Cdc26 (NM_139291) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG220401
Cdc26 (tGFP-tagged) - Mouse cell division cycle 26 (Cdc26), (10ug)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdc26
Synonyms 2010012C09Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG220401 representing NM_139291
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGACGGAAACCGACCCGCTTAGAGCTCAAGCTCGACGACATTGAGGAGTTTGAGAGCATTCGCA
AGGACCTGGAGGCCCGTAAGAAGCAGAAGGAAGATGTGGAAGGTGTAGGAACCAGCGATGGAGAAGGAGC
TGCTGGGCTCAGCAGTGACCCCAAAAGCCGGGAACAAATGATTAATGATCGAATTGGTTATAAACCCCAA
CTCAAGAGCAACAACCGCACATCTCAGTTTGGAAACTTTGAATTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG220401 representing NM_139291
Red=Cloning site Green=Tags(s)

MLRRKPTRLELKLDDIEEFESIRKDLEARKKQKEDVEGVGTSDGEGAAGLSSDPKSREQMINDRIGYKPQ
LKSNNRTSQFGNFEF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139291
ORF Size 255 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139291.4
RefSeq Size 794 bp
RefSeq ORF 258 bp
Locus ID 66440
UniProt ID Q99JP4
Cytogenetics 4 B3
Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139291" in other vectors (4)
SKU Description Size Price
MC210368 Cdc26 (untagged) - Mouse cell division cycle 26 (Cdc26), (10ug) 10 ug
$165.00
MR220401 Cdc26 (Myc-DDK-tagged) - Mouse cell division cycle 26 (Cdc26) 10 ug
$289.00
MR220401L3 Lenti ORF clone of Cdc26 (Myc-DDK-tagged) - Mouse cell division cycle 26 (Cdc26) 10 ug
$450.00
MR220401L4 Lenti ORF clone of Cdc26 (mGFP-tagged) - Mouse cell division cycle 26 (Cdc26) 10 ug
$450.00

Plasmid Map for MG220401

Download sequence for

Cloning scheme for MG220401

Circular map for MG220401

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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