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Slc5a3 (NM_017391) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG219414
Slc5a3 (tGFP-tagged) - Mouse solute carrier family 5 (inositol transporters) member 3 (Slc5a3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $857.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc5a3
Synonyms AA623876; BF642829; Smit1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219414 representing NM_017391
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCTGTACTGGAGGCAGCAGACATTGCCGTAGTGGCCCTGTATTTTATCCTGGTCATGTGCATTG
GCTTTTTTGCCATGTGGAAATCTAATAGAAGCACTGTGAGTGGATACTTCCTGGCCGGGCGCTCTATGAC
CTGGGTGGCGATTGGTGCCTCTCTGTTTGTGAGCAATATTGGGAGTGAACACTTCATTGGGCTGGCAGGA
TCTGGAGCAGCAAGTGGATTTGCAGTGGGCGCATGGGAATTCAATGCCTTACTGCTCTTGCAACTTCTGG
GATGGGTTTTCATCCCCATTTACATCCGGTCAGGGGTATATACCATGCCTGAATACTTGTCCAAGAGGTT
CGGTGGCCATAGGATTCAGGTCTATTTTGCAGCCTTGTCTCTGCTTCTCTATATCTTCACCAAGCTCTCA
GTGGATCTGTATTCAGGGGCCCTCTTTATCCAGGAGTCCTTGGGTTGGAACCTTTATGTGTCTGTCATCC
TGCTCATTGGCATGACTGCTCTGCTGACTGTCACCGGAGGCCTTGTTGCAGTGATCTACACAGACACTCT
GCAGGCCCTGCTCATGATCATTGGGGCGCTCACACTTATGGTTATTAGCATGGTGAAGATTGGAGGGTTT
GAGGAAGTTAAGAGAAGGTACATGTTGGCCTCACCTGATGTTGCTTCCATTTTGTTGAAGTACAACCTTT
CCAACACAAATGCTTGTATGGTCCATCCTAAGGCGAATGCCCTAAAAATGTTGAGAGACCCAACAGATGA
AGATGTTCCATGGCCTGGATTCATTCTTGGGCAAACCCCAGCCTCGGTATGGTACTGGTGTGCTGACCAA
GTCATCGTACAGAGGGTTCTAGCAGCCAAAAACATTGCTCATGCCAAAGGCTCCACTCTAATGGCTGGCT
TCTTGAAGCTTCTACCAATGTTTATCATAGTTGTACCAGGAATGATTTCCAGGATAGTGTTTGCTGATGA
GATAGCTTGCATCAACCCAGAACACTGCATGCAAGTGTGTGGAAGCAGAGCTGGCTGCTCCAATATTGCT
TACCCACGCCTGGTGATGACCTTGGTTCCTGTGGGCCTTCGGGGCCTGATGATGGCAGTGATGATTGCAG
CTCTGATGAGTGACTTGGACTCCATCTTTAACAGCGCCAGTACCATATTCACCCTCGATGTGTACAAACT
TATCCGCAAGAGTGCAAGCTCCCGGGAACTAATGATTGTGGGCAGGATATTTGTGGCTTTTATGGTTGTC
ATCAGCATAGCATGGGTGCCAATTATTGTGGAGATGCAAGGAGGCCAAATGTACCTTTACATCCAGGAGG
TGGCAGATTATCTGACTCCTCCCGTGGCAGCCCTCTTCCTTCTGGCAATTTTCTGGAAACGCTGCAATGA
GCAAGGGGCTTTTTATGGTGGAATGGCAGGCTTTGTTCTTGGAGCTGTCCGTTTGATACTGGCTTTTACC
TACCGTGCTCCTGAGTGTGATCAACCTGATAACAGGCCAGGCTTCATCAAAGACATCCATTATATGTACG
TGGCTACGGCATTATTTTGGATCACAGGACTCATTACTGTGATTGTTAGTCTTCTCACACCACCTCCCAC
AAAGGATCAGATTCGTACCACTACCTTTTGGTCAAAGAAGACCCTGGTGACAAAGGAGAGCTGTTCTCAG
AAAGACGAACCATACAAAATGCAAGAGAAGAGCATCCTGCAGTGCAGCGAGAATAGCGAAGTCATCAGCC
ATACTATTCCCAACGGGAAGTCTGAAGACAGCATTAAGGGACTGCAACCTGAAGACGTTAATCTGCTGGT
GACATGCCGAGAAGAGGGCAACCCAGTGGCTTCCATGGGTCATTCAGAGGCAGAAACACCAGTAGATGCT
TATTCCAATGGGCAAGCAGCTCTCATGGGTGAGAGAGAAAGAGAGAAGGAAACGGAAAATAGAAGCCGGT
ATTGGAAGTTCATAGACTGGTTTTGTGGCTTTAAGAGTAAAAGCCTTAGCAAGAGGAGCCTCAGAGACTT
GATGGATGAGGAGGCTGTGTGTTTACAAATGTTAGAAGAGACTCCACAAGTTAAAGTGATCCTAAACATT
GGACTTTTTGCTGTGTGTTCACTTGGAATTTTCATGTTTGTTTATTTCTCCTTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219414 representing NM_017391
Red=Cloning site Green=Tags(s)

