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Gpc5 (NM_175500) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG218095
Gpc5 (tGFP-tagged) - Mouse glypican 5 (Gpc5), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $733.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpc5
Synonyms A230034F01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG218095 representing NM_175500
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCCCGCACCTGGCGTCTGGGCTGGCGCTGTCTCCTTCTCCTGGCTCTCCTTGGATCTACCCGAA
GCGAGGGCGTGGAGAGCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGGCTTGGGGGAGCTGTCAAAGG
GCTGCCGGAGGCGCCGCGGGCAGGACCTGATCTTCAGGTTTGCCTATCCAAAAACCCAACGTGCTGTACC
AGGAAGATGGAGGAGAGGTACCAGATTGCAGCTCGGCAAGATTTGCAGCAGGTGCTTCAGACATCCAGCT
CAACATTAAAGTTGCTAATATCTCGAAATGCAGCTGCTTTTCAAGAGACCCTGGAAACCCTCATCAGACA
AGCAGAGAATTACACCAGTATCCTTTTCTGCAACACCTACCGGAACATGGCCTTGGAGGCTGCTGCTTCC
ATTCAGGAATTTTTCACGGATGTTGGACTCTATTTATTTGGTGCGGATGTTAATCCCGAGGAATTTGTAA
ACAGATTCTTTGACAGTCTTTTTCCTCTGGTCTACAACCATCTTATAAACCCTGGTGTGACTGACAGTTC
TCTGCAATACTCAGAATGCATTCGAATGGCCCGCCAGGATGTTAGTCCATTTGGCAACATTCCCAAAAGA
GTCATGGGGCAGATGGGGAGGTCGCTGCTGCCTGGCCGCACATTCTTGCAGGCGCTTAATCTGGGCATTG
AGGTCATCAACACCACGGACCACATACACTTCTCCAAGGAGTGCAGTAGGGCCCTCCTGAAGATGCAGTA
TTGTCCTCACTGCCAGAGCCTGATGCTCAGTAAGCCCTGTATGGGGTACTGCCTCAATGTCATCAGGGGC
TGCCTGGCCCACATGACTGAGCTTAATCCACACTGGCATGCTTATATCCGGTCTTTGGAAGAGTTGTCAG
ATGCGATGCACGGAACCTACGACGTTGAACACGTGCTCCTGAACTTCCACTTGCTTGTTAATGATGCCGT
GTTGCAGGCTCACCTCAATGGGCAGAAATTATTGGATCAGGTGAATACGATTTGTGGCCATCCAGTGAGA
ACACCAACACAGAGCCCCCGCTGTACTTTTGACCCAAGCAAAGAGAAACATGGAATGAAGATCTCTGCAA
GGAATGGTGAAGAGACACTTGCCAACAGAAGAAAAGAATTCATCAATAGCCTTAGGCTGCATGGGTCCTT
CTATGGTGGCCTGGCTGACCAGCTTTGTGTTAATGAATTGGCTGCCCCTGAAGGAAGGCCCTGCTGGAAT
GGGGAGGAAATAGTCAAAAGCTATGCTCAGCGTGTGGTTGGGAATGGAATCAAAGCCCAGTCTGCAAATC
CTGAAGTCAGAGTCAGAGGAACCGATCCTGTGGTAAATCAGATCATTGATAAACTGAAGCATGTCATCCA
GTTGTTACGGGGGAGATCACCCAAGCCCAACAAGTGGGAGCTCCTTCAGCCAGGCAGTGGAGGCGGCATG
CTTGAGAACTCTAGTGGAGACTGTGATGACGAAGATGGCTGTGGAGGATCAGGAAGCGGAGAAGTAAAGA
GGACCCTGAAGATCACAAACTGGATGCCAGACAGCATGAACTTCAGTGATGTGAAGCAAGTCCATCGAGC
AGACCATGGAAGTACTCTGGATACAACCAGCACCGGATGTGCGTCAGGAACTGAGTCTATGGCACTCCCT
CTGATGGGGACACTGATGTTTCTTCCCTGGCTTTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG218095 representing NM_175500
Red=Cloning site Green=Tags(s)

MDARTWRLGWRCLLLLALLGSTRSEGVESCEEVRKLFQWRLGGAVKGLPEAPRAGPDLQVCLSKNPTCCT
RKMEERYQIAARQDLQQVLQTSSSTLKLLISRNAAAFQETLETLIRQAENYTSILFCNTYRNMALEAAAS
IQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLQYSECIRMARQDVSPFGNIPKR
VMGQMGRSLLPGRTFLQALNLGIEVINTTDHIHFSKECSRALLKMQYCPHCQSLMLSKPCMGYCLNVIRG
CLAHMTELNPHWHAYIRSLEELSDAMHGTYDVEHVLLNFHLLVNDAVLQAHLNGQKLLDQVNTICGHPVR
TPTQSPRCTFDPSKEKHGMKISARNGEETLANRRKEFINSLRLHGSFYGGLADQLCVNELAAPEGRPCWN
GEEIVKSYAQRVVGNGIKAQSANPEVRVRGTDPVVNQIIDKLKHVIQLLRGRSPKPNKWELLQPGSGGGM
LENSSGDCDDEDGCGGSGSGEVKRTLKITNWMPDSMNFSDVKQVHRADHGSTLDTTSTGCASGTESMALP
LMGTLMFLPWLW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175500
ORF Size 1716 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175500.4, NP_780709.1
RefSeq Size 2885 bp
RefSeq ORF 1719 bp
Locus ID 103978
UniProt ID Q8CAL5
Cytogenetics 14 E4
Summary Cell surface proteoglycan that bears heparan sulfate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175500" in other vectors (4)
SKU Description Size Price
MC219210 Gpc5 (untagged) - Mouse glypican 5 (Gpc5), (10ug) 10 ug
$587.00
MR218095 Gpc5 (Myc-DDK-tagged) - Mouse glypican 5 (Gpc5) 10 ug
$289.00 $533.00
MR218095L3 Lenti ORF clone of Gpc5 (Myc-DDK-tagged) - Mouse glypican 5 (Gpc5) 10 ug
$833.00
MR218095L4 Lenti ORF clone of Gpc5 (mGFP-tagged) - Mouse glypican 5 (Gpc5) 10 ug
$833.00

Plasmid Map for MG218095

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG218095
Circular map for MG218095

file-alt Citations

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