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Wdr43 (NM_175639) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217034
Wdr43 (tGFP-tagged) - Mouse WD repeat domain 43 (Wdr43), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $820.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr43
Synonyms 2610318G08Rik; AU020887; AV024208; mKIAA0007; Wrd43
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217034 representing NM_175639
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGGAGGCGGCGGTAGCTACGACCCCTTGGCCCCGGCGGGGGTCCCCTGCGCCTTCTCGCCGG
ACAGTCAAGCCTACTTCGCTTTAGCCTCTAGTGACGGTCAGCTGCGAGTATGGGAGACCGCCAACAACCG
GCTGCACCAGGAGTACGTGCCTTCCGCGCACCTCAGCGGTACCTGCACCTGCCTGGCCTGGGCGCCAGCA
CGGCTGCAGGCCAAGGAAAGTCATCAAAGGAAAAAAAGGAAATCGGAAGTTACAGGAACAAAGGACCAGG
CTGACCTGTTGGCTCTTGGCACGGCAGTTGGTAGCATTTTATTGTATAGTACAGTAAGAGGAGAGTTGCA
CAGTAAATTAACAAGTGGTGGGCATGAAAACAGAGTGAATTGCATACAGTGGCATCAAGACAATGACTGC
TTATATAGTTGTTCAGATGATAAGTATATTGTGGAGTGGAGTACACAGACATGCAAAGTAAAGTGCAAAT
GGAAAGGAGACAATAGCAGCGTCAGTTCCCTGTGTATCAGCCCAGATGGAAAGATGTTACTTTCAGCTGG
TCGAACAATCAAACTATGGGTTTTGGAGACCAAAGAAGTCTACAGACACTTCACAGGACATGCGACACCT
GTTTCGTCGCTTAGGTTCACGACCATCAGACCTAATGAGAGCCAGCCTTCTGATGGGATTACAGGCCTTT
ATTTCTTGTCTGGAGCCGTACACGACCGATTACTTAATGTCTGGCAGGTCCGGTCAGAGAACAAGGAGAA
GAGTGCAGTGATGTCTTTTACAGTCACAGACGAGCCTGTCTATGTTGACTTGACTTTGTCAGAAAACAAA
GAAGAGCCTGTCAAGTTAGCTGTTGTTTGCAGAGATGGTCAAGTTCATCTTTTTGAACACATATTAAATG
GACATTGCAAAAAGCCCTTGACTTCAAATTGCACAATCCAGATCGCAACACCTGGAAAGGGCAAGAAAGT
AACACCAAAACCCATCCCTATTCTAGCAGCTAGCTTTTGCTTAGACAAAATGTCCTTATTGCTGGTGTAT
GGAAATTGGTTTCAGCCTACCATTGAGCGAGTGGCTTTGAACTCCAAAGATACTCATATATGTTTGGAAA
GAGATATTTCAAATTGCTGGGCTCCAACAGTAGAAACTGCTATAACCAAGGTAAAAACACCAGTAATGAA
TTCTGAAGCAAAAGTTTTGGTGCCCGGGATTCCTGGACATCATGCGCCAATCAAGCTGCCGCCCGCCCAG
CCCAAGGAGGCCGAGAACAAGAGGAAATTGGGCAGTACTGAGGCTACCATCGAGGAGCGCCTTGGTGCCA
TGGACCTAGATAGAAAAGGAAGGAAGGACGACCTTCAGACAAACAGCTTTGCAGTCCTCCTTACACAGGG
CTTAGAAAGCAATGATTTTGAAATATTAAACAAAGTGCTTCAAACTAAGAATGTAAACCTAATAAAGAGG
ACTGTGCTGAGGATACCGTTGCGTGTTGTTATTCCACTGTTGCAAGAGCTTACGAAGAGGTTACAGGGAC
ATCCCAATAGTGCTGCTTTAATGATTCAGTGGCTGAAGTGTGTGCTGACGATCCATGCATCATACCTATC
TACGTTGCCTGACCTAGTAGAGCAGCTGGGAACACTCTACCAGCTGATGGAAAGCAGAGTCAAAACTTTC
CAGAAACTCTCAAACCTGCATGGAAAACTCATTCTTCTCGTCACGCAAGTTACAGCATCAGAAAAATCGA
AAAAAATGACCTCTCCCGGACAGAAGGCAAAATTGGTGTATGAAGAAGAGTCTTCTGAGGAGGAGTCTGA
CGATGAGGTTCCAGAGAAGGATTCTGATGATAATTGGGACGAAGATGAAGACAAAGACAGTGAAAAAGAT
GAGGGCGTTGATGAAGACAATGAGGAGGAAGATGAGGACATGGAAGACAAAGAAGAGAATGAGGAGGACA
GAGAAGTGTCAAGTGAAAAAGAATTAAATGGAGATTCGGATTTAGATCCTGAAAACGAGAGTGAAGAAGA
A

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217034 representing NM_175639
Red=Cloning site Green=Tags(s)

MAAGGGGSYDPLAPAGVPCAFSPDSQAYFALASSDGQLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPA
RLQAKESHQRKKRKSEVTGTKDQADLLALGTAVGSILLYSTVRGELHSKLTSGGHENRVNCIQWHQDNDC
LYSCSDDKYIVEWSTQTCKVKCKWKGDNSSVSSLCISPDGKMLLSAGRTIKLWVLETKEVYRHFTGHATP
VSSLRFTTIRPNESQPSDGITGLYFLSGAVHDRLLNVWQVRSENKEKSAVMSFTVTDEPVYVDLTLSENK
EEPVKLAVVCRDGQVHLFEHILNGHCKKPLTSNCTIQIATPGKGKKVTPKPIPILAASFCLDKMSLLLVY
GNWFQPTIERVALNSKDTHICLERDISNCWAPTVETAITKVKTPVMNSEAKVLVPGIPGHHAPIKLPPAQ
PKEAENKRKLGSTEATIEERLGAMDLDRKGRKDDLQTNSFAVLLTQGLESNDFEILNKVLQTKNVNLIKR
TVLRIPLRVVIPLLQELTKRLQGHPNSAALMIQWLKCVLTIHASYLSTLPDLVEQLGTLYQLMESRVKTF
QKLSNLHGKLILLVTQVTASEKSKKMTSPGQKAKLVYEEESSEEESDDEVPEKDSDDNWDEDEDKDSEKD
EGVDEDNEEEDEDMEDKEENEEDREVSSEKELNGDSDLDPENESEEE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175639
ORF Size 2031 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175639.1, NP_783570.1
RefSeq Size 3450 bp
RefSeq ORF 2034 bp
Locus ID 72515
UniProt ID Q6ZQL4
Cytogenetics 17 E1.3
Summary Ribosome biogenesis factor. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175639" in other vectors (4)
SKU Description Size Price
MC220351 Wdr43 (untagged) - Mouse WD repeat domain 43 (Wdr43), (10ug) 10 ug
$683.00
MR217034 Wdr43 (Myc-DDK-tagged) - Mouse WD repeat domain 43 (Wdr43) 10 ug
$589.00 $620.00
MR217034L3 Lenti ORF clone of Wdr43 (Myc-DDK-tagged) - Mouse WD repeat domain 43 (Wdr43) 10 ug
$920.00
MR217034L4 Lenti ORF clone of Wdr43 (mGFP-tagged) - Mouse WD repeat domain 43 (Wdr43) 10 ug
$920.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.