MRAVLEAADIAVVALYFILVMCIGFFAMWKSNRSTVSGYFLAGRSMTWVAIGASLFVSNIGSEHFIGLAG
SGAASGFAVGAWEFNALLLLQLLGWVFIPIYIRSGVYTMPEYLSKRFGGHRIQVYFAALSLLLYIFTKLS
VDLYSGALFIQESLGWNLYVSVILLIGMTALLTVTGGLVAVIYTDTLQALLMIIGALTLMVISMVKIGGF
EEVKRRYMLASPDVASILLKYNLSNTNACMVHPKANALKMLRDPTDEDVPWPGFILGQTPASVWYWCADQ
VIVQRVLAAKNIAHAKGSTLMAGFLKLLPMFIIVVPGMISRIVFADEIACINPEHCMQVCGSRAGCSNIA
YPRLVMTLVPVGLRGLMMAVMIAALMSDLDSIFNSASTIFTLDVYKLIRKSASSRELMIVGRIFVAFMVV
ISIAWVPIIVEMQGGQMYLYIQEVADYLTPPVAALFLLAIFWKRCNEQGAFYGGMAGFVLGAVRLILAFT
YRAPECDQPDNRPGFIKDIHYMYVATALFWITGLITVIVSLLTPPPTKDQIRTTTFWSKKTLVTKESCSQ
KDEPYKMQEKSILQCSENSEVISHTIPNGKSEDSIKGLQPEDVNLLVTCREEGNPVASMGHSEAETPVDA
YSNGQAALMGEREREKETENRSRYWKFIDWFCGFKSKSLSKRSLRDLMDEEAVCLQMLEETPQVKVILNI
GLFAVCSLGIFMFVYFSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017391
ORF Size 2154 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017391.3, NP_059087.2
RefSeq Size 10918 bp
RefSeq ORF 2157 bp
Locus ID 53881
UniProt ID Q9JKZ2
Cytogenetics 16 C4
Summary Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function.UniProtKB/Swiss-Prot Function
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"NM_017391" in other vectors (4)
SKU Description Size Price
MC221007 Slc5a3 (untagged) - Mouse solute carrier family 5 (inositol transporters), member 3 (Slc5a3), (10ug) 10 ug
$724.00
MR219414 Slc5a3 (Myc-DDK-tagged) - Mouse solute carrier family 5 (inositol transporters), member 3 (Slc5a3) 10 ug
$589.00 $657.00
MR219414L3 Lenti ORF clone of Slc5a3 (Myc-DDK-tagged) - Mouse solute carrier family 5 (inositol transporters), member 3 (Slc5a3) 10 ug
$957.00
MR219414L4 Lenti ORF clone of Slc5a3 (mGFP-tagged) - Mouse solute carrier family 5 (inositol transporters), member 3 (Slc5a3) 10 ug
$957.00

Plasmid Map for MG219414

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG219414
Circular map for MG219414

file-alt Citations

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