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Ccbe1 (NM_178793) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217003
Ccbe1 (tGFP-tagged) - Mouse collagen and calcium binding EGF domains 1 (Ccbe1), (10ug)
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ORF Plasmid:
tGFP DDK
  $886.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccbe1
Synonyms 4933426F18Rik; 9430093N24Rik; mKIAA1983
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217003 representing NM_178793
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCGCCGCCTCTTCCGAGCCGGGGAGGAGCAGCCAAGAGGCAGCTGGGCAAGAGCCTGGGGCCGC
TGCTGCTACTCCTGGCGCTGGGACACACGTGGACCTACCGAGAGGAACCCGAGGACCGCGACAGAGAGGT
GTGCTCTGAAAACAAGATCACCACGACCAAATACCCATGTCTGAAGTCTTCGGGCGAGCTCACCACGTGC
TTCAGGAAAAAATGCTGCAAAGGATATAAATTTGTTCTTGGACAGTGTATTCCAGAAGATTATGACATTT
GTGCCCAAGCTCCCTGTGAACAGCAGTGCACGGATAACTTCGGCCGTGTGCTGTGCACGTGCTACCCGGG
ATACCGATATGACCGCGAGAGACACCAAAAGCGGGAGAGACCGTACTGTCTGGATATCGATGAATGTGCC
ACCAGCAATACCACTCTCTGTGCACATATCTGCATCAACACCATGGGCAGCTACCACTGTGAGTGTCGGG
AGGGCTACATCCTTGAGGACGATGGGAGGACCTGCACAAGGGGAGACAAATACCCCAATGACACTGGGCA
TGAAGAGAAGTCCGAGAATGAGGTGAAAGCCGGGACCTGCTGCGCCACATGCAAAGAGTTCTCCCAGATG
AAGCAGACCGTGCTGCAGCTGAAACAGAAGATGGCGCTGCTCCCCAACAATGCCGCTGAACTGGGCAAGT
ATGTCAATGGTGACAAGGTACTGGCCTCAAACGCCTACCTTCCAGGACCTCCTGGCCTGCCTGGGGGACA
GGGCCCACCAGGCTCACCAGGACCCAAGGGGAGCCCTGGATTCCCCGGTATGCCAGGCCTTCCTGGACAG
CCAGGCCCACGGGGCTCAATGGGACCTATGGGACCTTCCCCTGACCTGTCTCATATTAAGCAAGGCCGGA
GGGGCCCCGTGGGCCCACCAGGAGCACCAGGAAGACACGGCTCCAAGGGGGAGAGAGGAGCACCCGGACC
CCCGGGGTCTCCGGGACCCCCAGGTTCTTTCGACTTCCTGCTGCTTGTGCTGGCGGACATTCGAAATGAC
ATCGCAGAGCTGCAGGAGAAGGTGTTTGGACACCGGACTCACTCTTCCGCCGAGGACTTCCCCCTACCTC
AGGAATTCTCCAGCTACCCAGAGACGCTGGACTTTGGCTCTGGAGATGACTACTCCAGAAGAACTGAAGC
AAGGGACCCCGAGGCCCCAAGAAATTTTTATCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217003 representing NM_178793
Red=Cloning site Green=Tags(s)

MVPPPLPSRGGAAKRQLGKSLGPLLLLLALGHTWTYREEPEDRDREVCSENKITTTKYPCLKSSGELTTC
FRKKCCKGYKFVLGQCIPEDYDICAQAPCEQQCTDNFGRVLCTCYPGYRYDRERHQKRERPYCLDIDECA
TSNTTLCAHICINTMGSYHCECREGYILEDDGRTCTRGDKYPNDTGHEEKSENEVKAGTCCATCKEFSQM
KQTVLQLKQKMALLPNNAAELGKYVNGDKVLASNAYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGLPGQ
PGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRHGSKGERGAPGPPGSPGPPGSFDFLLLVLADIRND
IAELQEKVFGHRTHSSAEDFPLPQEFSSYPETLDFGSGDDYSRRTEARDPEAPRNFYP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178793
ORF Size 1224 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178793.4, NP_848908.1
RefSeq Size 5742 bp
RefSeq ORF 1227 bp
Locus ID 320924
UniProt ID Q3MI99
Cytogenetics 18 E1
Summary Required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178793" in other vectors (2)
SKU Description Size Price
MC214489 Ccbe1 (untagged) - Mouse collagen and calcium binding EGF domains 1 (Ccbe1), (10ug) 10 ug
$732.00
MR217003 Ccbe1 (Myc-DDK-tagged) - Mouse collagen and calcium binding EGF domains 1 (Ccbe1) 10 ug
$686.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.