Tex19.2 (NM_027622) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG222451
Tex19.2 (tGFP-tagged) - Mouse testis expressed gene 19.2 (Tex19.2), (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tex19.2
Synonyms 4921530G04Rik; Tex19b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222451 representing NM_027622
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTCCCCCAGTCAGCGTTCGCCATGGGGCCAAGGGCATGTCCTGCCTCTATGGGGCGTGGCTGTACC
ATCTTGTCCATGGGGAACAGATGAAGATCTGCTTTGCTTGCTTCAAGGCAGCTTTCCTCGTCGTTAAGAA
CATGCTGGAGATGGGAGACTGGGAAGAAGGAGTGTGGGATGCTGAGCCCATGGAACTCTCAGAGGCATCG
TCTGAGCCCGAGGAATGGCCCGGGCTTAGTGGGGGAGAGGGCCAAGGTCATCTGCCACATGGCATCTCTG
TCTCTGCAGGCTCTGGGGCTCAGGGACCCCAGCCTGTGCCCACTGAGCTGGGGCCTCAGGAAGCTGTACC
CCTGGATCTGGGTCCTGAGGATGCTGAGTGGACCCAGGCCCTTCCCTGGAGATTTGATGGGCTTTCACCC
TGCTCCCACTGGCTCATCCCTCCTTTGTCCTGGTGGGAGATTTTCAATGTAAGCCCATCTCCTGGGCAAC
CTGTATTGTTGGAGTTGAGCCCCACCTGGCCCATGGACCCGTTGGAAGCAGAAGCATGGTTGGTAGGCCT
GAAGTTCGTTTTCCTTCTGGGTGGCTTTGACGCCATTTGCTACATGCTGTCAATGACTCCCTGTTGGGCC
GTGAGAACCCGTGTCCAGCGCTGGCAGGTGTTGCTGGACCCTGGTGACGTGAGAGTGGCCCAGCTGCAGA
ATGCACCTGAACAGCAAGACCTGCACCGCTGGAAGCTAAGCGTCCTGGAGTCCTCAGAGCTGGGGATGGA
GCTGGTGCCTGCTGACTGCAGCCTACAAAAGGGAGGCTTCAAGGTGCATTCGTATTTGCCCTGGCACAAT
AGCACCCCAGAGTCCTGGAGCAGGGAGCCAGGGGAGAGGCTCCTTGTGGTAGAAGTTGTATCTCTGAGGG
AGTTGCCCTGCTTCCGTTCCCCCTCCCCTGATCCACACAAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222451 representing NM_027622
Red=Cloning site Green=Tags(s)

MCPPVSVRHGAKGMSCLYGAWLYHLVHGEQMKICFACFKAAFLVVKNMLEMGDWEEGVWDAEPMELSEAS
SEPEEWPGLSGGEGQGHLPHGISVSAGSGAQGPQPVPTELGPQEAVPLDLGPEDAEWTQALPWRFDGLSP
CSHWLIPPLSWWEIFNVSPSPGQPVLLELSPTWPMDPLEAEAWLVGLKFVFLLGGFDAICYMLSMTPCWA
VRTRVQRWQVLLDPGDVRVAQLQNAPEQQDLHRWKLSVLESSELGMELVPADCSLQKGGFKVHSYLPWHN
STPESWSREPGERLLVVEVVSLRELPCFRSPSPDPHN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027622
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027622.3, NP_081898.1
RefSeq Size 2046 bp
RefSeq ORF 954 bp
Locus ID 70956
UniProt ID Q9D5S1
Cytogenetics 11 E2
Summary May be required during spermatogenesis, probably by participating in the repression of retrotransposable elements and prevent their mobilization (Probable). With its paralog, Tex19.1, collaborates with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Interacts with Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:28254886).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027622" in other vectors (4)
SKU Description Size Price
MC211119 Tex19.2 (untagged) - Mouse testis expressed gene 19.2 (Tex19.2), (10ug) 10 ug
$330.00
MR222451 Tex19.2 (Myc-DDK-tagged) - Mouse testis expressed gene 19.2 (Tex19.2) 10 ug
$289.00 $300.00
MR222451L3 Lenti ORF clone of Tex19.2 (Myc-DDK-tagged) - Mouse testis expressed gene 19.2 (Tex19.2) 10 ug
$600.00
MR222451L4 Lenti ORF clone of Tex19.2 (mGFP-tagged) - Mouse testis expressed gene 19.2 (Tex19.2) 10 ug
$600.00

Plasmid Map for MG222451

Download sequence for

Cloning scheme for MG222451

Circular map for MG222451

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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