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Lrrc8e (NM_028175) Mouse Tagged ORF Clone

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SKU
MG217110
Lrrc8e (tGFP-tagged) - Mouse leucine rich repeat containing 8 family member E (Lrrc8e), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $928.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lrrc8e
Synonyms 1810049O03Rik; C87354
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217110 representing NM_028175
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCCAGTGGCGGAGTTCAAGCAGTTCACAGAGCAGCAGCCTGCATTCAAGGTGCTCAAACCCTGGT
GGGATGTTCTGGCTGAGTACCTCACCATGGCTATGCTCATGATCGGGGTATTCGGGTGCACCCTCCAGGT
GACACAGGACAAGATCATCTGCTTGCCTAGCCACGAATCTCGTGAGAACATATCGGGGGCTCCATGCCAG
CAGCTTTTGCCTCAAGGAATCTCTGAGCAGATGGGGGGTCTCCGAGAGCTCAGTGGCCTCAAAAACAACC
TAGACCTCCAGCAGTATAGCTTTATCAACCAGCTCTGCTATGAGACTGCCCTCCACTGGTATGCCAAATA
CTTCCCCTACTTGGTGGTTATCCATACTCTCATCTTTATGGTCTGTACCAGCTTTTGGTTCAAATTCCCT
GGTACCAGCTCCAAAATAGAACACTTCATCTCCATCCTGGGCAAGTGCTTTGATTCTCCATGGACCACTC
GGGCCTTGTCCGAGGTGTCTGGAGAAAATCACAAGGGCCCAGCTTCTGGACGGGCCATGGTGACCACGGT
AACCACCACAGGGGCAGGGTCGGGGAAGGTGGGTGAAGGTGAGAAGGAGAAAGTGCTCATTGAGCCCGAA
AAGGTGGTGTCTGAGCCTCCAGTTGTTACTCTGCTGGATAAGAAGGAGGGCGAGCAAGCGAAGGCCCTAT
TCGAGAAGGTCAAAAAGTTCCGAGTGCACGTGGAAGAGGGTGACATCCTCTATTCCATGTACATCCGGCA
GACAGTGCTCAAAGTGTGTAAGTTTTTTGCCATCCTGGTTTACAACCTGATCTATGTGGAGAAGATCAGC
TTTCTGGTGGCCTGTAGGGTGGAGACATCAGAGATCACGGGGTATGCCAGTTTCTGCTGCAACCACACCA
AGGCCCACCTCTTTTCTAAGCTGGCCTTCTGCTATATTTCCTTTGTGTGTGTCTATGGCATCACCTGCCT
GTATACACTCTACTGGCTCTTCCATAGGCCCCTAAAAGAGTACTCCTTCCGTTCTGTGCGTGAGGAGACA
GGGATGAACGATATCCCGGATGTTAAGAATGACTTTGCCTTCATGCTCCACCTCATTGACCAGTACGACT
CCCTCTATTCCAAACGCTTTGCAGTCTTCCTCTCTGAAGTCAGTGAGAGCCGCCTTAAGCAACTCAACCT
CAACCATGAGTGGACACCTGAGAAGCTGAGGCAGAAGCTGCAGCGTAACATGCGTGGCCGGCTGGAGCTG
AGCCTCTGCATGCTCCCTGGACTACCTGACACCGTCTTTGAGCTCAGCGAGGTGGAGGCACTCAGGCTGG
AGGCCATCTGTGACATCTCCTTTCCCCCTGGGCTCTCCCAGCTTGTGAACCTTCAGGAGCTCAGCTTGCT
TCACTCACCAGCCAGGCTGCCCTTTTCATCACAGATCTTCCTGCGAGATCGCCTGAAGGTGATCTGTGTT
AAATTCGAGGAGCTCCGAGAGGTACCCCTCTGGGTGTTTGGGCTTCGAGGCTTAGAAGAACTGCACCTGG
AAGGACTCTTTCCCCCAGAAATGGCTCGGGGTGCCACCCTGGAGAGCCTCCGGGAGCTTAAACAGCTCAA
GGTACTGTCTCTACGTAGCAATGCAGGGAAGGTGCCAGCCAGTGTGACTGATGTGGCCGGGCACCTTCAG
CGACTCAGCCTGCACAATGATGGGGCTCGTTTGCTTGCCCTAAACAGCCTCAAGAAGCTGGCAGTGTTAC
GGGAGCTGGAGCTGGTGGCCTGTGGGCTGGAACGTATCCCCCATGCAATCTTCAGCCTGGGGGCTCTGCA
GGAACTTGACCTTAAGGACAACCACCTTCGGTCTATCGAGGAGATCCTCAGCTTCCAGCACTGTCGGAAG
CTGGTCACTCTCAGGCTGTGGCACAACCAGATTGCCTATGTGCCCGAGCATGTGAGAAAGCTCCGGAGCC
TGGAGCAGCTCTACCTCAGTCACAACAAGCTGGAGACTCTGCCCACCCAGCTGGGCCAGTGCTTTGGCCT
CCGCCTGCTGGATTTATCCCACAACGGGCTTCGTTCCCTGCCACCTGAGTTGGGCCTTCTCCAGAGCCTC
CAGCATCTAGCCCTCTCCTACAACGCACTCGAGAGTCTGCCCGATGAATTGTTCTTCTGCCACAAGCTGC
GGACGTTACTCTTGGGCTACGACCACCTAACTCAGCTCTCACCTGACGTGGCTGCCCTCCAGGCCCTCAG
CCGTCTGGGGCTCAAGGGTAACAGGTTGGAGACACTGCCAGAAGAACTTGGTGACTGCAAGGGGCTGAAG
AAGTCGGGGCTGCTGGCGGAGGACACCCTTTATGAGGGACTGCCTGCAGAAGTTCGGGAAAAGATGGAGG
AGGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217110 representing NM_028175
Red=Cloning site Green=Tags(s)

MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTMAMLMIGVFGCTLQVTQDKIICLPSHESRENISGAPCQ
QLLPQGISEQMGGLRELSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFP
GTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPASGRAMVTTVTTTGAGSGKVGEGEKEKVLIEPE
KVVSEPPVVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLIYVEKIS
FLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLFHRPLKEYSFRSVREET
GMNDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNMRGRLEL
SLCMLPGLPDTVFELSEVEALRLEAICDISFPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICV
KFEELREVPLWVFGLRGLEELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQ
RLSLHNDGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILSFQHCRK
LVTLRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPTQLGQCFGLRLLDLSHNGLRSLPPELGLLQSL
QHLALSYNALESLPDELFFCHKLRTLLLGYDHLTQLSPDVAALQALSRLGLKGNRLETLPEELGDCKGLK
KSGLLAEDTLYEGLPAEVREKMEEE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028175
ORF Size 2385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028175.1
RefSeq Size 3878 bp
RefSeq ORF 2388 bp
Locus ID 72267
UniProt ID Q66JT1
Cytogenetics 8 A1.1
Summary Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Mediates efflux of amino acids, such as aspartate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028175" in other vectors (3)
SKU Description Size Price
MR217110 Lrrc8e (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8 family, member E (Lrrc8e) 10 ug
$589.00 $728.00
MR217110L3 Lenti ORF clone of Lrrc8e (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8 family, member E (Lrrc8e) 10 ug
$1,028.00
MR217110L4 Lenti ORF clone of Lrrc8e (mGFP-tagged) - Mouse leucine rich repeat containing 8 family, member E (Lrrc8e) 10 ug
$1,028.00

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