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Prodh (NM_011172) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG218491
Prodh (tGFP-tagged) - Mouse proline dehydrogenase (Prodh) nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $758.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prodh
Synonyms Pro-1; Pro1; Ym24d07
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG218491 representing NM_011172
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTCAAGCGCGTCTTCTTGCTGCGGTCGGTGGCACCACGCGTCGCTGCCCTCTCAACCAAACCGC
AAGCCCAGGAACAGCCTCCCGCGAGCCCTGAGGCTCTTCGGGGATGTGGGGCGGCCAAGGCTGTGCGGCC
GCCTGTGCCAGCCGTGGACTTCACCAACACGCAGGAGGCGTATCGCAGCCGGCGGAGTTGGGAGTTGGTG
CGCAACCTGCTAGTGCTGCGGCTGTGTGCGTCGCCGGTGCTGCTAGCGCACCACGAGCAGTTGTTCCAAG
TTGCCAGGAAGCTTCTGGGGCAAAGGATGTTCGAGAGATTGATGAAGATGACCTTCTATGGCCATTTTGT
GGCTGGCGAGGACCAGGAGTCTATCAGGCCTCTGATCCGGCACAACAAAGCCTTTGGTGTTGGCTTTATC
CTGGACTATGGAGTGGAGGAAGATCTGAGCCCTGAGGAGGCGGAGCGCAAAGAGATGGAGTCATGCACTT
CTGAAGCAGAGAGAGATGGCAGTGGAGCAAATAAGAGGGAGAAGCAGTATCAGGTGCACCCCGCCTTTGG
AGACCGCAGAGATGGTGTCATCAGTGCCCGCACCTACTTCTATGCCAATGAAGCCAAGTGTGACAACTAC
ATGGAGAACTTACTGCAGTGCATCAAGGCCTCAGGTGGAGCCAGTGATGGTGGTTTCTCAGCCATTAAGC
TCACTGCACTGGGGAGACCACAGTTTCTGCTGCAGTTCTCAGACGTGCTGACCAGGTGGAGACGGTTCTT
CCATCAAATGGCTGCAGAGCAGGGACAGGCTGGGCGTGCTGCTGTAGACACAAAGCTGGAGGTGGCGGTG
CTCCAGGACAGCATCGCAAAGATGGGCATCGCATCCAGGGCTGAGATTGAAGGGTGGTTCACGCCAGAGA
CGCTGGGAGTGTCTGGCACCGTGGACTTGCTGGACTGGAACAGCCTCATTGACAGCAGGACCCGGCTCTC
CAGGCACTTGGTGGTCCCCAATGTGCAGACTGGCCAGCTGGAGCCCCTGCTGTCACGGTTCACTGAGGAG
GAAGAGCAGCAGATGAAAAGGATGCTGCAGAGGATGGATGTACTGGCCAAGAAAGCAAAAGAGGCAGGTG
TGCGCCTGATGATTGATGCTGAGCAGAGCTACTTCCAACCAGCCATCAGCCGCCTGACCCTGGAGATGCA
GCGCAGGTTCAATGTGGATAAGCCGTTCATCTTCAACACATTCCAGTGCTACCTCAAGGATGCCTATGAC
AATGTGACCTTGGATATGGAACTGGCTCGCCGTGAGGGCTGGTGTTTTGGGGCCAAGCTGGTACGTGGTG
CATACATGGCCCAAGAGCGTGTCAGGGCAGCAGAGATCGGTTATGAGGACCCCATCAACCCTACATATGA
AGCCACCAATGCTATGTACCACAGGTGCCTTAACTATGTTCTGGAGGAGCTGAAGCACAGCACCAAGGCA
GAGGTGATGGTGGCTTCCCACAACGAGGACACCGTGCACTTCACGTTGTGCAGGATGAAGGAGATAGGCC
TGCATCCTGCTGATGGTCAGGTGTGCTTCGGACAGCTGCTGGGGATGTGTGACCAAATCAGCTTCCCACT
AGGCCAGGCAGGCTTTCCTGTGTACAAGTATGTGCCCTATGGCCCTGTGATGGAGGTACTCCCTTACCTG
TCCCGCCGTGCCCTGGAGAACAGCAGCATCATGAAGGGTGCTCAGCGAGAGAGGCAGCTGCTATGGCAGG
AGCTCCGCAGGCGGCTGCGCACTGGCAGCCTCTTCCACCATCCGGCC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG218491 representing NM_011172
Red=Cloning site Green=Tags(s)

MALKRVFLLRSVAPRVAALSTKPQAQEQPPASPEALRGCGAAKAVRPPVPAVDFTNTQEAYRSRRSWELV
RNLLVLRLCASPVLLAHHEQLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFI
LDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYANEAKCDNY
MENLLQCIKASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRRFFHQMAAEQGQAGRAAVDTKLEVAV
LQDSIAKMGIASRAEIEGWFTPETLGVSGTVDLLDWNSLIDSRTRLSRHLVVPNVQTGQLEPLLSRFTEE
EEQQMKRMLQRMDVLAKKAKEAGVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYD
NVTLDMELARREGWCFGAKLVRGAYMAQERVRAAEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKA
EVMVASHNEDTVHFTLCRMKEIGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYL
SRRALENSSIMKGAQRERQLLWQELRRRLRTGSLFHHPA

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_011172
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011172.2, NP_035302.2
RefSeq Size 2283 bp
RefSeq ORF 1800 bp
Locus ID 19125
UniProt ID Q9WU79
Cytogenetics 16 11.19 cM
Summary Converts proline to delta-1-pyrroline-5-carboxylate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011172" in other vectors (4)
SKU Description Size Price
MC219514 Prodh (untagged) - Mouse proline dehydrogenase (Prodh), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$614.00
MR218491 Prodh (Myc-DDK-tagged) - Mouse proline dehydrogenase (Prodh), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $558.00
MR218491L3 Lenti ORF clone of Prodh (Myc-DDK-tagged) - Mouse proline dehydrogenase (Prodh), nuclear gene encoding mitochondrial protein 10 ug
$858.00
MR218491L4 Lenti ORF clone of Prodh (mGFP-tagged) - Mouse proline dehydrogenase (Prodh), nuclear gene encoding mitochondrial protein 10 ug
$858.00

Plasmid Map for MG218491

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG218491
Circular map for MG218491

file-alt Citations

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