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Lrfn2 (NM_027452) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG218081
Lrfn2 (tGFP-tagged) - Mouse leucine rich repeat and fibronectin type III domain containing 2 (Lrfn2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $922.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lrfn2
Synonyms 5730420O05Rik; mKIAA1246; SALM1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG218081 representing NM_027452
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTCTGCTTGGTGGGCTGCTGGCTTTTGGCATGGCGTTTGCTGTGGTCGATGCCTGCCCCAAGT
ACTGCGTCTGCCAGAATCTGTCTGAGTCACTGGGGACCCTGTGTCCCTCCAAGGGGCTCCTCTTTGTGCC
CCCTGACATTGACCGGAGGACGGTGGAGCTGCGTCTGGGTGGCAACTTCATCATCCACATTGGCCGCCAA
GACTTTGCCAACATGACAGGGCTGGTGGATCTGACCTTGTCCAGGAACACCATCAGCCATATCCAGCCCT
TCTCCTTCCTGGACCTTGAGAGCCTCCGCTCCCTGCACCTTGACAGCAACCGGCTACCTAGCCTTGGGGA
GGACACACTCCGGGGTCTGGTCAACCTGCAACACCTTATTGTGAACAATAACCAGCTGGGTGGCATCGCT
GATGATGCCTTTGAAGACTTCTTGCTGACTTTAGAAGACCTGGACCTATCTTACAACAACCTCCACGGAC
TTCCCTGGGATTCCGTACGGCGCATGGTCAACCTCCATCAGCTGAGTCTGGACCACAATCTGCTGGACCA
CATTGCTGAGGGCACTTTTGCAGACCTGCAGAAACTGGCCCGCCTGGACCTCACATCCAATCGGCTGCAG
AAACTTCCCCCGGACCCCATCTTTGCCCGCTCCCAGGCTTCCTTGCTAACTGCCACGCCCTTTGCCCCAC
CCCTGTCTTTTAGTTTTGGAGGGAACCCGCTGCACTGCAATTGTGAGCTTCTCTGGCTGCGGAGGCTGGA
GAGGGATGATGACCTGGAGACCTGTGGATCCCCCGGAAGTCTCAAGGGTCGCTACTTTTGGCATATTCGT
GAGGAGGAGTTTGTGTGTGAGCCGCCTCTCATCACCCAGCACACACACAAGCTGCTGGTTCTGGAGGGCC
AGGCAGCCACTCTTAAGTGCAAGGCCATTGGGGACCCCAGCCCTTTGATCCACTGGGTTGCCCCCGATGA
CCGCTTGGTGGGAAACTCTTCCAGGACTGCTGTATATGACAATGGCACCCTGGACATTCTAATTACCACC
TCTCAGGACAGTGGACCCTTTACCTGCATCGCAGCCAATGCAGCAGGAGAGGCTACGGCCACTGTAGAGG
TCTCCATTGTGCAGCTCCCACACCTTAGTAACAGCACTAGCCGGATGGCACCCCCCAAGTCTCGCCTTTC
GGACATCACAGGTTCCAGCAAGACCAGCCGGGGAGGGGGAGGTAGTGGGGCTGGGGAGCCTCCCAAAAGC
ACCCCAGAGAGGGCTGTGCTTGTGTCCGATGTTACCACCACATCTGCCCTGGTCAAGTGGTCTGTCAGCA
AGTCAGCACCCAGGGTGAAGATGTATCAGCTGCAGTACAACTGTTCTGATGATGAGGTGCTGATCTACAG
GATGATCCCAGCCTCTAACAAGGCCTTCGTGGTCAACAACCTGGTGTCCGGAACGGGCTACGACTTGTGC
GTACTGGCTATGTGGGACGACACAGCCACGACGCTCACAGCCACCAACATTGTGGGCTGTGCCCAGTTCT
TCACCAAGGCCGACTACCCACAATGCCAGTCTATGCACAGTCAGATCCTGGGGGGCACCATGATTCTGGT
CATCGGAGGTATCATTGTGGCCACACTGCTGGTCTTCATTGTAATCCTCATGGTGCGGTACAAGGTCTGC
AACCACGACACCCCAGGAAAAATGGCAGCCGCCACTGTCAGCAATGTGTACTCGCAGACCAATGGGTCTC
AGCCTCCACCTCTGGGTGGGATACCCGTTGGCCAGCTTCCTCAGGCACCACCCAAAGTTGTGGTGCGAAA
TGAGTTGATGGACTTCAGTACCAGCCTGGCCAGGGCCTGTGACTCCTCTTCTTCCAGCTCACTGGGCAGC
GGGGAAGCCGCGGGGCTGGGCCGGGGTCCCTGGAGGCTCCCACCCCCAGCTCCTCGTCCCAAGCCCAGCC
TTGACCGCTTGATGGGAGCCTTTGCCTCCCTGGACCTCAAGAGTCAAAGGAAAGAAGAACTTCTAGACTC
CAGGACTCCAGCAGGCAGAGGGGCAGGGACATCATCCAGAGGCCACCACTCAGACCGAGAGCCACTGCTG
GGGCCCCCGGCCACCCGTGCCAGGAGTCTTCTCCCTTTGCCCCTGGAGGGCAAGGCCAAACGGAGCCACT
CTTTTGACATGGGGGACTTTGCAGCTGCAGCTGCAGCTGTTCCTGGTGGCTACAGTCCCCCTAGGCGGGT
CTCAAACATCTGGACGAAGCGCAGCCTGTCTGTCAATGGCATGCTCTTGCCCTTTGAGGAGAGTGACCTG
GTGGGAGCCCGGGGGACATTTGGCAGCTCAGAGTGGGTAATGGAAAGTACTGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG218081 representing NM_027452
Red=Cloning site Green=Tags(s)

