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGA; CAAGACCCCAATGTCTCGAAGACTGGAGAAGACAGAACCATATTTCATTGGGATCTTCTGCTTTGAAGCT; GGGATCAAAATTGTGGCTCTAGGGTTCATCTTCCATAAGGGTTCATACCTCCGTAATGGCTGGAATGTCA; TGGACTTCATCGTGGTCCTTAGTGGCATCCTGGCCACTGCGGGAACCCACTTCAACACCCACGTGGACCT; GCGGACCCTCCGGGCTGTGCGTGTCCTGAGGCCTTTAAAGCTGGTATCAGGAATACCTAGCCTGCAGATC; GTGCTGAAGTCTATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCC; TCATGTTTGCTATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTC; AGGTATTCTAGAGGGGTTTGATCCTCCTCACCCGTGTGGTGTGCAAGGCTGCCCGGCTGGTTATGAATGT; AAGGACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCCGTGCTGACTGTCT; TCCAATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTG; GAACTGGCTGTACTTCATCCCTCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTCGTCCTGGGAGTG; CTTTCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCGTTCATGAAGCTCCGGCGCC; AGCAACAGATTGAGCGAGAATTGAACGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGC; TGAAGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGG; ACGGAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTG; CCAGAGCCAGTATCAAGAGCACAAAAGTAGATGGGGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCG; CATCTCTATCCGCCACATGGTCAAGTCCCAAGTCTTTTACTGGATCGTCCTGAGTGTTGTGGCTCTTAAT; ACTGCCTGTGTGGCCATTGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAGT; TTTTGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTCTATTT; TCACTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTTGTCTGGGCAATC; TTCAGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAA; CCAAGTATTGGGCATCCCTCCGGAATCTGGTTGTCTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTT; ACTCTTCCTTCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTT; AACTTTAATGATGGGACTCCTTCAGCTAATTTTGACACCTTCCCTGCAGCTATCATGACAGTGTTCCAGA; TCCTGACAGGGGAAGACTGGAATGAGGTGATGTACAATGGCATTCGCTCCCAGGGTGGGGTCAGCTCAGG; CATGTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACGCTGCTAAATGTATTCTTG; GCCATCGCTGTGGACAATCTGGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCT; TCAACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCAT; TGAAAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGG; AGGCGCCGGCACCACATGTCTGTGTGGGAACAACGCACCAGCCAGCTGAGGAGGCACATGCAAATGTCTA; GCCAAGAAGCCCTCAACAAAGAGGAGGCCCCGCCAATGAACCCCCTCAATCCACTCAACCCACTGAGTCC; TCTCAACCCCCTCAATGCTCACCCCAGCCTTTATCGGAGGCCCAGGCCCATTGAGGGCCTAGCCCTAGGT; CTGGGTCTTGAAAAGTGTGAAGAAGAACGCATCAGTCGTGGGGGCTCCCTCAAGGGGGACATAGGGGGCC; TGACTAGCGCCCTGGACAACCAGAGGAGCCCCTTGTCCTTGGGCAAACGGGAGCCACCGTGGCTGCCTAG; ATCCTGTCATGGAAACTGTGACCCAATCCAGCAGGAGGCTGGGGGAGGAGAGACTGTGGTGACTTTTGAG; GACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAGGGCAAGGACT; CTGCCTCTGCATCCCGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGTCCGTTGAGGG; GGAGAAGGAACATGAGCCTCACAGCAGCCACAGGAGCAAAGAGCCGACCATCCACGAAGAGGAGAGAACC; CAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCTGGACTGGTAGGGGCTCTTGATGAAG; CAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGACAGAGCAGGA; GGCTGAAGGCAGCAGTGAACAAGCCCTTCTTGGGGACGTACAGCTGGATGTGGGCCGGGGCATTAGCCAG; AGTGAACCGGATCTCTCCTGCATGACAGCCAACATGGACAAGGCTACCACGGAGAGCACCAGTGTCACGG; TCGCCATCCCTGATGTGGACCCCTTGGTGGACTCGACAGTGGTGAACATTAGCAACAAGACGGATGGAGA; AGCCAGTCCCTTGAAGGAGGCAGAGACTAAAGAGGAAGAAGAAGAGGTAGAGAAGAAGAAGAAGCAGAAG; AAGGAGAAGCGAGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACCACTAACC; CGATCCGCAGGGCCTGCCACTACATTGTGAACCTACGCTACTTTGAAATGTGCATTCTCTTGGTGATTGC; GGCCAGCAGCATTGCCCTGGCAGCCGAGGACCCTGTACTAACTAACTCGGAGCGTAACAAAGTCCTGAGA; TATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGA; TCCTGCAGGATGGATCATACTTCAGAGACCTCTGGAACATCCTGGACTTTGTAGTAGTGGTTGGTGCATT; GGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGAGGGATATCAAGACTATCAAGTCTCTG; AGGGTACTTCGAGTTCTGCGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTGAAGGCTGTGTTTGACT; GCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTCATGTTCATCTTTGC; CGTCATCGCTGTTCAGCTATTCAAAGGAAAGTTCTTTTATTGCACTGACAGTTCCAAGGACACAGAGAAG; GAGTGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGTC; ATGAATTCCACTATGACAACATCATCTGGGCCCTGCTTACCCTCTTCACAGTCTCTACAGGGGAAGGATG; GCCTCAGGTTCTGCAGCACTCTGTAGATGTGACAGAGGAGGACAGAGGCCCAAGTCGCAGCAACCGCATG; GAGATGTCCATCTTTTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTCGTGGCTC; TCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTGGAGAAGAATGAGCG; GGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCACACCTTC; CAGTACCGTGTGTGGCACTTTGTAGTCTCCCCATCCTTTGAGTATACCATCATGGCCATGATTGCCTTGA; ACACTGTCGTGCTGATGATGAAGTACTACTCTGCTCCTTGCACCTATGAGCTCGCCTTAAAGTACCTGAA; CATCGCCTTCACTATGGTGTTTTCCCTGGAGTGTGTCCTAAAGGTCATTGCTTTCGGCTTCCTGAACTAT; TTCCGAGATACCTGGAACATCTTTGACTTCATCACGGTGATCGGCAGTATCACAGAAATTATCCTGACAG; ATAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCACGGCTCAT; AAAGCTTCTGAGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAAGGCTCTT; CCCTACGTCTGCCTTCTCATTGCCATGCTTTTCTTCATCTACGCCATCATTGGGATGCAGGTGTTTGGAA; ACATAAAGTTAGATGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGATCCCTCAT; GCTGCTTTTCAGGAGTGCCACGGGGGAGGCTTGGCAGGAGATTATGCTGTCCTGTCTCGGTGAGAAGGGT; TGTGAGCCTGACACCACTGCACCCTCAGGGCAGAATGAGAGTGAGCGCTGTGGCACTGACCTGGCCTACG; TTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTCGTGGCTGTCATCATGGA; CAACTTTGAGTACCTGACTCGAGATTCCTCCATCCTGGGGCCTCACCACTTAGATGAGTTTGTCCGTGTC; TGGGCAGAATATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCA; TGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGCTGGTCCTGATGAA; CATGCCAGTGGCTGAGGACATGACAGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTG; GACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCGGAGCTGCAAAAAGAGACCCTGG; CCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGAC; TGTGGGCAAGATCTATGCAGCAATGATGATCATGGACTACTATAAACAGAGTAAGGTAAAGAAACAGAGA; CAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCATCACTGCCTCAGGAGA; TCATTGCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTCGGAGTGG; ATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGCGGATGAT; GGACAATTCCAGGAACAACAATCTCTGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAA; TTCGGGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGCAGCAGTGAAAACACTTA; CAAGTCGCGGCGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCACACCGCCTGAATTCTGACTCTGGT; CACAAGTCTGACACTCACCGATCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCACCTCCTCT; CTCCAGATGTCTCCCGCTGCAATTCTGAAGAGCGAGGGACCCAGGCCGACTGGGAGTCCCCAGAACGTCG; CCAGTCCAGGTCACCCAGTGAAGGAAGGTCACAGACCCCCAATAGACAGGGTACCGGTTCCCTAAGTGAG; AGCTCCATCCCCTCTATTTCCGACACCAGCACCCCAAGACGAAGCCGTCGCCAGCTCCCACCTGTGCCAC; CAAAGCCTCGTCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGA; TGGAAGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAGAGCAACAGCGCTTGCCTGACCGAGTCT; TCCAACTCCTTGCACCCCCAGCAGGGCCAGCATCCTTCCCCACAGCACTACATCTCCGAGCCCTATCTGG; CCCTCCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCCGTAGC; CACTAGCTTGGGCCGGTCCAATACCATTGGCTCTGCGCCACCCCTACGGCACAGCTGGCAGATGCCTAAT; GGGCACTATCGGCGGCGGAGGCGTGGGGGGCCTGGGCCTGGCATGATGTGTGGAGCTGTCAGTGACCTCC; TGAGTGACACGGAAGAAGACGATAAGTGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223012 representing NM_009782; Red=Cloning site Green=Tags(s); ; MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSL; FIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEA; GIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQI; VLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYEC; KDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGV; LSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSR; TEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALN; TACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAI; FRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRF; NFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFL; AIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRER; RRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALG; LGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFE; DRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERT; QDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQ; SEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQK; KEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLR; YFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSL; RVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEK; ECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRM; EMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTF; QYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNY; FRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKAL; PYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKG; CEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRV; WAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTAL; DIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQR; QQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADD; GQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSG; HKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSE; SSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTES; SNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPN; GHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009782
• ORF Size: 6819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009782.3, NP_033912.2
• RefSeq Size: 12697 bp
• RefSeq ORF: 6822 bp
• Locus ID: 12290
• Cytogenetics: 1 66.14 cM
• Summary: This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]