Dgat2 (NM_026384) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG218673
Dgat2 (tGFP-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2), (10ug)
$886.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dgat2 |
| Synonyms | 0610010B06Rik; ARAT; DGAT-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_026384 |
| ORF Size | 1164 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_026384.3, NP_080660.1 |
| RefSeq Size | 2251 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 67800 |
| UniProt ID | Q9DCV3 |
| Cytogenetics | 7 E1 |
| Summary | Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_026384" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC210628 | Dgat2 (untagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2), (10ug) | 10 ug |
$686.00
|
|
| MR218673 | Dgat2 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) | 10 ug |
$686.00
|
|
| MR218673L3 | Lenti ORF clone of Dgat2 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) | 10 ug |
$986.00
|
|
| MR218673L4 | Lenti ORF clone of Dgat2 (mGFP-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) | 10 ug |
$986.00
|
|
Citations
Host triacylglycerols shape the lipidome of intracellular trypanosomes and modulate their growth
Gazos-Lopes, Martin, Dumoulin et al
PLoS Pathog. (2017) 13 (12), e1006800 DOI: 10.1371/journal.ppat.1006800
Gazos-Lopes, Martin, Dumoulin et al
PLoS Pathog. (2017) 13 (12), e1006800 DOI: 10.1371/journal.ppat.1006800
AI Summary: OriGene's Mouse cDNA ORF Clone of Dgat2 (NM_026384) was used to complement DGAT1/2-deficient mouse embryonic fibroblasts, enabling the study of host triacylglycerol synthesis in the interaction between host cells and intracellular Trypanosoma cruzi.
Snippet: … DGAT1/2-/- complementation with OriGene Mouse cDNA ORF Clone of Dgat2 (NM_026384) was performed according with the manufacturer’s protocol for the generation of stable transfectants
Chlamydia trachomatis regulates growth and development in response to host cell fatty acid availability in the absence of lipid droplets
Sharma, Recuero-Checa, Fan et al
Cell. Microbiol. (2017)
Sharma, Recuero-Checa, Fan et al
Cell. Microbiol. (2017)
Cited by 23
Showing 1-2 of 2 papers.
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