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Srgap2 (NM_001081011) Mouse Tagged ORF Clone

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SKU
MG217088
Srgap2 (tGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,132.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Srgap2
Synonyms 9930124L22Rik; AI448945; FBP2; Fnbp2; srGAP3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217088 representing NM_001081011
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATCGCAGAGTACGATACTCAGGTCAAAGAGA
TTAGGGCTCAGCTCACAGAGCAGATGAAATGCCTAGATCAGCAATGTGAGCTCCGGGTGCAGCTGTTGCA
GGACCTGCAGGACTTCTTCCGCAAGAAGGCTGAGATTGAAATGGACTACTCTCGAAACCTGGAGAAGCTA
GCCGAGCGCTTTCTAGCCAAGACACGAAGCACCAAGGACCAGCAATTTAAGAAGGACCAGAATGTTCTTT
CTCCAGTCAACTGCTGGAACCTCCTCTTAAACCAAGTGAAGCGGGAGAGCAGGGATCATACCACCCTGAG
TGACATCTACCTGAATAATATAATTCCTCGATTTGTTCAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA
AAGAGTAAAGAAGTTGGCCAGCAACTCCAAGATGATTTGATGAAAGTCCTGAACGAGCTTTACTCGGTCA
TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTGAAGGAAGCAGAGAAACA
AGAAGAGAAGCAAATTGGCAAATCAGTAAAGCAAGAGGACCGGCAGACCCCTCGCTCCCCTGACTCCACA
GCCAATGTCCGCATTGAGGAGAAGCATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA
AGCGACAAGCCAAGTACACAGAAAATAAGCTGAAGGCCATTAAAGCCCGGAATGAGTATTTATTGGCTTT
GGAGGCAACCAATGCATCTGTCTTCAAGTACTATATCCATGACCTGTCTGATATTATTGATCAATGTTGT
GACCTAGGCTACCATGCTAGCCTGAACCGGGCTCTACGCACTTTCCTATCTGCTGAATTAAATCTGGAAC
AGTCAAAACATGAAGGTCTGGATGCTATTGAAAATGCAGTAGAAAACCTAGATGCCACCAGTGACAAGCA
ACGGCTCATGGAGATGTACAACAATGTTTTTTGCCCCCCTATGAAATTTGAATTCCAGCCCCACATGGGA
GATATGGCCTCTCAACTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTGCAGAGATGCCAACAGCTGC
AGTCTCGCTTATCCACTTTGAAGATTGAGAATGAAGAGGTGAAAAAGACAATGGAGGCCACCCTGCAGAC
CATTCAAGACATTGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAG
TCTGTCAAATCAACTGTCTCTGAAACATTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCGAACCAGC
AAGAGACGGAACAGTTTTATTTCACGAAAATGAAGGAGTACTTGGAGGGTAGGAACCTCATCACTAAGCT
ACAAGCCAAGCATGACCTCCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGACTGTAGTCTTGCCAGG
CGAAGCTCAACTGTGAGGAAACAGGATTCCAGCCAAGCAATTCCTCTGGTGGTAGAAAGCTGCATCCGGT
TTATTAGCAGACATGGCCTACAACATGAGGGAATTTTCCGGGTTTCTGGATCACAAGTAGAAGTGAACGA
CATAAAAAACGCCTTTGAGAGAGGAGAGGATCCCCTGGCTGGGGACCAGAATGACCATGACATGGACTCT
ATAGCTGGTGTCCTCAAGCTTTACTTCCGGGGCCTGGAACACCCGCTCTTCCCTAAAGACATCTTCCATG
ACTTGATTGCCTGTGTCACAATGGACAACCTGCAAGAGAGAGCTGTGCATATCCGGAAAGTCCTTCTGGT
CCTGCCCAAGCCCACTCTGATTATCATGAGATATCTCTTTGCCTTCCTCAATCACTTATCACAGTTCAGT
GAAGAGAACATGATGGACCCCTACAACCTTGCCATCTGCTTCGGGCCCTCACTGATGTCAGTGCCAGAGG
GCCACGACCAGGTGTCCTGTCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAACATGAGAA
CATTTTCCCAAACCCCAGGGAGCTGGAGGGTCCCATCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT
GACAGCACTCATGGAGAGACTACCTCTGCTGAAGACTCCACCCAGGACGTTACAGCGGAGCACCACACAA
GCGATGATGAATGTGAGCCCATAGAAGCCATTGCCAAGTTTGACTACGTAGGCCGGACAGCCCGAGAACT
GTCTTTCAAGAAGGGAGCATCCCTGCTGCTCTACCAGCGAGCTTCTGATGACTGGTGGGAGGGCCGGCAC
AACGGTATAGATGGACTCATCCCCCATCAGTACATCGTAGTCCAAGACACCGAGGACGGTGTCGTGGAGA
GGTCCAGCCCCAAGTCTGAGATTGAGGTCATGTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAACGGG
GGCCAGCTGTCCCAGTGGGGGTCATGTAGCTGATATTTATCTTGCAAACATCAACAAGCAAAGGAAGCGT
CCAGAATCTGGGAGCATCAGAAAAGCATTTCGGAGTGACAGCCATGGGCTGGGCAGTTCTCTGACTGACT
CCTCCTCCCTGGGGGTGGGGGCTAGCTGCCGTCCATCCTCCCAGCCCATCATGAGCCAGAATCTCCCCAA
GGAAGGGCCAGATAAGTGTTCCATCAGCGGCCATGGCAGCCTCAACTCTATCAGCCGCCACTCATCCTTG
AAGAACCGGATGGACAGTCCACAGATCCGGAAGACCGCTACGGCAGGAAGGTCAAAAAGTTTCAATAACC
ATCGGCCCATGGACCCTGAAGTCATTGCACAGGATATTGAAGCAACAATGAACTCTGCCCTGAATGAGCT
TCAAGAGCTAGAGCGGCAGAGCAGTGCTAAGCACACACCTGATGTGGTTCTGGACACCTTGGAACCACTC
AAGACCTCCCCGGTGGTAGCCCCCACATCTGAGCCCTCCAGCCCTCTGCACACCCAGCTCCTCAAGGACC
CTGAGCCTGCCTTCCAGCGCAGCGCTAGTACTGCTGGGGACATTGCCTGCGCCTTCCGGCCTGTAAAGTC
TGTCAAGATGGCTGCTCCAGTCAAACCACCAGCCACACGGCCCAAGCCAACTGTCTTCCCCAAAACAAAC
GCCACTAGCCCTGGTGTCAATTCATCTGCTTCCCCACAGGCCACTGACAAGTCTTGTACTGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217088 representing NM_001081011
Red=Cloning site Green=Tags(s)

MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL
AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK
KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST
ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCC
DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG
DMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME
SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR
RSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS
IAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFS
EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYC
DSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH
NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKR
PESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSL
KNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPL
KTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTN
ATSPGVNSSASPQATDKSCTV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081011
ORF Size 3213 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081011.2, NP_001074480.2
RefSeq Size 8040 bp
RefSeq ORF 3216 bp
Locus ID 14270
UniProt ID Q91Z67
Cytogenetics 1 E4
Summary RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. May play a role for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081011" in other vectors (4)
SKU Description Size Price
MC223445 Srgap2 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10ug) 10 ug
$1,026.00
MR217088 Srgap2 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2) 10 ug
$589.00 $932.00
MR217088L3 Lenti ORF clone of Srgap2 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2) 10 ug
$1,232.00
MR217088L4 Lenti ORF clone of Srgap2 (mGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2) 10 ug
$1,232.00

file-alt Citations

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