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Pdpr (NM_198308) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217047
Pdpr (tGFP-tagged) - Mouse RIKEN cDNA 4930402E16 gene (4930402E16Rik), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,005.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdpr
Synonyms 4930402E16Rik; AU018056; mKIAA1990
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217047 representing NM_198308
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTTACCGGTTGCTCTCTATTGTGCAAAGACAAAGGACTAGCCCAGGATGGCAGACCTGGTCATCAG
CGAGAAGCAGCACGTCCACTGCGGAGGCACATTCTATTGCTCTGCCTGCCCAGGCCCAGGTGGTCATCTG
TGGAGGTGGAATCATGGGTACATCCGTGGCCTATCACCTCTCCAAAATGGGATGGCAAGATATTGTCCTT
TTGGAGCAGGGCAGGCTGGCTGCTGGCTCAACAAGGTTCTGTGCTGGCATCCTAAGCACTGCTAGGCATT
CAAGCGTTGAGCAGAAGATGGCTAACTATTCAAACAAACTCTACCATCAGTTAGAACAAGAAACAGGGAT
CCAAACAGGTTACTTAAGGACAGGCTCAATCTCTCTGGCCCAGACTCAGGACCGGTTGATCTCTCTGAAG
CGTATCAACTCAAGGCTGAATGTTGTAGGTATCCCTTCTGAGATCATCTCCCCGAAGAAAGTGGCTGAAC
TTCACCCTCTCCTCAATGTGCATGATCTGGTCGGGGCCATGTACGTTCCTGAGGATGCAGTAGTGTCCTC
TGCTGATGTGGCTCTGGCCCTGGCAAGTGCTGCCTCCCAGAATGGTGTTCAGATTTATGACCGGACAAGT
GTTCTTCACGTACTGATCAAAAAAGGTCAAGTTACTGGTGTGGAGACAGACAAAGGACAGATCGAATGCC
AGTATTTTGTCAACTGTGCCGGGCAGTGGGCATATGAGCTGGGTCTGTCCAACGAGGAGCCGCTTAGTAT
CCCGCTACACGCCTGTGAACACTTCTACCTTCTGACTCGTCCCTGGGACACCCCTCTGCAGAGCAACACA
CCAACCATTGTGGATGCTGATGGAAGAATTTACATTAGGAACTGGCAGGGTGGCATCTTGTCTGGAGGCT
TTGAGAAGAACCCCAAACCTATTTTCACTGAAGGCAAGAACCAGTTGGAAATTCAGAACCTTCGTGAAGA
CTGGGATCACTTTGAGCCCCTGCTGAGTTCCCTCCTGCGTAGGATGCCAGCATTGGAGACTCTGGAGATT
TTGAAGTTGGTGAATTGCCCTGAGACCTTCACACCAGACATGAAGTGCATCATGGGCGAGTCTCCTGTAG
TACAGGGCTACTTTGTCCTGGCAGGGATGAACTCTGCTGGCCTGTCGTTGGGTGGAGGAGCTGGAAAGTT
CCTAGCAGAATGGATGGTATATGGTTATCCCTCTGAAAATGTCTGGGAGTTGGATTTGCAGCGCTTCGGA
GCCCTCCAGAGCAGCCGCACCTTTCTGCGCCACCGGGTCATGGAAGTTATGCCTCTGATATATGATCTGA
AGGTTCCCCGTTGGGATTTCCAGACTGGAAGGCAGTTACGTACATCTCCTCTTTATGACCGGCTGGATGC
TCAAGGAGCCAGATGGATGGAGAAACATGGATTTGAGAGGCCAAAGTACTTTGTTCCACCCAACAAGGAC
CTTCTTGCATTAGAACAGAGCAAGACTTTCTACAAGCCAGATTGGTTTGATATTGTGGAGTCAGAAGTCA
AATGCTGCAAGGAAGCTGTGTGTGTCATTGACATGTCCTCTTTCACAAAATTTGAAATAACTTCCACTGG
GGACGAGGCGTTAGAAAGTCTGCAGTACCTTTTCTGCAATGACCTTGATGTACCTGTAGGCCACATCGTT
CATACTGGCATGCTCAATGAATATGGAGGATACGAAAACGACTGCAGCATCGCACGCCTGACCAAACGCA
GTTTCTTTATGATTTCTCCAACCGATCAGCAGGTCCACTGTTGGGCCTGGCTTAACAAATACTTGCCGAA
AGACAGCAACTTGCTGCTGGAGGACGTCACCTGGAAATACACAGCCCTCAATCTGATTGGCCCTCGAGCC
GTGGATGTGCTATCTGAGCTGTCCTATGCCCCTATGACTCCAGATCACTTTCCAACGCTCTTCTGCAAGG
AGATGAGTGTGGGCTATGCAAATGGGATCCGGGTGATGAGTATGACGCACACGGGAGAGCCAGGCTTCAT
GCTGTATATCCCCATAGAGTACGCTCTGCATGTCTACAATGAGGTGATGAGTGTTGGACAGAAATATGGA
