Itih1 (NM_008406) Mouse Tagged ORF Clone

SKU MG220169

Itih1 (tGFP-tagged) - Mouse inter-alpha trypsin inhibitor heavy chain 1 (Itih1), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Itih1
• Synonyms: In; Intin1; ITI-HC1; Itih; Itih-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220169 representing NM_008406; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGGTGCCACAGGGCTCCGGGTGCTGCTGTGCTTGCTGCCGCTCCTCACTCTGCAGGCCAGGCCTG; CTTTGGGCTTGGCAACAGGCAGACCCAGGGGCAGTGAGAAGCGACACGCTGTGGACACTTCAGTCAATGG; TGTGTCCATCAAGAGCTTGAAAGTCAATTGCAAAGTCACCTCTCGCTTTGCCCACTACGTCATCACCAGC; CAAGTGGTCAACAATGCTGATAAAGCCAGGGAAGTGGCTTTTGATGTGGAAATCCCCAAGACAGCCTTCA; TCAGTGACTTTGCCATAACATCAGATGGGAAGGCGTTCATTGGGGACATAAAGGACAAAGTGACTGCATG; GAAGCAGTACCGGAAGGCAGCCGTTTTAGGGGAGAGCGCTGGCCTTGTCAGAGCCTCGGGCAGAAATATG; GAGCAGTTCACCATCCATATCACTGTTGGAGCCCAGAGCAAGGCCACGTTCCGGCTCACCTATGAGGAGG; TACTAAAGAGGAGACTGATGCAGTATGACATCACCATCAAAGTCAGACCCAAGCAGCTGGTACAGCATTT; TGAGATCGATGTGGACATCTTTGAACCCCAGGGGATCAGCAAGCTGGACGCTCAGGCCTCCTTCCTCAGT; GAGGAACTGGCTGCTCAAACCATCAAGAAGTCTTTCTCAGGGAAAAAGGGTCATGTGCTCTTCCGCCCCA; CTGTGAGCCAGCAGCAGTCCTGCCCCACATGTTCTACATCCTTGCTAAATGGAGAATTCAAGGTGACCTA; CGATGTCAATCGAGACAAGCTCTGTGACCTCCTGGTGGCCAACAACTACTTTACACATTTCTTTGCCCCC; AAAAACCTGACCAACATGAGCAAGAACCTGGTTTTTGTGATTGACATCAGTGGCTCCATGGAAGGCCAGA; AAGTGAGGCAGACAAAGGAGGCACTCCTTAAGATCCTGGAGGACATGAGGCCAGTAGACAACTTTGACCT; GGTCCTCTTTGGCTCTAAAGTGCAATCATGGAAGGGCTCCCTGGTACCTGCGTCTAATGCCAATCTGCAA; GCAGCTCAAGACTTTGTGAGGCGCTTTTCTTTGGCTGGAGCCACAAACCTGAATGGAGGCTTGCTCCGAG; GAATCGAGATCTTAAACAAAGCTCAAGGAAGCCACCCAGAACTCAGCAGCCCGGCCTCAATTCTTATCAT; GTTGACAGACGGAGAGCCCACTGAAGGGGAGACGGACCGTTCCCAGATCCTCAAGAATGTACGCAACGCT; ATCCGGGGCAGGTTCCCGCTCTACAACCTCGGCTTTGGCCACGACCTGGACTTCAGCTTCCTGGAGGTCA; TGTCCACTGAGAACAACGGATGGGCCCAGAGAATTTATGAGGACCATGATGCCACCCAACAGCTACAGGG; TTTCTACAATCAAGTAGCCAATCCCCTGCTGACAGATGTGGAACTGCAGTACCCCCAGGATGCTGTCTTG; GCTCTAACCCAGCACCGACATAAACAGTACTATGACGGCTCGGAGATTGTGGTGGCTGGGCGCATTGCTA; ACCACAAACTGAACACCTTTAAGGCTGACGTTCGGGCTCGTGGGGAAAAGCAAGAATTCAGGGCAACCTG; CCTAGTGGACGAGGAAGAGATGAAGAAACTGCTCCGAGAGCGTGGCCACGTGCTAGAGAATCATGTGGAG; AGGCTCTGGGCCTACCTCACGATCCAGGAGCTCCTGGCTAAGCGGATGAAGACGGAAGGGGAGGAGAGGG; CCAACCTGTCATCCCAGGTCCTGAAGATGTCACTGGACTATCATTTCGTAACGCCACTGACCTCTCTGAC; TATCAGAGGCCTGACAGACGAGGATGGGCTGGAGCCCACCATTGACAAGCCCCTGGAGGATTCTCAGCCC; TTAGAGATGGTGGGACCCAGAAGAACGTTTGTGCTGTCAGCCATACAGCCATCTCCTACAGCCCACCCCA; TAGACTCAAAGTTGCCACTCCGTGTGACAGGTGTGGACACCGATCCCCACTTCATCATCTATGTGCCCTC; AAAAGAGGATAGCTTGTGCTTCAACATCAATGAGGAACCTGGTGTGATCCTGAACCTAGTACAGGACCCT; GACACAGGCTTCACCGTGAATGGGCAGCTCATTGGGAACAAGGCCAGCAGCCCTGGGCAACACGAGAGCA; CATACTTCGGGAGATTGGGAATCTCAAGTCCCACATCAGACTTTCAGCTGGAAGTGACTCCTCAGAACAT; TACACTGAACCCCAGCTCCAGCGGGTCCATGTTCTCCTGGAGGGACCAGGCTGTGCTGCAGAAGGATGGG; GTAGTGGTGACAATCAACAAGAAGAGGAACCTGGTGGTGTCTGTGGATGATGGGGCTACCTTTGAGATTG; TCCTGCACAGAACATGGAAGGGGAGTGCAGTCCACCAGGACTTCCTGGGTTTCTATGTGCTGGACAGCTT; CCGGATGTCAGCCCGGACAAAGGGGCTACTGGGACAATTCTTCTCCCCACTGGATTTTGAAGTATTTGAC; CTCCACCCAGGCTCTGACCCTACAAAGACAGATGCCACAATGGTGGTGAAGAATCGGCAGCTGACAGTCA; CCCGAGGGTTGCAAAAAGACTACAGCAAGGACCCCAGACATGGAGCAGAAGTGCCTTGCTGGTTTGTCCA; CGACAATGGAGCTGGACTGATTGATGGTGTTCACACTGACTATGTCGTCTCTGACATCTTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220169 representing NM_008406; Red=Cloning site Green=Tags(s); ; MEGATGLRVLLCLLPLLTLQARPALGLATGRPRGSEKRHAVDTSVNGVSIKSLKVNCKVTSRFAHYVITS; QVVNNADKAREVAFDVEIPKTAFISDFAITSDGKAFIGDIKDKVTAWKQYRKAAVLGESAGLVRASGRNM; EQFTIHITVGAQSKATFRLTYEEVLKRRLMQYDITIKVRPKQLVQHFEIDVDIFEPQGISKLDAQASFLS; EELAAQTIKKSFSGKKGHVLFRPTVSQQQSCPTCSTSLLNGEFKVTYDVNRDKLCDLLVANNYFTHFFAP; KNLTNMSKNLVFVIDISGSMEGQKVRQTKEALLKILEDMRPVDNFDLVLFGSKVQSWKGSLVPASNANLQ; AAQDFVRRFSLAGATNLNGGLLRGIEILNKAQGSHPELSSPASILIMLTDGEPTEGETDRSQILKNVRNA; IRGRFPLYNLGFGHDLDFSFLEVMSTENNGWAQRIYEDHDATQQLQGFYNQVANPLLTDVELQYPQDAVL; ALTQHRHKQYYDGSEIVVAGRIANHKLNTFKADVRARGEKQEFRATCLVDEEEMKKLLRERGHVLENHVE; RLWAYLTIQELLAKRMKTEGEERANLSSQVLKMSLDYHFVTPLTSLTIRGLTDEDGLEPTIDKPLEDSQP; LEMVGPRRTFVLSAIQPSPTAHPIDSKLPLRVTGVDTDPHFIIYVPSKEDSLCFNINEEPGVILNLVQDP; DTGFTVNGQLIGNKASSPGQHESTYFGRLGISSPTSDFQLEVTPQNITLNPSSSGSMFSWRDQAVLQKDG; VVVTINKKRNLVVSVDDGATFEIVLHRTWKGSAVHQDFLGFYVLDSFRMSARTKGLLGQFFSPLDFEVFD; LHPGSDPTKTDATMVVKNRQLTVTRGLQKDYSKDPRHGAEVPCWFVHDNGAGLIDGVHTDYVVSDIF; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008406
• ORF Size: 2721 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008406.3, NP_032432.2
• RefSeq Size: 3077 bp
• RefSeq ORF: 2724 bp
• Locus ID: 16424
• UniProt ID: Q61702
• Cytogenetics: 14 B
• Summary: This gene encodes a heavy chain of inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors. IaI proteins are protein-glycosaminoglycan-protein complexes comprised of two heavy chains and a light chain. The encoded protein covalently associates with the light chain via a chondroitin sulfate moiety. Intravenous administration of the encoded protein improved survival of mice after infection with Escherichia coli. This gene is located adjacent to two other IaI heavy chain genes. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature protein. [provided by RefSeq, Oct 2015]