METLLGGLLAFGMAFAVVDACPKYCVCQNLSESLGTLCPSKGLLFVPPDIDRRTVELRLGGNFIIHIGRQ
DFANMTGLVDLTLSRNTISHIQPFSFLDLESLRSLHLDSNRLPSLGEDTLRGLVNLQHLIVNNNQLGGIA
DDAFEDFLLTLEDLDLSYNNLHGLPWDSVRRMVNLHQLSLDHNLLDHIAEGTFADLQKLARLDLTSNRLQ
KLPPDPIFARSQASLLTATPFAPPLSFSFGGNPLHCNCELLWLRRLERDDDLETCGSPGSLKGRYFWHIR
EEEFVCEPPLITQHTHKLLVLEGQAATLKCKAIGDPSPLIHWVAPDDRLVGNSSRTAVYDNGTLDILITT
SQDSGPFTCIAANAAGEATATVEVSIVQLPHLSNSTSRMAPPKSRLSDITGSSKTSRGGGGSGAGEPPKS
TPERAVLVSDVTTTSALVKWSVSKSAPRVKMYQLQYNCSDDEVLIYRMIPASNKAFVVNNLVSGTGYDLC
VLAMWDDTATTLTATNIVGCAQFFTKADYPQCQSMHSQILGGTMILVIGGIIVATLLVFIVILMVRYKVC
NHDTPGKMAAATVSNVYSQTNGSQPPPLGGIPVGQLPQAPPKVVVRNELMDFSTSLARACDSSSSSSLGS
GEAAGLGRGPWRLPPPAPRPKPSLDRLMGAFASLDLKSQRKEELLDSRTPAGRGAGTSSRGHHSDREPLL
GPPATRARSLLPLPLEGKAKRSHSFDMGDFAAAAAAVPGGYSPPRRVSNIWTKRSLSVNGMLLPFEESDL
VGARGTFGSSEWVMESTV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027452
ORF Size 2364 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027452.3, NP_081728.2
RefSeq Size 3001 bp
RefSeq ORF 2367 bp
Locus ID 70530
UniProt ID Q80TG9
Cytogenetics 17 C
Summary Promotes neurite outgrowth in hippocampal neurons. Enhances the cell surface expression of 2 NMDA receptor subunits GRIN1 and GRIN2A (By similarity). May play a role in redistributing DLG4 to the cell periphery.UniProtKB/Swiss-Prot Function
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"NM_027452" in other vectors (4)
SKU Description Size Price
MC221640 Lrfn2 (untagged) - Mouse leucine rich repeat and fibronectin type III domain containing 2 (Lrfn2), (10ug) 10 ug
$795.00
MR218081 Lrfn2 (Myc-DDK-tagged) - Mouse leucine rich repeat and fibronectin type III domain containing 2 (Lrfn2) 10 ug
$589.00 $722.00
MR218081L3 Lenti ORF clone of Lrfn2 (Myc-DDK-tagged) - Mouse leucine rich repeat and fibronectin type III domain containing 2 (Lrfn2) 10 ug
$1,022.00
MR218081L4 Lenti ORF clone of Lrfn2 (mGFP-tagged) - Mouse leucine rich repeat and fibronectin type III domain containing 2 (Lrfn2) 10 ug
$1,022.00

Plasmid Map for MG218081

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG218081
Circular map for MG218081

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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