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BC147527 (NM_001037925) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG219475
BC147527 (tGFP-tagged) - Mouse 2-cell-stage variable group member 3-like (LOC625360), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC147527
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219475 representing NM_001037925
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAAGCCAAGAAGATGATGCTGTCCTTCCCCGGTTTTGTCAAGGATACATCAGATACTGAAGAAC
ATGCACTGCCCAGTGCACATGCCTTGGCAGCCCAGAGTACAAGGTGCTCCAAGTCTGAGACATTTTGTTT
AAGCAAAGAGCAAAGCCACTGCTCTGAGGATGGCTGGGTTTTAGAATGGGAAATTTGTGTGTTTGAGAGT
GTGGACTACCTGGATTCCTACCACAAATTTAATTGTACCATGAAGAAGAGCACAGAAGTCTTCCAGAGTG
AGTGTCAGAGGGAGTCACAAGTGTCCCCAGGAGATGTGGAAAACAACAAAGACAAAGATACAGAGGAGCC
AGACCAACCCTCACCAAGCTTGCTCAGGGAGAAAGGGCTGGAAGTTGAGACCTGTGATGGTGGAGACTGC
CCTGACAAGGATCCTGCATCTTACAGTGCCAGGCACCTAGGCTGCTGGGCATGGCTTCAAAGAGCTTTTC
GCCAGAAGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219475 representing NM_001037925
Red=Cloning site Green=Tags(s)

MEKAKKMMLSFPGFVKDTSDTEEHALPSAHALAAQSTRCSKSETFCLSKEQSHCSEDGWVLEWEICVFES
VDYLDSYHKFNCTMKKSTEVFQSECQRESQVSPGDVENNKDKDTEEPDQPSPSLLREKGLEVETCDGGDC
PDKDPASYSARHLGCWAWLQRAFRQKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001037925
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001037925.2, NP_001033014.1
RefSeq Size 1678 bp
RefSeq ORF 504 bp
Locus ID 625360
Cytogenetics 13
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"NM_001037925" in other vectors (4)
SKU Description Size Price
MC215243 BC147527 (untagged) - Mouse cDNA sequence BC147527 (BC147527), (10ug) 10 ug
$330.00
MR219475 BC147527 (Myc-DDK-tagged) - Mouse cDNA sequence BC147527 (BC147527) 10 ug
$289.00 $300.00
MR219475L3 Lenti ORF clone of BC147527 (Myc-DDK-tagged) - Mouse cDNA sequence BC147527 (BC147527) 10 ug
$600.00
MR219475L4 Lenti ORF clone of BC147527 (mGFP-tagged) - Mouse cDNA sequence BC147527 (BC147527) 10 ug
$600.00

Plasmid Map for MG219475

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG219475
Circular map for MG219475

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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