Homer2 (NM_011983) Mouse Tagged ORF Clone
- TrueORF®
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Homer2 |
| Synonyms | 9330120H11Rik; AW539445; CPD; Vesl-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011983 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011983.2, NP_036113.1 |
| RefSeq Size | 10999 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 26557 |
| UniProt ID | Q9QWW1 |
| Cytogenetics | 7 D3 |
| Summary | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2 (By similarity). Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses (By similarity) Required for normal hearing (PubMed:25816005). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011983" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209737 | Homer2 (untagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1, (10ug) | 10 ug |
$503.00
|
|
| MR217093 | Homer2 (Myc-DDK-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 | 10 ug |
$289.00
$457.00
|
|
| MR217093L3 | Lenti ORF clone of Homer2 (Myc-DDK-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 | 10 ug |
$757.00
|
|
| MR217093L4 | Lenti ORF clone of Homer2 (mGFP-tagged) - Mouse homer homolog 2 (Drosophila) (Homer2), transcript variant 1 | 10 ug |
$757.00
|
|
Plasmid Map for MG217093
Download sequence for
Cloning scheme for MG217093
Circular map for MG217093
Citations
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