ATCCGGAATGCTGGGTATTATGCCCTCAGAAGTCTCCGAATTGAGAAGTTTTTTGCTTTCTGGGGTCAGG
ACTTAAACACCCTTACCACGCCTTTGGAATGTGGAGGAGAATCTCGAGTGAAGTTAGAGAAGGGCATAGA
CTTCATTGGTCGGGATGCCCTGCTGCAGCAGAAGCAGACCGGGGTATATAAGCGCCTTGCTATGTTCATT
CTGGACGACCATGACACAGACCTTGATTTGTGGCCTTGGTGGGGAGAACCCATTTACCGGAACGGAAAGT
ATGCTGGCAAGACCACCAGTAGTGCCTATAGCTACACACTGGAGCGCCATGTATGCCTGGGTTATGTACA
CAATTTTTCTGAGGACAGTGGGGAAGAGCAGGTGGTGACAACAGATTTCATCAACCGAGGAGAATATGAA
ATTGACATTGCAGGACATCGGTTTCAGGCCAAGGCCAAGCTCTACCCTGTCACCTCTTTTTTCACTCACA
AGCGTAGAAAGGACGATGTGGAGCTGAGCGACTTTCATGGGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217047 representing NM_198308
Red=Cloning site Green=Tags(s)

MLYRLLSIVQRQRTSPGWQTWSSARSSTSTAEAHSIALPAQAQVVICGGGIMGTSVAYHLSKMGWQDIVL
LEQGRLAAGSTRFCAGILSTARHSSVEQKMANYSNKLYHQLEQETGIQTGYLRTGSISLAQTQDRLISLK
RINSRLNVVGIPSEIISPKKVAELHPLLNVHDLVGAMYVPEDAVVSSADVALALASAASQNGVQIYDRTS
VLHVLIKKGQVTGVETDKGQIECQYFVNCAGQWAYELGLSNEEPLSIPLHACEHFYLLTRPWDTPLQSNT
PTIVDADGRIYIRNWQGGILSGGFEKNPKPIFTEGKNQLEIQNLREDWDHFEPLLSSLLRRMPALETLEI
LKLVNCPETFTPDMKCIMGESPVVQGYFVLAGMNSAGLSLGGGAGKFLAEWMVYGYPSENVWELDLQRFG
ALQSSRTFLRHRVMEVMPLIYDLKVPRWDFQTGRQLRTSPLYDRLDAQGARWMEKHGFERPKYFVPPNKD
LLALEQSKTFYKPDWFDIVESEVKCCKEAVCVIDMSSFTKFEITSTGDEALESLQYLFCNDLDVPVGHIV
HTGMLNEYGGYENDCSIARLTKRSFFMISPTDQQVHCWAWLNKYLPKDSNLLLEDVTWKYTALNLIGPRA
VDVLSELSYAPMTPDHFPTLFCKEMSVGYANGIRVMSMTHTGEPGFMLYIPIEYALHVYNEVMSVGQKYG
IRNAGYYALRSLRIEKFFAFWGQDLNTLTTPLECGGESRVKLEKGIDFIGRDALLQQKQTGVYKRLAMFI
LDDHDTDLDLWPWWGEPIYRNGKYAGKTTSSAYSYTLERHVCLGYVHNFSEDSGEEQVVTTDFINRGEYE
IDIAGHRFQAKAKLYPVTSFFTHKRRKDDVELSDFHGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198308
ORF Size 2634 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198308.1, NP_938050.1
RefSeq Size 2904 bp
RefSeq ORF 2637 bp
Locus ID 319518
UniProt ID Q7TSQ8
Cytogenetics 8 E1
Summary Decreases the sensitivity of PDP1 to magnesium ions, and this inhibition is reversed by the polyamine spermine.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198308" in other vectors (4)
SKU Description Size Price
MC222353 Pdpr (untagged) - Mouse pyruvate dehydrogenase phosphatase regulatory subunit (Pdpr), (10ug) 10 ug
$885.00
MR217047 Pdpr (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase phosphatase regulatory subunit (Pdpr) 10 ug
$589.00 $805.00
MR217047L3 Lenti ORF clone of Pdpr (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase phosphatase regulatory subunit (Pdpr) 10 ug
$1,105.00
MR217047L4 Lenti ORF clone of Pdpr (mGFP-tagged) - Mouse pyruvate dehydrogenase phosphatase regulatory subunit (Pdpr) 10 ug
$1,105.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.