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Timm8a2 (NM_001037744) Mouse Tagged ORF Clone

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SKU
MG216924
Timm8a2 (tGFP-tagged) - Mouse translocase of inner mitochondrial membrane 8 homolog a2 (yeast) (Timm8a2) nuclear gene encoding mitochondrial protein, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Timm8a2
Synonyms MGC117751
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216924 representing NM_001037744
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCGCCTGGTCTTCCCGGGGGACCAGCCTGGGATCTTCAGACCCGCAGCTGCAGCGGTTCATGG
AGGCAGAGGTGCAGAAGCAGAGGGTGCAGTTGCTCATCCACCACATGACAGAGCTCTGCTGGGAGAAGTG
CATGGACAAGCCCGGGCCCAGGCTGGATGGCCGGGCGGAGCTCTGCTTGGTGAACTGCGTCGAGCGCTTC
ATTGATACGAGCCAGTTCATCTTGAATCGACTGGAGCAGACCCAGAAGGCGAGACCACTCTTCTCAGAAA
GACTTTCCGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216924 representing NM_001037744
Red=Cloning site Green=Tags(s)

MESAWSSRGTSLGSSDPQLQRFMEAEVQKQRVQLLIHHMTELCWEKCMDKPGPRLDGRAELCLVNCVERF
IDTSQFILNRLEQTQKARPLFSERLSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001037744
ORF Size 291 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001037744.1, NP_001032833.1
RefSeq Size 946 bp
RefSeq ORF 294 bp
Locus ID 223262
UniProt ID Q4FZG7
Cytogenetics 14 E5
Summary Putative mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001037744" in other vectors (4)
SKU Description Size Price
MC212707 Timm8a2 (untagged) - Mouse translocase of inner mitochondrial membrane 8 homolog a2 (yeast) (Timm8a2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$165.00
MR216924 Timm8a2 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 8 homolog a2 (yeast) (Timm8a2), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR216924L3 Lenti ORF clone of Timm8a2 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 8 homolog a2 (yeast) (Timm8a2), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR216924L4 Lenti ORF clone of Timm8a2 (mGFP-tagged) - Mouse translocase of inner mitochondrial membrane 8 homolog a2 (yeast) (Timm8a2), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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