Rax (NM_013833) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG222800
Rax (tGFP-tagged) - Mouse retina and anterior neural fold homeobox (Rax), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rax
Synonyms 7530406A22Rik; E130303K03Rik; ey-1; ey1; Rx
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222800 representing NM_013833
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCTGCCGGGCTGCGCGCCAGCCATGGCGGACGGGAGCTTCTCGCTCGCTGGCCACCTGCTCCGCA
GCCCGGGCGGGAGCACGTCGCGTTTGCACAGCATCGAAGCCATCCTGGGCTTTACCAAGGAAGACGGCAT
CCTAGACACCTTTCCTGCAGAGAGGAGCTCCAGGAGCTCGAAAGAGCGGGATCCCAGGCTGGGCGCGCAG
CCTGCCTGCCCCAAGGCGCCAGCCGGAGGCTCCGAGTCTTCCCCACCAGCAGCCCCGGGATTCGTCCCGG
AGTACGAAGCTACTAGGCCCTGCTACCCCAAGGAGCAAGGAGAAGCACGTCCGAGCCCAGGACTCTCGGT
TGGGCCAGCAGCAGGCGACTCGAAGCTGTCGGAGGAGGAACCTCCCAAGAAGAAGCATCGACGCAACCGC
ACGACGTTCACCACTTACCAACTGCACGAGCTGGAGCGCGCCTTCGAGAAGTCCCACTACCCCGACGTGT
ACAGCCGCGAAGAGCTGGCCGGCAAGGTTAACCTACCCGAGGTTCGGGTCCAGGTATGGTTCCAGAACCG
ACGGGCTAAGTGGAGGCGCCAGGAGAAACTGGAAGTGTCGTCCATGAAGCTGCAGGATTCTCCCCTCCTC
TCCTTCAGCCGCTCCCCGCCTTCCTCGGCACTGGCCCCGCTGGGAACCGGCCCGGGCAGCGGGAGCGGAC
CTCCGGGGAGCGCCCTGCCGCTGGAGCCGTGGCTTGGGCCACCGCTGCCCGGAGGAGGCGCCACAGCGCT
GCAGAGCCTGCCGGGATTCGGGCCGCCGGGGCAGGGTCTCCCGGCCAGCTACACGCCACCACCGCCCTTC
CTGAACTCCGCGCCCCTGGGTCCAGGTCTGCAGCAGCTCGGGCCACCGCCCGCGTACCCCTGCGCGCCGG
CATTCGGGGACAAGTTCTCGCTGGAAGAGGCGTACCCGCGCAACAGCAGCATCGCCGCTCTGCGCCTGAA
GGCCAAAGAGCACATCCAGGCCATCGGAAAGCCCTGGCAAGCCCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222800 representing NM_013833
Red=Cloning site Green=Tags(s)

MHLPGCAPAMADGSFSLAGHLLRSPGGSTSRLHSIEAILGFTKEDGILDTFPAERSSRSSKERDPRLGAQ
PACPKAPAGGSESSPPAAPGFVPEYEATRPCYPKEQGEARPSPGLSVGPAAGDSKLSEEEPPKKKHRRNR
TTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSMKLQDSPLL
SFSRSPPSSALAPLGTGPGSGSGPPGSALPLEPWLGPPLPGGGATALQSLPGFGPPGQGLPASYTPPPPF
LNSAPLGPGLQQLGPPPAYPCAPAFGDKFSLEEAYPRNSSIAALRLKAKEHIQAIGKPWQAL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013833
ORF Size 1026 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013833.2, NP_038861.2
RefSeq Size 1687 bp
RefSeq ORF 1029 bp
Locus ID 19434
UniProt ID O35602
Cytogenetics 18 39.03 cM
Summary Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. Binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013833" in other vectors (4)
SKU Description Size Price
MC209296 Rax (untagged) - Mouse retina and anterior neural fold homeobox (Rax), (10ug) 10 ug
$503.00
MR222800 Rax (Myc-DDK-tagged) - Mouse retina and anterior neural fold homeobox (Rax) 10 ug
$289.00 $457.00
MR222800L3 Lenti ORF clone of Rax (Myc-DDK-tagged) - Mouse retina and anterior neural fold homeobox (Rax) 10 ug
$757.00
MR222800L4 Lenti ORF clone of Rax (mGFP-tagged) - Mouse retina and anterior neural fold homeobox (Rax) 10 ug
$757.00

Plasmid Map for MG222800

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Cloning scheme for MG222800

Circular map for MG222800

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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