Dusp5 (NM_001085390) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG221293
Dusp5 (tGFP-tagged) - Mouse dual specificity phosphatase 5 (Dusp5), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp5
Synonyms Gm337
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG221293 representing NM_001085390
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTCACGTCGCTCGACGGGCGCCAGCTGCGCAAGATGCTCCGCAAGGAGGCGGAGGCGCGCTGCG
TGGTGCTCGACTGCCGGCCCTACCTGGCCTTCGCCGCGTCGAGCGTGCGCGGCTCGCTCAACGTCAACCT
CAACTCGGTGGTGCTGCGGCGGGCCCGGGGCGGCGCGGTGTCGGCGCGCTACGTGCTGCCCGACGAGGCG
GCCCGCGCTCGGCTGCTGCAGGAGGGCGGCGGCGGTGTGGCGGCGGTGGTCGTGCTGGACCAGGGTAGCC
GCCACTGGCAGAAGCTGCGGGAGGAGAGCGCCGCGCGCGTCGTGCTCACCTCGCTGCTGGCCTGCCTGCC
CGCCGGCCCGCGGGTCTACTTCCTTAAAGGGGGGTATGAGACCTTCTACTCACAGTATCCTGAGTGCTGT
GTGGATGTGAAGCCCACCTCACAAGAGAAGATCGAAGGCGAGAGAAGCCTCCTCAGCCAGTGTGGAAAGC
CCGTTCTCAGCGTCGCCTACAGACCAGCCTATGACCAGGGTGGCCCGGTTGAAATCCTTCCCTTCCTCTA
CCTTGGAAGTGCCTACCACGCATCCAAGTGCGAGTTCCTCGCCAACCTGCACATCACAGCCCTGCTGAAT
GTTTCCCGCCGGACCTCCGAGGCCTGCACCACCCACCTACACTACAAGTGGATCCCCGTGGAGGACAGCC
ACACTGCTGACATTAGCTCCCACTTTCAAGAAGCAATAGATTTTATTGACTGTGTCAGGGAAGAAGGAGG
CAAGGTCCTGGTCCACTGCGAAGCTGGGGTTTCCCGGTCGCCCACCATCTGCATGGCTTACCTCATGAAG
ACCAAGCAGTTCCGGCTGAAGGAGGCCTTCGATTACGTCAAGCAGAGGAGGAGCGTGGTCTCTCCCAACT
TTGGCTTCATGGGACAGCTCCTTCAGTATGAATCTGAGATCCTGCCCTCCACACCCACCCTCCAGCCGCC
CTCCTGCCAAGGGGAGGCAGCCAGCTCCACGTTTATAGGCCATTTACAGACACTGAGCCCTGATATGCAG
GGCACCTACTGCACATTCCGTACCTCAGTGCTGGCACCGGTGCCCACCCACTCCACAGTCCCAGAGCTCC
ACAGGAGCCCCGTGGCCACAGCCACATCCTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG221293 representing NM_001085390
Red=Cloning site Green=Tags(s)

MKVTSLDGRQLRKMLRKEAEARCVVLDCRPYLAFAASSVRGSLNVNLNSVVLRRARGGAVSARYVLPDEA
ARARLLQEGGGGVAAVVVLDQGSRHWQKLREESAARVVLTSLLACLPAGPRVYFLKGGYETFYSQYPECC
VDVKPTSQEKIEGERSLLSQCGKPVLSVAYRPAYDQGGPVEILPFLYLGSAYHASKCEFLANLHITALLN
VSRRTSEACTTHLHYKWIPVEDSHTADISSHFQEAIDFIDCVREEGGKVLVHCEAGVSRSPTICMAYLMK
TKQFRLKEAFDYVKQRRSVVSPNFGFMGQLLQYESEILPSTPTLQPPSCQGEAASSTFIGHLQTLSPDMQ
GTYCTFRTSVLAPVPTHSTVPELHRSPVATATSC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001085390
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001085390.2
RefSeq Size 1155 bp
RefSeq ORF 1155 bp
Locus ID 240672
Cytogenetics 19 D2
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Documents
Resources
"NM_001085390" in other vectors (4)
SKU Description Size Price
MC213106 Dusp5 (untagged) - Mouse dual specificity phosphatase 5 (Dusp5), (10ug) 10 ug
$457.00
MR221293 Dusp5 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 5 (Dusp5) 10 ug
$289.00 $457.00
MR221293L3 Lenti ORF clone of Dusp5 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 5 (Dusp5) 10 ug
$757.00
MR221293L4 Lenti ORF clone of Dusp5 (mGFP-tagged) - Mouse dual specificity phosphatase 5 (Dusp5) 10 ug
$757.00

Plasmid Map for MG221293

Download sequence for

Cloning scheme for MG221293

Circular map for MG221293

file-alt Citations

Endoplasmic Reticulum Stress Increases DUSP5 Expression via PERK-CHOP Pathway, Leading to Hepatocyte Death
Jo, Yang, Park et al
Int J Mol Sci (2019) 20 (18) DOI: 10.3390/ijms20184369
AI Summary: OriGene products, specifically the Myc-flag-tagged CHOP (NM_007837) and Myc-flag-tagged DUSP5 (NM_001085390) ORF clones, were used to study the roles of CHOP and DUSP5 in ER stress-induced hepatocyte death by enabling their overexpression in cellular experiments.
Snippet: manufacturer's instructions. Myc-flag-tagged CHOP (NM_007837) and Myc-flag-tagged DUSP5 (NM_001085390) open reading frame (ORF) clones were obtained from OriGene Technologies (Rockville, MD, USA). Scrambled Cached
Showing 1-1 of 1 papers.
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