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Gnl3l (NM_198110) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217821
Gnl3l (tGFP-tagged) - Mouse guanine nucleotide binding protein-like 3 (nucleolar)-like (Gnl3l) transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $738.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gnl3l
Synonyms BC020354
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217821 representing NM_198110
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAAGATTAGACACAAAAACAAAAAACCAGGTAAAGGTTCCAAAGGCTGTAAGAAGCCTGCAAGGC
AAAATGGGAAGAAAGTAACCTCCAGACCATCATCTGCTCCCCAGATTGTTCATGGCAATGACCATGCCAG
TCGCGAGGCCGAATTAAAGAAGAAAAGGGTCGAGGAGATGAGGGAGAAGCAGCAAGTTGCCCGGGAGCAA
GAGAGACAGAGACACAGGACCATGGAGAGCTATTGTCAGGATGTCCTGAAACGTCAGCAGGAATTTGAAC
AAAAGGAGGAAGTTTTGCAGGAATTAAACATGTTTCCTCAGTTGGATGATGAGGCCACAAGGAAGGCCTA
TTACAAGGAATTCCGGAAGGTGGTAGAGTACTCTGATGTGATTCTGGAAGTCCTAGATGCCAGAGACCCA
TTGGGCTGCCGCTGTTTCCAGATGGAGGAGACTGTCCTTCGTGCAGAAGGCAACAAGAAGCTGGTCTTGG
TCTTAAATAAGATAGATCTCGTTCCCAAGGAGATTGTGGAAAAGTGGCTGGAATACCTTCTCAATGAACT
GCCAACTGTGGCTTTCAAGGCCAGCACCCAGCATCATCAGGTCAAAAACTTGACTCGTTGTAAAGTTCCA
GTGGACCAGGCCTCTGAGTCGCTTTTGAAAAGCAGAGCCTGCTTTGGAGCCGAAAATCTCATGAGGGTCC
TGGGGAACTATTGTCGCCTGGGGGAAGTGCGTGGCCACATTCGTGTGGGTGTTGTAGGCCTTCCCAATGT
GGGGAAGAGCAGTCTGATCAATAGCCTGAAGCGCAGCCGTGCTTGTAGTGTGGGAGCTGTTCCTGGTGTC
ACAAAATTCATGCAGGAGGTCTACCTAGACAAGTTTATCAGGCTTCTGGATGCACCAGGCATTGTCCCAG
GACCCAATTCAGAGGTGGGCACCATCCTGCGTAATTGCATCCATGTGCAGAAGCTGGCAGACCCTGTGAC
CCCGGTGGAGACCATCCTTCAGCGCTGCAACCTGGAGGAGATTTCCAGCTACTATGGTGTATCTGGATTC
CAGACGACTGAGCACTTTCTGACTGCAGTGGCCCATCGCTTGGGAAAGAAGAAGAAGGGAGGTGTATATA
GTCAGGAACAGGCTGCCAAAGCTGTGCTGGCTGACTGGGTGAGTGGGAAGATCAGCTTCTATACACTACC
ACCGCCCACTCACACTCTGCCCACCCATCTCAGTGCTGAGATTGTTAAGGAGATGACTGAGGTCTTTGAT
ATAGAAGATACTGAGCACGCCAATGAAGACACCATGGAATGCTTAGCTGTGGGAGAATCCGATGAGCTGT
TGGGTGACATGGACCCACAAGAAATGGAGGTCAGGTGGCTCCATTCTCCACTGGTGAAAATAGCAGATGC
TATTGAAAATAGAAGCACCGTGTATAAGATTGGAAATCTCACTGGGTATTGTACCAAACCAAACCGTAAT
CAGATGGGGTGGCCTAAACGCAATGTGGACCACCATTGCCCCCAAAATAACCGTGTAGTAGAGGTCAGTT
CTGTGGACCGCCGCCCGATGTTGCAGAGGATCCTGGAGACAGACCCACTTCAGCAAGGCCAGGCTCTGGC
ATCTGCCTTGAAGAATAAGAAAAAATTGCAGAAGCGTTCAGATAAAATCGCCACTAAGTTGTCTGACTCC
ATGATGTCCATGCTTGACCTCTCTGGCAACTCCGATGACTGTGCAGGTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217821 representing NM_198110
Red=Cloning site Green=Tags(s)

MMKIRHKNKKPGKGSKGCKKPARQNGKKVTSRPSSAPQIVHGNDHASREAELKKKRVEEMREKQQVAREQ
ERQRHRTMESYCQDVLKRQQEFEQKEEVLQELNMFPQLDDEATRKAYYKEFRKVVEYSDVILEVLDARDP
LGCRCFQMEETVLRAEGNKKLVLVLNKIDLVPKEIVEKWLEYLLNELPTVAFKASTQHHQVKNLTRCKVP
VDQASESLLKSRACFGAENLMRVLGNYCRLGEVRGHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGV
TKFMQEVYLDKFIRLLDAPGIVPGPNSEVGTILRNCIHVQKLADPVTPVETILQRCNLEEISSYYGVSGF
QTTEHFLTAVAHRLGKKKKGGVYSQEQAAKAVLADWVSGKISFYTLPPPTHTLPTHLSAEIVKEMTEVFD
IEDTEHANEDTMECLAVGESDELLGDMDPQEMEVRWLHSPLVKIADAIENRSTVYKIGNLTGYCTKPNRN
QMGWPKRNVDHHCPQNNRVVEVSSVDRRPMLQRILETDPLQQGQALASALKNKKKLQKRSDKIATKLSDS
MMSMLDLSGNSDDCAGD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198110
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198110.2, NP_932778.1
RefSeq Size 4877 bp
RefSeq ORF 1734 bp
Locus ID 237107
UniProt ID Q6PGG6
Cytogenetics X F3
Summary Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_198110" in other vectors (4)
SKU Description Size Price
MC219230 Gnl3l (untagged) - Mouse guanine nucleotide binding protein-like 3 (nucleolar)-like (Gnl3l), transcript variant 1, (10ug) 10 ug
$592.00
MR217821 Gnl3l (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein-like 3 (nucleolar)-like (Gnl3l), transcript variant 1 10 ug
$289.00 $538.00
MR217821L3 Lenti ORF clone of Gnl3l (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein-like 3 (nucleolar)-like (Gnl3l), transcript variant 1 10 ug
$838.00
MR217821L4 Lenti ORF clone of Gnl3l (mGFP-tagged) - Mouse guanine nucleotide binding protein-like 3 (nucleolar)-like (Gnl3l), transcript variant 1 10 ug
$838.00

Plasmid Map for MG217821

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG217821
Circular map for MG217821

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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