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Atic (NM_026195) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG219425
Atic (tGFP-tagged) - Mouse 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (Atic), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $751.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atic
Synonyms 2610509C24Rik; AA536954; AW212393
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219425 representing NM_026195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCGTCTCAGCTTGCCCTGTTCAGTGTGTCTGACAAAACTGGCCTCGTGGAATTTGCCAGAAGTC
TCGCGTCTCTTGGTTTGAGTCTGGTCGCTTCCGGAGGCACGGCAAAAGCGATCAGGGATGCTGGCCTGGC
AGTCAGAGATGTGTCTGAGCTAACAGGGTTCCCTGAAATGTTAGGGGGACGCGTGAAAACCTTGCACCCT
GCGGTCCATGCTGGGATCTTGGCACGCAACATTCCAGAAGACGCTGCAGACATGGCCAGGCTTGATTTCA
ACCTTGTAAGGGTTGTGGTCTGTAACCTGTACCCCTTCGTGAAGACTGTGGCTTCTCCAGATGTAACTGT
TGAGGCAGCTGTTGAACAAATTGATATTGGTGGCGTGACATTACTGAGAGCTGCAGCCAAAAACCACGCT
CGAGTGACAGTCGTCTGTGAACCAGAGGACTATGCGGGGGTAGCTGCAGAGATGCACGGCTCTGATAGTA
AGGACACCTCCCTAGAGACCAGGCGCCACTTGGCCTTGAAGGCATTCACTCATACCGCTCAATACGATGA
AGCAATTTCAGATTACTTCAGGAAACAGTACAGTAAGGGAATTTCTCAGATGCCCTTGCGGTATGGGATG
AACCCCCATCAGACTCCTGCCCAGTCGTATACGCTGAAGCCCAAACTTCCCATCACAGTTCTCAATGGAG
CCCCAGGATTCATAAACTTGTGTGACGCTTTGAACGCCTGGCAGCTGGTGACAGAGCTGAGAGGAGCAGT
AGACATCCCAGCGGCTGCTTCTTTCAAGCACGTCAGCCCGGCAGGTGCTGCTGTTGGGGTTCCACTCAGT
GAGGACGAGGCCAGAGTCTGCATGGTCTATGACCTCTACCCGACCCTCACGCCCCTGGCCGTTGCATATG
CAAGAGCAAGAGGAGCTGATAGGATGTCTTCATTTGGTGATTTTGTTGCATTGTCTGACATTTGTGATGT
CCCAACTGCAAAAATTATCTCCAGAGAAGTGTCGGATGGTATTGTTGCCCCGGGATATGAAGAAGAAGCC
TTGAAAATACTTTCTAAAAAGAAAAATGGAAACTACTGTGTTCTTCAGATGGACCAGTCCTACAAACCAG
ATGAAAATGAAGTTCGAACACTCTTTGGTCTTCGTTTAAGCCAGAAGAGAAATAATGGTGTCGTTGACAA
GTCATTATTTAGCAATATTGTCACCAAGAATAAAGATCTGCCGGAGTCTGCACTCAGAGACCTCATTGTC
GCCACCGTCGCTGTCAAGTATACTCAGTCTAATTCGGTGTGCTATGCCAAGGATGGGCAGGTTATTGGCA
TCGGAGCAGGACAGCAGTCTCGGATACACTGCACTCGCCTTGCAGGTGATAAGGCAAACTCTTGGTGGCT
TCGGCACCACCCACGAGTGCTGTCCATGAAGTTTAAAGCCGGGGTGAAGAGAGCAGAGATCTCCAATGCG
ATTGATCAGTATGTGACCGGCACTATCGGTGAGGGCGAAGACCTGGTGAAATGGGAAGCACTGTTCGAAG
AGGTTCCTGAGTTACTCACGGAGGCGGAGAAGAAGGAATGGGTGGACAAGCTGAGCGGCGTGTCTGTCAG
CTCCGACGCCTTCTTTCCTTTCAGGGATAATGTGGACCGAGCTAAAAGGAGTGGTGTGGCCTACATTGTC
GCTCCATCTGGATCTACTGCTGACAAGGTTGTGATTGAGGCGTGCGACGAGCTGGGGATTGTCTTGGCTC
ATACGGATCTCCGGCTCTTCCACCAC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219425 representing NM_026195
Red=Cloning site Green=Tags(s)

MAPSQLALFSVSDKTGLVEFARSLASLGLSLVASGGTAKAIRDAGLAVRDVSELTGFPEMLGGRVKTLHP
AVHAGILARNIPEDAADMARLDFNLVRVVVCNLYPFVKTVASPDVTVEAAVEQIDIGGVTLLRAAAKNHA
RVTVVCEPEDYAGVAAEMHGSDSKDTSLETRRHLALKAFTHTAQYDEAISDYFRKQYSKGISQMPLRYGM
NPHQTPAQSYTLKPKLPITVLNGAPGFINLCDALNAWQLVTELRGAVDIPAAASFKHVSPAGAAVGVPLS
EDEARVCMVYDLYPTLTPLAVAYARARGADRMSSFGDFVALSDICDVPTAKIISREVSDGIVAPGYEEEA
LKILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLRLSQKRNNGVVDKSLFSNIVTKNKDLPESALRDLIV
ATVAVKYTQSNSVCYAKDGQVIGIGAGQQSRIHCTRLAGDKANSWWLRHHPRVLSMKFKAGVKRAEISNA
IDQYVTGTIGEGEDLVKWEALFEEVPELLTEAEKKEWVDKLSGVSVSSDAFFPFRDNVDRAKRSGVAYIV
APSGSTADKVVIEACDELGIVLAHTDLRLFHH

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_026195
ORF Size 1776 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026195.3, NP_080471.2
RefSeq Size 2611 bp
RefSeq ORF 1779 bp
Locus ID 108147
UniProt ID Q9CWJ9
Cytogenetics 1 C3
Summary Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026195" in other vectors (4)
SKU Description Size Price
MC219447 Atic (untagged) - Mouse 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (Atic), (10ug) 10 ug
$607.00
MR219425 Atic (Myc-DDK-tagged) - Mouse 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (Atic) 10 ug
$289.00 $551.00
MR219425L3 Lenti ORF clone of Atic (Myc-DDK-tagged) - Mouse 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (Atic) 10 ug
$851.00
MR219425L4 Lenti ORF clone of Atic (mGFP-tagged) - Mouse 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (Atic) 10 ug
$851.00

Plasmid Map for MG219425

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG219425
Circular map for MG219425

file-alt Citations

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