Homer2 (NM_011983) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG217093
Homer2 (tGFP-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2) transcript variant 1, (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Homer2
Synonyms 9330120H11Rik; AW539445; CPD; Vesl-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217093 representing NM_011983
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGAACAGCCCATCTTCACCACGCGAGCGCACGTCTTCCAGATTGACCCCAGCACCAAGAAGAACT
GGGTGCCGGCAAGCAAGCAGGCCGTCACGGTTTCCTACTTCTATGATGTCACCAGGAACAGCTATCGGAT
CATCAGTGTGGATGGAGCCAAGGTGATCATAAACAGCACTATCACCCCGAACATGACTTTCACCAAAACG
TCACAGAAGTTCGGGCAGTGGGCTGACAGCAGAGCCAACACCGTGTTCGGTTTGGGATTCTCCTCCGAGC
TGCAGCTCACGAAGTTTGCAGAGAAGTTCCAGGAGGTAAGAGAAGCTGCCAGGCTAGCCAGAGACAAGTC
CCAGGAGAAAACCGAGACCTCCAGCAATCATTCCCAAGAATCTGGGTGTGAAACCCCGTCTTCCACTCAG
GCATCCAGCGTCAATGGCACAGACGACGAAAAGGCCTCTCACGCGAGCCCAGCCGACACTCACCTCAAGT
CTGAGAATGACAAGCTGAAGATCGCGCTGACACAGAGTGCTGCCAATGTGAAGAAGTGGGAGATGGAGCT
GCAGACCCTGCGGGAGAGCAACGCCCGGCTGACCACGGCACTGCAGGAGTCGGCGGCCAGCGTGGAGCAG
TGGAAGCGGCAGTTCTCCATCTGCAGGGACGAGAATGACAGGCTCCGCAGCAAGATCGAGGAGCTGGAAG
AACAGTGCAGCGAGATAAACAGGGAGAAGGAGAAGAACACACAGCTGAAGAGGAGGATCGAGGAGCTGGA
GTCAGAGGTCCGAGACAAGGAGATGGAGTTGAAAGATCTCCGAAAACAGAGTGAAATCATACCTCAGCTC
ATGTCCGAGTGTGAATATGTCTCTGAGAAGTTAGAGGCGGCCGAAAGAGACAATCAAAACTTGGAAGACA
AAGTGCGGTCTCTAAAGACAGACATCGAGGAGAGTAAATACCGACAGCGCCACCTGAAGGGGGAGCTGAA
GAGCTTCCTTGAGGTGCTGGATGGAAAGATCGACGACCTCCATGACTTCCGTAGAGGACTCTCCAAGTTA
GGCACAGATAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217093 representing NM_011983
Red=Cloning site Green=Tags(s)

MGEQPIFTTRAHVFQIDPSTKKNWVPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKT
SQKFGQWADSRANTVFGLGFSSELQLTKFAEKFQEVREAARLARDKSQEKTETSSNHSQESGCETPSSTQ
ASSVNGTDDEKASHASPADTHLKSENDKLKIALTQSAANVKKWEMELQTLRESNARLTTALQESAASVEQ
WKRQFSICRDENDRLRSKIEELEEQCSEINREKEKNTQLKRRIEELESEVRDKEMELKDLRKQSEIIPQL
MSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKGELKSFLEVLDGKIDDLHDFRRGLSKL
GTDN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011983
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011983.2, NP_036113.1
RefSeq Size 10999 bp
RefSeq ORF 1065 bp
Locus ID 26557
UniProt ID Q9QWW1
Cytogenetics 7 D3
Summary Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2 (By similarity). Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses (By similarity) Required for normal hearing (PubMed:25816005). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011983" in other vectors (4)
SKU Description Size Price
MC209737 Homer2 (untagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1, (10ug) 10 ug
$503.00
MR217093 Homer2 (Myc-DDK-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 10 ug
$289.00 $457.00
MR217093L3 Lenti ORF clone of Homer2 (Myc-DDK-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 10 ug
$757.00
MR217093L4 Lenti ORF clone of Homer2 (mGFP-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.