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Pcid2 (NM_178708) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217027
Pcid2 (tGFP-tagged) - Mouse PCI domain containing 2 (Pcid2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcid2
Synonyms A730042J05Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217027 representing NM_178708
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCACATCACCATTAACCAGTACCTACAGCAGGTATATGAAGCCATCGACACCAGAGATGGAGCAT
CCTGTGCAGAGCTGGTATCCTTTAAGCACCCTCATGTTGCGAACCCACGGCTCCAGATGGCCTCTCCAGA
AGAGAAATGCCAGCAAGTTCTGGAGCCCCCATATGATGAAATGTTTGCAGCTCATTTAAGGTGTACCTAT
GCTGTGGGGAATCACGACTTCATAGAAGCGTACAAGTGCCAGACGGTGATAGTCCAATCATTCCTCCGGG
CATTCCAGGCTCACAAAGAGGAAAACTGGGCTCTGCCTGTCATGTATGCAGTAGCACTTGACCTTCGGAT
ATTTGCCAATAATGCAGACCAGCAGTTGGTAAAGAAAGGGAAGAGCAAAGTTGGAGACATGCTGGAAAAA
GCTGCCGAGCTGCTGATGAGCTGCTTCCGAGTCTGTGCCAGTGACACCCGTGCCGGCATAGAAGACTCTA
AGAAGTGGGGGATGCTGTTCCTGGTGAACCAGCTATTTAAAATCTACTTTAAGATCAACAAACTCCATTT
GTGTAAACCTCTTATCAGAGCCATCGACAGCTCGAATCTGAAAGATGACTATAGCACAGCGCAGAGGATC
ACGTACAAGTACTACGTGGGGCGCAAGGCCATGTTTGACAGTGACTTCAAGCAAGCGGAGGAGTACCTGT
CGTTTGCCTTTGAACACTGCCATCGCTCCAGCCAGAAGAACAAGAGGATGATTCTGATCTACCTGCTGCC
AGTGAAGATGCTGCTGGGTCACATGCCGACCATCGAGCTCCTGAGGAAATACCACCTGATGCAGTTTTCA
GAAGTGACCAAAGCCGTCAGTGAGGGCAACCTGCTGCTGCTCAATGAGGCGCTGGCAAAGCACGAGACGT
TCTTCATCCGCTGTGGCATCTTCCTCATCCTGGAGAAGCTGAAGATCATCACGTACAGGAACCTCTTCAA
GAAAGTGTATTTGTTACTCAAGACACATCAGCTGTCCCTAGACGCCTTTCTGGTCGCTTTGAAGTTCATG
CACGTGGAAGACGTGGACATTGACGAGGTCCAGTGCATTCTGGCCAACTTGATATACATGGGTCACATTA
AAGGCTACATCTCCCATCAGCATCAGAAACTGGTCGTCAGCAAGCAGAACCCTTTCCCTCCGCTGTCCAC
AGTGTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217027 representing NM_178708
Red=Cloning site Green=Tags(s)

MAHITINQYLQQVYEAIDTRDGASCAELVSFKHPHVANPRLQMASPEEKCQQVLEPPYDEMFAAHLRCTY
AVGNHDFIEAYKCQTVIVQSFLRAFQAHKEENWALPVMYAVALDLRIFANNADQQLVKKGKSKVGDMLEK
AAELLMSCFRVCASDTRAGIEDSKKWGMLFLVNQLFKIYFKINKLHLCKPLIRAIDSSNLKDDYSTAQRI
TYKYYVGRKAMFDSDFKQAEEYLSFAFEHCHRSSQKNKRMILIYLLPVKMLLGHMPTIELLRKYHLMQFS
EVTKAVSEGNLLLLNEALAKHETFFIRCGIFLILEKLKIITYRNLFKKVYLLLKTHQLSLDAFLVALKFM
HVEDVDIDEVQCILANLIYMGHIKGYISHQHQKLVVSKQNPFPPLSTVC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178708
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178708.3, NP_848823.2
RefSeq Size 2408 bp
RefSeq ORF 1200 bp
Locus ID 234069
UniProt ID Q8BFV2
Cytogenetics 8 A1.1
Summary Required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation (PubMed:20870947). As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (By similarity). Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and transcription-associated genomic instability. R-loop accumulation does not increase in PCID2-depleted cells (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178708" in other vectors (4)
SKU Description Size Price
MC212980 Pcid2 (untagged) - Mouse PCI domain containing 2 (Pcid2), (10ug) 10 ug
$503.00
MR217027 Pcid2 (Myc-DDK-tagged) - Mouse PCI domain containing 2 (Pcid2) 10 ug
$289.00 $457.00
MR217027L3 Lenti ORF clone of Pcid2 (Myc-DDK-tagged) - Mouse PCI domain containing 2 (Pcid2) 10 ug
$757.00
MR217027L4 Lenti ORF clone of Pcid2 (mGFP-tagged) - Mouse PCI domain containing 2 (Pcid2) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.