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Tssk5 (NM_183099) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217048
Tssk5 (tGFP-tagged) - Mouse testis-specific serine kinase 5 (Tssk5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tssk5
Synonyms 1700091F14Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217048 representing NM_183099
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGCAGCAGCTGGCGCAAGTCGGATCAGAGGGTGTTCATAGAACAGGTTCGAGAATGCATGAACA
ACGGTTACTTGCTCTCCTCCAAGAAGATCGGATCTGGTGCTTTCTCCAAGGTCTACCTGGCCTATGCAAC
ACGTGAGCGAATGAAGCACAACCCTAGGCTATCCTCTGACTTGCGGGGCAAGCGCCACACTATGGTGGCT
ATCAAGATTGTGTCCATGGCTGAAGCCCCGGCGGAATACTCCAGAAAGTTCCTGCCCCGTGAGATTTTAT
CACTCAATGCTACCTACAAGCACATGAACATAGTTCAACTGTATGAGACCTACCAGAACAGCCAGCGCTC
CTACCTGGTACTGGAGTTGGCAGCTAGAGGTGACCTGTTGGAGCATATCAACGCAGTGTCAGACCTCCGA
TGTTGCCCAGGACTGGAGGAGGAAGAAGCCCGAAGGCTGTTCTGGCAACTGGTCAGCGCTGTGGCCCACT
GCCACAATGTTGGCATTGTACACAGGGACTTGAAATGTGAAAATATCCTGTTGGATGACCAGGGCTTCAT
AAAGCTGACAGATTTTGGCTTTGCCAACTGGGTGGGGCTCAAGAACTCCCTGCTGAGCACCTTCTGTGGC
TCGGTGGCCTATACGGCCCCAGAGATCCTCATGAGCAAGAAGTACAACGGTGAGCAGGCTGATCTGTGGA
GCCTAGGGATCATCCTCCATGCTATGGTGTCTGGGAAGCTGCCCTTCAAGGAACACCAGCCCCATCGCAT
GCTGAACCTGATTCGTCGAGGTCCCATCTTCAGGCCAGGGCTGTCCCCAGAGTGCCGGGACCTGATCCGA
GGGCTGCTTCAGCTGCATCCATGTGAACGTCTGGACCTACAGCAGGTAGCTGCTCACTGCTGGATGCTGC
CTGCAGAACACATGCTGTCCTCTGCACTTGGAGCTCCCCGAGAACAGGATCATTCCTGGTCCACAGTAGC
CCCAGACAACACAGAGCCTGACAGAGACACCAGGCATGCCAGAAGCAAAGGCTCCAGCTCCTCCAGTGGC
AGGACATCTCCAAGGAGACCGTCTCTGGCCCAGCTGTGCAACACCTGGAAGCCTGCACCAGAGCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217048 representing NM_183099
Red=Cloning site Green=Tags(s)

MRSSSWRKSDQRVFIEQVRECMNNGYLLSSKKIGSGAFSKVYLAYATRERMKHNPRLSSDLRGKRHTMVA
IKIVSMAEAPAEYSRKFLPREILSLNATYKHMNIVQLYETYQNSQRSYLVLELAARGDLLEHINAVSDLR
CCPGLEEEEARRLFWQLVSAVAHCHNVGIVHRDLKCENILLDDQGFIKLTDFGFANWVGLKNSLLSTFCG
SVAYTAPEILMSKKYNGEQADLWSLGIILHAMVSGKLPFKEHQPHRMLNLIRRGPIFRPGLSPECRDLIR
GLLQLHPCERLDLQQVAAHCWMLPAEHMLSSALGAPREQDHSWSTVAPDNTEPDRDTRHARSKGSSSSSG
RTSPRRPSLAQLCNTWKPAPEQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183099
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183099.2, NP_898922.2
RefSeq Size 1514 bp
RefSeq ORF 1119 bp
Locus ID 73542
UniProt ID Q8C1R0
Cytogenetics 15 D3
Summary May be involved in a signaling pathway during male germ cell development or mature sperm function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183099" in other vectors (4)
SKU Description Size Price
MC211435 Tssk5 (untagged) - Mouse testis-specific serine kinase 5 (Tssk5), (10ug) 10 ug
$503.00
MR217048 Tssk5 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 5 (Tssk5) 10 ug
$289.00 $457.00
MR217048L3 Lenti ORF clone of Tssk5 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 5 (Tssk5) 10 ug
$757.00
MR217048L4 Lenti ORF clone of Tssk5 (mGFP-tagged) - Mouse testis-specific serine kinase 5 (Tssk5) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.