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Mcpt1 (NM_008570) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216930
Mcpt1 (tGFP-tagged) - Mouse mast cell protease 1 (Mcpt1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcpt1
Synonyms AV080368; Mcp-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216930 representing NM_008570
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCCCTACTATTCCTGATGGCACTTCTCTTGCCTTCTGGAGCTGGAGCTGAGGAGATTATTGGTG
GTGTTGAGGCTAGACCACACTCCCGTCCTTACATGGCCCATCTGAAGATCATCACGGACAGAGGTTCTGA
GGACAGATGTGGTGGGTTTCTCATAGCCCCCCAATTTGTGTTGACTGCTGCACACTGTAAAGGAAGAGAA
ATCACTGTCACCCTTGGAGCTCATGATGTGAGCAAGAGTGAATCCACACAACAGAGGATAAAAGTCGAAA
AACAAATCATTCACAAAAATTACAACGTCTCTTTCAATCTCTATGACATCATGTTACTGAAGCTTGAAGA
GAAAGCTGAGTTGACTCCTACTGTGGATGTAATTCCCTTGCCTGGTCCCTCTGACTTTATCGACCCTGGG
AAGATGTGCTGGACAGCTGGCTGGGGGAAAACTGGAGAGAAAGAACCTACCTCAGAAACCCTGAGAGAGG
TTGAACTGAGAATCATGGATAAAGAGGCCTGTAAAATGTATAAGCATTATGACTATAACTTCCAGGTCTG
TGTGGGAAGTTCCACAAAGTTAAAAACAGCATACATGGGAGACTCTGGGGGACCTCTTCTGTGTGCTGGT
GTGGCCCATGGTATTGTATCTTATGGGGATTCACATGGAAAGCCCCCTGCAGTCTTCACCAGAATCTCTG
CATATGTGCCCTGGATTAAAACAGTCATAAATGGCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216930 representing NM_008570
Red=Cloning site Green=Tags(s)

MQALLFLMALLLPSGAGAEEIIGGVEARPHSRPYMAHLKIITDRGSEDRCGGFLIAPQFVLTAAHCKGRE
ITVTLGAHDVSKSESTQQRIKVEKQIIHKNYNVSFNLYDIMLLKLEEKAELTPTVDVIPLPGPSDFIDPG
KMCWTAGWGKTGEKEPTSETLREVELRIMDKEACKMYKHYDYNFQVCVGSSTKLKTAYMGDSGGPLLCAG
VAHGIVSYGDSHGKPPAVFTRISAYVPWIKTVINGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008570
ORF Size 738 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008570.1, NP_032596.1
RefSeq Size 1028 bp
RefSeq ORF 741 bp
Locus ID 17224
UniProt ID P11034
Cytogenetics 14 28.19 cM
Summary Has a chymotrypsin-like activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008570" in other vectors (4)
SKU Description Size Price
MC208949 Mcpt1 (untagged) - Mouse mast cell protease 1 (Mcpt1), (10ug) 10 ug
$330.00
MR216930 Mcpt1 (Myc-DDK-tagged) - Mouse mast cell protease 1 (Mcpt1) 10 ug
$289.00 $300.00
MR216930L3 Lenti ORF clone of Mcpt1 (Myc-DDK-tagged) - Mouse mast cell protease 1 (Mcpt1) 10 ug
$600.00
MR216930L4 Lenti ORF clone of Mcpt1 (mGFP-tagged) - Mouse mast cell protease 1 (Mcpt1) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.